BMRB: 11475

Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT

Authors

Tochio, N., Umehara, T., Kigawa, T., Yokoyama, S.

Assembly

protein

Entity

1. protein (polymer, Thiol state: all other bound), 36 monomers, 3805.262 Da Detail

GSSGSSGKPY KCPQCSYASA IKANLNVHLR KHTGEK

2. ZINC ION (non-polymer), 65.409 Da

Total weight

3870.671 Da

Max. entity weight

3805.262 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS12:SG	2:ZN1:ZN
2	na	sing	1:CYS15:SG	2:ZN1:ZN
3	na	sing	1:HIS28:NE2	2:ZN1:ZN
4	na	sing	1:HIS32:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 86.1 %, Completeness: 84.4 %, Completeness (bb): 75.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	84.4 % (336 of 398)	85.2 % (179 of 210)	85.4 % (129 of 151)	75.7 % (28 of 37)
Backbone	75.9 % (161 of 212)	73.0 % (54 of 74)	78.8 % (82 of 104)	73.5 % (25 of 34)
Sidechain	87.2 % (190 of 218)	86.0 % (117 of 136)	88.6 % (70 of 79)	100.0 % (3 of 3)
Aromatic	100.0 % (24 of 24)	100.0 % (12 of 12)	100.0 % (12 of 12)
Methyl	95.8 % (23 of 24)	91.7 % (11 of 12)	100.0 % (12 of 12)

1. protein

GSSGSSGKPY KCPQCSYASA IKANLNVHLR KHTGEK

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 296 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	protein	[U-13C; U-15N]	protein	1.09 mM
2	TRIS	[U-2H]		20 mM
3	sodium chloride	natural abundance		100 mM
4	DTT	[U-2H]		1 mM
5	sodium azide	natural abundance		0.02 %
6	zinc chloride	natural abundance		50 uM
7	H2O		solvent	90 %
8	D2O		solvent	10 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2RUU, Strand ID: A Detail

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Release date

2015-01-19

Citation

Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT
Tochio, N., Umehara, T., Kigawa, T., Yokoyama, S.
J. Struct. Funct. Genomics (2015), 16, 55-65, PubMed 25801860 , DOI 10.1007/s10969-015-9196-3 , Abstract

Related entities 1. protein, : 1 : 3 : 1607 entities Detail

Interaction partners 1. protein, : 2 interactors Detail

Experiments performed 2 experiments Detail