BMRB: 11485

Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT

Authors

Tochio, N., Umehara, T., Kigawa, T., Yokoyama, S.

Assembly

protein

Entity

1. protein (polymer, Thiol state: all other bound), 35 monomers, 3705.234 Da Detail

GSSGSSGYVC ALCLKKFVSS IRLRSHIREV HGAAQ

2. ZINC ION (non-polymer), 65.409 Da

Total weight

3770.6428 Da

Max. entity weight

3705.234 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS10:SG	2:ZN1:ZN
2	na	sing	1:CYS13:SG	2:ZN1:ZN
3	na	sing	1:HIS26:NE2	2:ZN1:ZN
4	na	sing	1:HIS31:NE2	2:ZN1:ZN

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.6 %, Completeness: 90.3 %, Completeness (bb): 86.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.3 % (345 of 382)	89.8 % (177 of 197)	92.6 % (138 of 149)	83.3 % (30 of 36)
Backbone	86.7 % (182 of 210)	85.1 % (63 of 74)	89.1 % (90 of 101)	82.9 % (29 of 35)
Sidechain	94.1 % (191 of 203)	92.7 % (114 of 123)	96.2 % (76 of 79)	100.0 % (1 of 1)
Aromatic	100.0 % (26 of 26)	100.0 % (13 of 13)	100.0 % (13 of 13)
Methyl	100.0 % (38 of 38)	100.0 % (19 of 19)	100.0 % (19 of 19)

1. protein

GSSGSSGYVC ALCLKKFVSS IRLRSHIREV HGAAQ

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 296 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	protein	[U-13C; U-15N]	protein	1.10 mM
2	TRIS	[U-2H]		20 mM
3	sodium chloride	natural abundance		100 mM
4	DTT	[U-2H]		1 mM
5	sodium azide	natural abundance		0.02 %
6	zinc chloride	natural abundance		50 uM
7	H2O		solvent	90 %
8	D2O		solvent	10 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2RV5, Strand ID: A Detail

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Release date

2015-01-19

Citation

Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT
Tochio, N., Umehara, T., Kigawa, T., Yokoyama, S.
J. Struct. Funct. Genomics (2015), 16, 55-65, PubMed 25801860 , DOI 10.1007/s10969-015-9196-3 , Abstract

Related entities 1. protein, : 1 : 1 : 165 entities Detail

Experiments performed 2 experiments Detail