BMRBj - BMREntryMaster

Found 1 Document (1.183 seconds)

BMRB: 11508

2RSY

Solution structure of the SH2 domain of Csk in complex with a phosphopeptide from Cbp

Authors

Tanaka, H., Akagi, K., Oneyama, C., Tanaka, M., Sasaki, Y., Kanou, T., Lee, Y., Yokogawa, D., Debenecker, M., Nakagawa, A., Okada, M., Ikegami, T.

Assembly

Csk SH2:Cbp phosphopeptide complex

Entity

1. Csk SH2 (polymer, Thiol state: free and disulfide bound), 99 monomers, 11459.02 Da Detail

GPLGSMPWFH GKITREQAER LLYPPETGLF LVRESTNYPG DYTLCVSCEG KVEHYRIMYH ASKLSIDEEV YFENLMQLVE HYTTDADGLC TRLIKPKVM

2. Cbp phosphopeptide (polymer, Thiol state: not present), 38 monomers, 4258.525 Da Detail

GPLGSKRFSS LSYKSREEDP TLTEEEISAM XSSVNKPG

Total weight

15717.545 Da

Max. entity weight

11459.02 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS48:SG	1:CYS90:SG

Source organism

Rattus norvegicus

Exptl. method

solution NMR

Refine. method

simulated annealing, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.5 %, Completeness: 92.3 %, Completeness (bb): 97.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.3 % (1466 of 1588)	90.8 % (753 of 829)	92.8 % (581 of 626)	99.2 % (132 of 133)
Backbone	97.1 % (775 of 798)	96.7 % (264 of 273)	96.7 % (385 of 398)	99.2 % (126 of 127)
Sidechain	89.1 % (816 of 916)	87.9 % (489 of 556)	90.7 % (321 of 354)	100.0 % (6 of 6)
Aromatic	62.9 % (83 of 132)	65.2 % (43 of 66)	60.0 % (39 of 65)	100.0 % (1 of 1)
Methyl	100.0 % (130 of 130)	100.0 % (65 of 65)	100.0 % (65 of 65)

1. Csk SH2

GPLGSMPWFH GKITREQAER LLYPPETGLF LVRESTNYPG DYTLCVSCEG KVEHYRIMYH ASKLSIDEEV YFENLMQLVE HYTTDADGLC TRLIKPKVM

2. Cbp phosphopeptide

GPLGSKRFSS LSYKSREEDP TLTEEEISAM XSSVNKPG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	Csk SH2 domain	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		50 mM
4	Cbp phosphopeptide	natural abundance	protein	0.5 ~ 1.0 mM
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-99% 2H]	solvent	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
7	Csk SH2 domain	[U-15N]	protein	0.5 ~ 1.0 mM
8	sodium phosphate	natural abundance		20 mM
9	sodium chloride	natural abundance		50 mM
10	Cbp phosphopeptide	natural abundance	protein	0.5 ~ 1.0 mM
11	H2O	natural abundance		90 %
12	D2O	[U-99% 2H]		10 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
13	Cbp phosphopeptide	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
14	sodium phosphate	natural abundance		20 mM
15	sodium chloride	natural abundance		50 mM
16	Csk SH2 domain	natural abundance	protein	0.5 ~ 1.0 mM
17	H2O	natural abundance		90 %
18	D2O	[U-99% 2H]		10 %

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
19	Cbp phosphopeptide	[U-15N]	protein	0.5 ~ 1.0 mM
20	sodium phosphate	natural abundance		20 mM
21	sodium chloride	natural abundance		50 mM
22	Csk SH2 domain	natural abundance	protein	0.5 ~ 1.0 mM
23	H2O	natural abundance		90 %
24	D2O	[U-99% 2H]		10 %

Sample #5

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
25	Csk SH2 domain	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
26	sodium phosphate	natural abundance		20 mM
27	sodium chloride	natural abundance		50 mM
28	Cbp phosphopeptide	natural abundance	protein	0.5 ~ 1.0 mM
29	D2O	[U-99% 2H]		100 %

Sample #6

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
30	Cbp phosphopeptide	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
31	sodium phosphate	natural abundance		20 mM
32	sodium chloride	natural abundance		50 mM
33	Csk SH2 domain	natural abundance	protein	0.5 ~ 1.0 mM
34	D2O	[U-2H]		100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	direct	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	4.773 ppm	internal	direct	0.1013291

