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Found 1 Document (0.866 seconds)

BMRB: 11487

2RV7

Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT

Authors

Tochio, N., Umehara, T., Kigawa, T., Yokoyama, S.

Assembly

protein

Entity

1. protein, entity 1 (polymer, Thiol state: free and other bound), 92 monomers, 10485.02 Da Detail

GSSGSSGKPY KCPQCSYASA IKANLNVHLR KHTGEKFACD YCSFTCLSKG HLKVHIERVH KKIKQHCRFC KKKYSDVKNL IKHIRDAHDP QD

2. ZINC ION (non-polymer), 65.409 × 3 Da

Total weight

10681.246 Da

Max. entity weight

10485.02 Da

Entity Connection

na 12 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS12:SG	2:ZN1:ZN
2	na	sing	1:CYS15:SG	2:ZN1:ZN
3	na	sing	1:HIS28:NE2	2:ZN1:ZN
4	na	sing	1:HIS32:NE2	2:ZN1:ZN
5	na	sing	1:CYS39:SG	2:ZN1:ZN
6	na	sing	1:CYS42:SG	2:ZN1:ZN
7	na	sing	1:HIS55:NE2	2:ZN1:ZN
8	na	sing	1:HIS60:NE2	2:ZN1:ZN
9	na	sing	1:CYS67:SG	2:ZN1:ZN
10	na	sing	1:CYS70:SG	2:ZN1:ZN
11	na	sing	1:HIS83:NE2	2:ZN1:ZN
12	na	sing	1:HIS88:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.6 %, Completeness: 92.4 %, Completeness (bb): 92.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.4 % (1014 of 1097)	91.0 % (528 of 580)	94.8 % (400 of 422)	90.5 % (86 of 95)
Backbone	92.1 % (503 of 546)	90.9 % (169 of 186)	93.7 % (254 of 271)	89.9 % (80 of 89)
Sidechain	93.1 % (594 of 638)	91.1 % (359 of 394)	96.2 % (229 of 238)	100.0 % (6 of 6)
Aromatic	97.9 % (92 of 94)	97.9 % (46 of 47)	97.9 % (46 of 47)
Methyl	100.0 % (70 of 70)	100.0 % (35 of 35)	100.0 % (35 of 35)

1. entity 1

GSSGSSGKPY KCPQCSYASA IKANLNVHLR KHTGEKFACD YCSFTCLSKG HLKVHIERVH KKIKQHCRFC KKKYSDVKNL IKHIRDAHDP QD

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 296 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	protein	[U-13C; U-15N]	protein	0.86 mM
2	TRIS	[U-2H]		20 mM
3	sodium chloride	natural abundance		100 mM
4	DTT	[U-2H]		1 mM
5	sodium azide	natural abundance		0.02 %
6	zinc chloride	natural abundance		50 uM
7	H2O		solvent	90 %
8	D2O		solvent	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.792 ppm	internal	indirect	0.2514495
¹H	water	protons	4.792 ppm	internal	direct	1.0
¹⁵N	water	protons	4.792 ppm	internal	indirect	0.1013291

Referencing offset: -0.27 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.792 ppm	internal	indirect	0.2514495
¹H	water	protons	4.792 ppm	internal	direct	1.0
¹⁵N	water	protons	4.792 ppm	internal	indirect	0.1013291

Referencing offset: -0.27 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.792 ppm	internal	indirect	0.2514495
¹H	water	protons	4.792 ppm	internal	direct	1.0
¹⁵N	water	protons	4.792 ppm	internal	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.792 ppm	internal	indirect	0.2514495
¹H	water	protons	4.792 ppm	internal	direct	1.0
¹⁵N	water	protons	4.792 ppm	internal	indirect	0.1013291

Referencing offset: 0.21 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2RSI, Strand ID: A Detail

Release date

2013-03-11

Citation

Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT
Tochio, N., Umehara, T., Kigawa, T., Yokoyama, S.
J. Struct. Funct. Genomics (2015), 16, 55-65, PubMed 25801860 , DOI 10.1007/s10969-015-9196-3 , Abstract

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Found 1 Document (0.866 seconds)

BMRB: 11487

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. protein, entity 1, : 1 : 1 : 1166 entities Detail

Interaction partners 1. protein, entity 1, : 2 interactors Detail

Experiments performed 2 experiments Detail

Instrument

Sample

Instrument

Sample