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Found 1 Document (2.137 seconds)

BMRB: 11488

2RV6

Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT

Authors

Tochio, N., Umehara, T., Kigawa, T., Yokoyama, S.

Assembly

protein

Entity

1. protein, entity 1 (polymer, Thiol state: free and other bound), 92 monomers, 10497.11 Da Detail

GSSGSSGKIF TCEYCNKVFK FKHSLQAHLR IHTNEKPYKC PQCSYASAIK ANLNVHLRKH TGEKFACDYC SFTCLSKGHL KVHIERVHKK IK

2. ZINC ION (non-polymer), 65.409 × 3 Da

Total weight

10693.337 Da

Max. entity weight

10497.11 Da

Entity Connection

na 12 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS12:SG	2:ZN1:ZN
2	na	sing	1:CYS15:SG	2:ZN1:ZN
3	na	sing	1:HIS28:NE2	2:ZN1:ZN
4	na	sing	1:HIS32:NE2	2:ZN1:ZN
5	na	sing	1:CYS40:SG	2:ZN1:ZN
6	na	sing	1:CYS43:SG	2:ZN1:ZN
7	na	sing	1:HIS56:NE2	2:ZN1:ZN
8	na	sing	1:HIS60:NE2	2:ZN1:ZN
9	na	sing	1:CYS67:SG	2:ZN1:ZN
10	na	sing	1:CYS70:SG	2:ZN1:ZN
11	na	sing	1:HIS83:NE2	2:ZN1:ZN
12	na	sing	1:HIS88:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.6 %, Completeness: 94.5 %, Completeness (bb): 93.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.5 % (1047 of 1108)	93.3 % (542 of 581)	97.0 % (418 of 431)	90.6 % (87 of 96)
Backbone	93.1 % (510 of 548)	90.9 % (170 of 187)	95.2 % (258 of 271)	91.1 % (82 of 90)
Sidechain	96.0 % (621 of 647)	94.4 % (372 of 394)	98.8 % (244 of 247)	83.3 % (5 of 6)
Aromatic	100.0 % (114 of 114)	100.0 % (57 of 57)	100.0 % (57 of 57)
Methyl	100.0 % (78 of 78)	100.0 % (39 of 39)	100.0 % (39 of 39)

1. entity 1

GSSGSSGKIF TCEYCNKVFK FKHSLQAHLR IHTNEKPYKC PQCSYASAIK ANLNVHLRKH TGEKFACDYC SFTCLSKGHL KVHIERVHKK IK

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 296 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	protein	[U-13C; U-15N]	protein	0.59 mM
2	TRIS	[U-2H]		20 mM
3	sodium chloride	natural abundance		100 mM
4	DTT	[U-2H]		1 mM
5	sodium azide	natural abundance		0.02 %
6	zinc chloride	natural abundance		50 uM
7	H2O		solvent	90 %
8	D2O		solvent	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.792 ppm	internal	indirect	0.2514495
¹H	water	protons	4.792 ppm	internal	direct	1.0
¹⁵N	water	protons	4.792 ppm	internal	indirect	0.1013291

Referencing offset: -0.22 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.792 ppm	internal	indirect	0.2514495
¹H	water	protons	4.792 ppm	internal	direct	1.0
¹⁵N	water	protons	4.792 ppm	internal	indirect	0.1013291

Referencing offset: -0.22 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.792 ppm	internal	indirect	0.2514495
¹H	water	protons	4.792 ppm	internal	direct	1.0
¹⁵N	water	protons	4.792 ppm	internal	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.792 ppm	internal	indirect	0.2514495
¹H	water	protons	4.792 ppm	internal	direct	1.0
¹⁵N	water	protons	4.792 ppm	internal	indirect	0.1013291

Referencing offset: 0.25 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2RSJ, Strand ID: A Detail

Release date

2013-03-11

Citation

Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT
Tochio, N., Umehara, T., Kigawa, T., Yokoyama, S.
J. Struct. Funct. Genomics (2015), 16, 55-65, PubMed 25801860 , DOI 10.1007/s10969-015-9196-3 , Abstract

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Found 1 Document (2.137 seconds)

BMRB: 11488

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. protein, entity 1, : 1 : 1 : 5 : 1386 entities Detail

Interaction partners 1. protein, entity 1, : 2 interactors Detail

Experiments performed 2 experiments Detail

Instrument

Sample

Instrument

Sample