Referencing offset: -0.24 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	direct	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	4.773 ppm	internal	direct	0.1013291

Referencing offset: -0.24 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	direct	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	4.773 ppm	internal	direct	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	direct	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	4.773 ppm	internal	direct	0.1013291

Referencing offset: 0.2 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2RSY, Strand ID: A, B Detail

Release date

2013-04-07

Citation

A new SH2 domain binding mode of C-terminal Src kianse to Cbp/PAG"
Tanaka, H., Akagi, K., Oneyama, C., Tanaka, M., Sasaki, Y., Kanou, T., Lee, Y., Yokogawa, D., Debenecker, M., Nakagawa, A., Okada, M., Ikegami, T.
Not known

Related entities 1. Csk SH2, : 2 : 93 entities Detail

Related entities 2. Cbp phosphopeptide, : 1 : 4 : 3 entities Detail

Interaction partners 2. Cbp phosphopeptide, : 8 interactors Detail

More details for 8 interactors identified by 2 citations

Experiments performed 38 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz The cryo probe is equipped with to this spectrometer

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	Csk SH2 domain	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		50 mM
4	Cbp phosphopeptide	natural abundance	protein	0.5 ~ 1.0 mM
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-99% 2H]	solvent	10 %

2 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz The cryo probe is equipped with to this spectrometer

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
13	Cbp phosphopeptide	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
14	sodium phosphate	natural abundance		20 mM
15	sodium chloride	natural abundance		50 mM
16	Csk SH2 domain	natural abundance	protein	0.5 ~ 1.0 mM
17	H2O	natural abundance		90 %
18	D2O	[U-99% 2H]		10 %

3 3D HNCACB

Instrument

Bruker Avance - 800 MHz The cryo probe is equipped with to this spectrometer

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	Csk SH2 domain	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		50 mM
4	Cbp phosphopeptide	natural abundance	protein	0.5 ~ 1.0 mM
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-99% 2H]	solvent	10 %

4 3D HNCACB

Instrument

Bruker Avance - 800 MHz The cryo probe is equipped with to this spectrometer

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
13	Cbp phosphopeptide	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
14	sodium phosphate	natural abundance		20 mM
15	sodium chloride	natural abundance		50 mM
16	Csk SH2 domain	natural abundance	protein	0.5 ~ 1.0 mM
17	H2O	natural abundance		90 %
18	D2O	[U-99% 2H]		10 %

5 3D CBCA(CO)NH

Instrument

Bruker Avance - 800 MHz The cryo probe is equipped with to this spectrometer

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	Csk SH2 domain	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		50 mM
4	Cbp phosphopeptide	natural abundance	protein	0.5 ~ 1.0 mM
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-99% 2H]	solvent	10 %

6 3D CBCA(CO)NH

Instrument

Bruker Avance - 800 MHz The cryo probe is equipped with to this spectrometer

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
13	Cbp phosphopeptide	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
14	sodium phosphate	natural abundance		20 mM
15	sodium chloride	natural abundance		50 mM
16	Csk SH2 domain	natural abundance	protein	0.5 ~ 1.0 mM
17	H2O	natural abundance		90 %
18	D2O	[U-99% 2H]		10 %

7 3D HNCO

Instrument

Bruker Avance - 800 MHz The cryo probe is equipped with to this spectrometer

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	Csk SH2 domain	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		50 mM
4	Cbp phosphopeptide	natural abundance	protein	0.5 ~ 1.0 mM
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-99% 2H]	solvent	10 %

8 3D HNCO

Instrument

Bruker Avance - 800 MHz The cryo probe is equipped with to this spectrometer

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
13	Cbp phosphopeptide	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
14	sodium phosphate	natural abundance		20 mM
15	sodium chloride	natural abundance		50 mM
16	Csk SH2 domain	natural abundance	protein	0.5 ~ 1.0 mM
17	H2O	natural abundance		90 %
18	D2O	[U-99% 2H]		10 %

9 3D HN(CA)CO

Instrument

Bruker Avance - 800 MHz The cryo probe is equipped with to this spectrometer

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	Csk SH2 domain	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		50 mM
4	Cbp phosphopeptide	natural abundance	protein	0.5 ~ 1.0 mM
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-99% 2H]	solvent	10 %

