Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | na | sing | 1:CYS12:SG | 2:ZN1:ZN |
2 | na | sing | 1:CYS15:SG | 2:ZN1:ZN |
3 | na | sing | 1:HIS28:NE2 | 2:ZN1:ZN |
4 | na | sing | 1:HIS32:NE2 | 2:ZN1:ZN |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 86.0 % (337 of 392) | 86.8 % (178 of 205) | 88.1 % (133 of 151) | 72.2 % (26 of 36) |
Backbone | 78.2 % (169 of 216) | 75.6 % (59 of 78) | 82.4 % (84 of 102) | 72.2 % (26 of 36) |
Sidechain | 94.2 % (194 of 206) | 93.7 % (119 of 127) | 94.9 % (75 of 79) | |
Aromatic | 100.0 % (22 of 22) | 100.0 % (11 of 11) | 100.0 % (11 of 11) | |
Methyl | 100.0 % (24 of 24) | 100.0 % (12 of 12) | 100.0 % (12 of 12) |
1. protein
GSSGSSGKTH LCDMCGKKFK SKGTLKSHKL LHTADGSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 296 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | protein | [U-13C; U-15N] | protein | 0.11 mM |
2 | TRIS | [U-2H] | 20 mM | |
3 | sodium chloride | natural abundance | 100 mM | |
4 | DTT | [U-2H] | 1 mM | |
5 | sodium azide | natural abundance | 0.02 % | |
6 | zinc chloride | natural abundance | 50 uM | |
7 | H2O | solvent | 90 % | |
8 | D2O | solvent | 10 % |