BMRB: 11491

Solution structure and siRNA-mediated knockdown analysis of the mitochondrial disease-related protein C12orf65 (ICT2)

Authors

Enomoto, M., Tochio, N., Tomizawa, T., Koshiba, S., Guntert, P., Kigawa, T., Yokoyama, S., Nameki, N.

Assembly

the mitochondrial disease-related protein C12orf65

Entity

1. the mitochondrial disease-related protein C12orf65 (polymer, Thiol state: all free), 115 monomers, 12286.83 Da Detail

GSSGSSGKWG LRLQEKPALL FPGMAASTVQ VAGRKDYPAL LPLNESELEE QFVKGHGPGG QATNKTSNCV VLKHVPSGIV VKCHQTRSVD QNRKIARKVL QEKVDVFYNS GPSSG

Formula weight

12286.83 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.8 %, Completeness: 93.8 %, Completeness (bb): 91.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.8 % (1221 of 1302)	93.8 % (640 of 682)	95.0 % (474 of 499)	88.4 % (107 of 121)
Backbone	91.3 % (617 of 676)	90.7 % (214 of 236)	92.5 % (307 of 332)	88.9 % (96 of 108)
Sidechain	95.7 % (697 of 728)	94.8 % (423 of 446)	97.8 % (263 of 269)	84.6 % (11 of 13)
Aromatic	91.4 % (64 of 70)	88.6 % (31 of 35)	94.1 % (32 of 34)	100.0 % (1 of 1)
Methyl	100.0 % (118 of 118)	100.0 % (59 of 59)	100.0 % (59 of 59)

1. entity

GSSGSSGKWG LRLQEKPALL FPGMAASTVQ VAGRKDYPAL LPLNESELEE QFVKGHGPGG QATNKTSNCV VLKHVPSGIV VKCHQTRSVD QNRKIARKVL QEKVDVFYNS GPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 296 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-13C; U-15N]	protein	0.96 mM
2	TRIS	[U-2H]		20 mM
3	sodium chloride	natural abundance		100 mM
4	DTT	[U-2H]		1 mM
5	sodium azide	natural abundance		0.02 %
6	D2O	[U-2H]	solvent	10 %
7	H2O	natural abundance	solvent	90 %

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LACS Plot; CA

Referencing offset: -0.07 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	4.78 ppm	internal	indirect	0.2514495
1H	water	protons	4.78 ppm	internal	direct	1.0
15N	water	protons	4.78 ppm	internal	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.07 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	4.78 ppm	internal	indirect	0.2514495
1H	water	protons	4.78 ppm	internal	direct	1.0
15N	water	protons	4.78 ppm	internal	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.0 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	4.78 ppm	internal	indirect	0.2514495
1H	water	protons	4.78 ppm	internal	direct	1.0
15N	water	protons	4.78 ppm	internal	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.06 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	4.78 ppm	internal	indirect	0.2514495
1H	water	protons	4.78 ppm	internal	direct	1.0
15N	water	protons	4.78 ppm	internal	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2RSM, Strand ID: A Detail

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Release date

2012-08-05

Citation

Solution structure and siRNA-mediated knockdown analysis of the mitochondrial disease-related protein C12orf65
Kogure, H., Hikawa, Y., Hagihara, M., Tochio, N., Koshiba, S., Inoue, Y., Guntert, P., Kigawa, T., Yokoyama, S., Nameki, N.
Proteins (2012), 80, 2629-2642, PubMed 22821833 , DOI 10.1002/prot.24152 , Abstract

Entries sharing articles Swiss-Prot: 2 entries Detail

Related entities 1. the mitochondrial disease-related protein C12orf65, : 1 : 2 : 88 entities Detail

Experiments performed 2 experiments Detail