10 3D HN(CA)CO

Instrument

Bruker Avance - 800 MHz The cryo probe is equipped with to this spectrometer

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
13	Cbp phosphopeptide	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
14	sodium phosphate	natural abundance		20 mM
15	sodium chloride	natural abundance		50 mM
16	Csk SH2 domain	natural abundance	protein	0.5 ~ 1.0 mM
17	H2O	natural abundance		90 %
18	D2O	[U-99% 2H]		10 %

11 3D HBHA(CO)NH

Instrument

Bruker Avance - 800 MHz The cryo probe is equipped with to this spectrometer

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	Csk SH2 domain	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		50 mM
4	Cbp phosphopeptide	natural abundance	protein	0.5 ~ 1.0 mM
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-99% 2H]	solvent	10 %

12 3D HBHA(CO)NH

Instrument

Bruker Avance - 800 MHz The cryo probe is equipped with to this spectrometer

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
13	Cbp phosphopeptide	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
14	sodium phosphate	natural abundance		20 mM
15	sodium chloride	natural abundance		50 mM
16	Csk SH2 domain	natural abundance	protein	0.5 ~ 1.0 mM
17	H2O	natural abundance		90 %
18	D2O	[U-99% 2H]		10 %

13 3D 1H-15N TOCSY

Instrument

Bruker Avance - 800 MHz The cryo probe is equipped with to this spectrometer

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
7	Csk SH2 domain	[U-15N]	protein	0.5 ~ 1.0 mM
8	sodium phosphate	natural abundance		20 mM
9	sodium chloride	natural abundance		50 mM
10	Cbp phosphopeptide	natural abundance	protein	0.5 ~ 1.0 mM
11	H2O	natural abundance		90 %
12	D2O	[U-99% 2H]		10 %

14 3D 1H-15N TOCSY

Instrument

Bruker Avance - 800 MHz The cryo probe is equipped with to this spectrometer

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
19	Cbp phosphopeptide	[U-15N]	protein	0.5 ~ 1.0 mM
20	sodium phosphate	natural abundance		20 mM
21	sodium chloride	natural abundance		50 mM
22	Csk SH2 domain	natural abundance	protein	0.5 ~ 1.0 mM
23	H2O	natural abundance		90 %
24	D2O	[U-99% 2H]		10 %

15 2D 1H-13C HSQC

Instrument

Bruker Avance - 800 MHz The cryo probe is equipped with to this spectrometer

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
25	Csk SH2 domain	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
26	sodium phosphate	natural abundance		20 mM
27	sodium chloride	natural abundance		50 mM
28	Cbp phosphopeptide	natural abundance	protein	0.5 ~ 1.0 mM
29	D2O	[U-99% 2H]		100 %

16 2D 1H-13C HSQC

Instrument

Bruker Avance - 800 MHz The cryo probe is equipped with to this spectrometer

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
30	Cbp phosphopeptide	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
31	sodium phosphate	natural abundance		20 mM
32	sodium chloride	natural abundance		50 mM
33	Csk SH2 domain	natural abundance	protein	0.5 ~ 1.0 mM
34	D2O	[U-2H]		100 %

17 2D 1H-13C HSQC aromatic

Instrument

Bruker DRX - 800 MHz The cryo probe is equipped with to this spectrometer

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
25	Csk SH2 domain	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
26	sodium phosphate	natural abundance		20 mM
27	sodium chloride	natural abundance		50 mM
28	Cbp phosphopeptide	natural abundance	protein	0.5 ~ 1.0 mM
29	D2O	[U-99% 2H]		100 %

18 2D 1H-13C HSQC aromatic

Instrument

Bruker DRX - 800 MHz The cryo probe is equipped with to this spectrometer

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
30	Cbp phosphopeptide	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
31	sodium phosphate	natural abundance		20 mM
32	sodium chloride	natural abundance		50 mM
33	Csk SH2 domain	natural abundance	protein	0.5 ~ 1.0 mM
34	D2O	[U-2H]		100 %

19 3D 1H-13C NOESY aromatic

Instrument

Bruker DRX - 800 MHz The cryo probe is equipped with to this spectrometer

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
25	Csk SH2 domain	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
26	sodium phosphate	natural abundance		20 mM
27	sodium chloride	natural abundance		50 mM
28	Cbp phosphopeptide	natural abundance	protein	0.5 ~ 1.0 mM
29	D2O	[U-99% 2H]		100 %

