BMRB: 11478

Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT

Authors

Tochio, N., Umehara, T., Kigawa, T., Yokoyama, S.

Assembly

protein

Entity

1. protein (polymer, Thiol state: free and other bound), 37 monomers, 4137.641 Da Detail

GSSGSSGHLY YCSQCHYSSI TKNCLKRHVI QKHSNIL

2. ZINC ION (non-polymer), 65.409 Da

Total weight

4203.0503 Da

Max. entity weight

4137.641 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS12:SG	2:ZN1:ZN
2	na	sing	1:CYS15:SG	2:ZN1:ZN
3	na	sing	1:HIS28:NE2	2:ZN1:ZN
4	na	sing	1:HIS33:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 83.8 %, Completeness: 82.7 %, Completeness (bb): 76.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	82.7 % (349 of 422)	83.6 % (183 of 219)	85.8 % (139 of 162)	65.9 % (27 of 41)
Backbone	76.1 % (169 of 222)	72.7 % (56 of 77)	81.5 % (88 of 108)	67.6 % (25 of 37)
Sidechain	89.7 % (210 of 234)	89.4 % (127 of 142)	92.0 % (81 of 88)	50.0 % (2 of 4)
Aromatic	90.0 % (36 of 40)	90.0 % (18 of 20)	90.0 % (18 of 20)
Methyl	93.3 % (28 of 30)	93.3 % (14 of 15)	93.3 % (14 of 15)

1. protein

GSSGSSGHLY YCSQCHYSSI TKNCLKRHVI QKHSNIL

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 296 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	protein	[U-13C; U-15N]	protein	0.72 mM
2	TRIS	[U-2H]		20 mM
3	sodium chloride	natural abundance		100 mM
4	DTT	[U-2H]		1 mM
5	sodium azide	natural abundance		0.02 %
6	zinc chloride	natural abundance		50 uM
7	H2O		solvent	90 %
8	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.42 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	4.792 ppm	internal	indirect	0.2514495
1H	water	protons	4.792 ppm	internal	direct	1.0
15N	water	protons	4.792 ppm	internal	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.42 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	4.792 ppm	internal	indirect	0.2514495
1H	water	protons	4.792 ppm	internal	direct	1.0
15N	water	protons	4.792 ppm	internal	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	4.792 ppm	internal	indirect	0.2514495
1H	water	protons	4.792 ppm	internal	direct	1.0
15N	water	protons	4.792 ppm	internal	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.16 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	4.792 ppm	internal	indirect	0.2514495
1H	water	protons	4.792 ppm	internal	direct	1.0
15N	water	protons	4.792 ppm	internal	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2RUY, Strand ID: A Detail

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Release date

2015-01-19

Citation

Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT
Tochio, N., Umehara, T., Kigawa, T., Yokoyama, S.
J. Struct. Funct. Genomics (2015), 16, 55-65, PubMed 25801860 , DOI 10.1007/s10969-015-9196-3 , Abstract

Related entities 1. protein, : 1 : 1 : 5 : 54 entities Detail

Interaction partners 1. protein, : 2 interactors Detail

Experiments performed 2 experiments Detail