BMRB: 11480

Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT

Authors

Tochio, N., Umehara, T., Kigawa, T., Yokoyama, S.

Assembly

protein

Entity

1. protein (polymer, Thiol state: all other bound), 37 monomers, 3997.344 Da Detail

GSSGSSGRSY SCPVCEKSFS EDRLIKSHIK TNHPEVS

2. ZINC ION (non-polymer), 65.409 Da

Total weight

4062.753 Da

Max. entity weight

3997.344 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS12:SG	2:ZN1:ZN
2	na	sing	1:CYS15:SG	2:ZN1:ZN
3	na	sing	1:HIS28:NE2	2:ZN1:ZN
4	na	sing	1:HIS33:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 86.5 %, Completeness: 88.6 %, Completeness (bb): 83.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	88.6 % (356 of 402)	88.2 % (186 of 211)	91.0 % (141 of 155)	80.6 % (29 of 36)
Backbone	83.5 % (182 of 218)	80.0 % (60 of 75)	87.0 % (94 of 108)	80.0 % (28 of 35)
Sidechain	94.0 % (205 of 218)	92.6 % (126 of 136)	96.3 % (78 of 81)	100.0 % (1 of 1)
Aromatic	100.0 % (26 of 26)	100.0 % (13 of 13)	100.0 % (13 of 13)
Methyl	100.0 % (22 of 22)	100.0 % (11 of 11)	100.0 % (11 of 11)

1. protein

GSSGSSGRSY SCPVCEKSFS EDRLIKSHIK TNHPEVS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 296 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	protein	[U-13C; U-15N]	protein	1.14 mM
2	TRIS	[U-2H]		20 mM
3	sodium chloride	natural abundance		100 mM
4	DTT	[U-2H]		1 mM
5	sodium azide	natural abundance		0.02 %
6	zinc chloride	natural abundance		50 uM
7	H2O		solvent	90 %
8	D2O		solvent	10 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2RV0, Strand ID: A Detail

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Release date

2015-01-19

Citation

Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT
Tochio, N., Umehara, T., Kigawa, T., Yokoyama, S.
J. Struct. Funct. Genomics (2015), 16, 55-65, PubMed 25801860 , DOI 10.1007/s10969-015-9196-3 , Abstract

Related entities 1. protein, : 1 : 1 : 5 : 137 entities Detail

Interaction partners 1. protein, : 2 interactors Detail

Experiments performed 2 experiments Detail