BMRB: 11476

Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT

Authors

Tochio, N., Umehara, T., Kigawa, T., Yokoyama, S.

Assembly

protein

Entity

1. protein (polymer, Thiol state: all other bound), 37 monomers, 4184.676 Da Detail

GSSGSSGIKQ HCRFCKKKYS DVKNLIKHIR DAHDPQD

2. ZINC ION (non-polymer), 65.409 Da

Total weight

4250.085 Da

Max. entity weight

4184.676 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS12:SG	2:ZN1:ZN
2	na	sing	1:CYS15:SG	2:ZN1:ZN
3	na	sing	1:HIS28:NE2	2:ZN1:ZN
4	na	sing	1:HIS33:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.2 %, Completeness: 91.0 %, Completeness (bb): 85.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.0 % (394 of 433)	92.2 % (213 of 231)	90.8 % (148 of 163)	84.6 % (33 of 39)
Backbone	85.0 % (187 of 220)	84.2 % (64 of 76)	86.1 % (93 of 108)	83.3 % (30 of 36)
Sidechain	96.4 % (238 of 247)	96.1 % (149 of 155)	96.6 % (86 of 89)	100.0 % (3 of 3)
Aromatic	100.0 % (30 of 30)	100.0 % (15 of 15)	100.0 % (15 of 15)
Methyl	100.0 % (22 of 22)	100.0 % (11 of 11)	100.0 % (11 of 11)

1. protein

GSSGSSGIKQ HCRFCKKKYS DVKNLIKHIR DAHDPQD

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 296 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	protein	[U-13C; U-15N]	protein	1.09 mM
2	TRIS	[U-2H]		20 mM
3	sodium chloride	natural abundance		100 mM
4	DTT	[U-2H]		1 mM
5	sodium azide	natural abundance		0.02 %
6	zinc chloride	natural abundance		50 uM
7	H2O		solvent	90 %
8	D2O		solvent	10 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2RUW, Strand ID: A Detail

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Release date

2015-01-19

Citation

Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT
Tochio, N., Umehara, T., Kigawa, T., Yokoyama, S.
J. Struct. Funct. Genomics (2015), 16, 55-65, PubMed 25801860 , DOI 10.1007/s10969-015-9196-3 , Abstract

Related entities 1. protein, : 1 : 1 : 6 : 32 entities Detail

Experiments performed 2 experiments Detail