20 3D 1H-13C NOESY aromatic

Instrument

Bruker DRX - 800 MHz The cryo probe is equipped with to this spectrometer

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
30	Cbp phosphopeptide	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
31	sodium phosphate	natural abundance		20 mM
32	sodium chloride	natural abundance		50 mM
33	Csk SH2 domain	natural abundance	protein	0.5 ~ 1.0 mM
34	D2O	[U-2H]		100 %

21 2D DQF-COSY

Instrument

Bruker DRX - 800 MHz The cryo probe is equipped with to this spectrometer

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
25	Csk SH2 domain	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
26	sodium phosphate	natural abundance		20 mM
27	sodium chloride	natural abundance		50 mM
28	Cbp phosphopeptide	natural abundance	protein	0.5 ~ 1.0 mM
29	D2O	[U-99% 2H]		100 %

22 3D C(CO)NH

Instrument

Bruker Avance - 800 MHz The cryo probe is equipped with to this spectrometer

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	Csk SH2 domain	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		50 mM
4	Cbp phosphopeptide	natural abundance	protein	0.5 ~ 1.0 mM
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-99% 2H]	solvent	10 %

23 3D C(CO)NH

Instrument

Bruker Avance - 800 MHz The cryo probe is equipped with to this spectrometer

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
13	Cbp phosphopeptide	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
14	sodium phosphate	natural abundance		20 mM
15	sodium chloride	natural abundance		50 mM
16	Csk SH2 domain	natural abundance	protein	0.5 ~ 1.0 mM
17	H2O	natural abundance		90 %
18	D2O	[U-99% 2H]		10 %

24 3D H(CCO)NH

Instrument

Bruker Avance - 800 MHz The cryo probe is equipped with to this spectrometer

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	Csk SH2 domain	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		50 mM
4	Cbp phosphopeptide	natural abundance	protein	0.5 ~ 1.0 mM
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-99% 2H]	solvent	10 %

25 3D H(CCO)NH

Instrument

Bruker Avance - 800 MHz The cryo probe is equipped with to this spectrometer

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
13	Cbp phosphopeptide	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
14	sodium phosphate	natural abundance		20 mM
15	sodium chloride	natural abundance		50 mM
16	Csk SH2 domain	natural abundance	protein	0.5 ~ 1.0 mM
17	H2O	natural abundance		90 %
18	D2O	[U-99% 2H]		10 %

26 3D HCCH-TOCSY

Instrument

Bruker Avance - 800 MHz The cryo probe is equipped with to this spectrometer

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
25	Csk SH2 domain	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
26	sodium phosphate	natural abundance		20 mM
27	sodium chloride	natural abundance		50 mM
28	Cbp phosphopeptide	natural abundance	protein	0.5 ~ 1.0 mM
29	D2O	[U-99% 2H]		100 %

27 3D HCCH-TOCSY

Instrument

Bruker Avance - 800 MHz The cryo probe is equipped with to this spectrometer

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
30	Cbp phosphopeptide	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
31	sodium phosphate	natural abundance		20 mM
32	sodium chloride	natural abundance		50 mM
33	Csk SH2 domain	natural abundance	protein	0.5 ~ 1.0 mM
34	D2O	[U-2H]		100 %

28 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz The cryo probe is equipped with to this spectrometer

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
7	Csk SH2 domain	[U-15N]	protein	0.5 ~ 1.0 mM
8	sodium phosphate	natural abundance		20 mM
9	sodium chloride	natural abundance		50 mM
10	Cbp phosphopeptide	natural abundance	protein	0.5 ~ 1.0 mM
11	H2O	natural abundance		90 %
12	D2O	[U-99% 2H]		10 %

29 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz The cryo probe is equipped with to this spectrometer

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
19	Cbp phosphopeptide	[U-15N]	protein	0.5 ~ 1.0 mM
20	sodium phosphate	natural abundance		20 mM
21	sodium chloride	natural abundance		50 mM
22	Csk SH2 domain	natural abundance	protein	0.5 ~ 1.0 mM
23	H2O	natural abundance		90 %
24	D2O	[U-99% 2H]		10 %

30 3D 1H-13C NOESY

Instrument

Bruker Avance - 800 MHz The cryo probe is equipped with to this spectrometer

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
25	Csk SH2 domain	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
26	sodium phosphate	natural abundance		20 mM
27	sodium chloride	natural abundance		50 mM
28	Cbp phosphopeptide	natural abundance	protein	0.5 ~ 1.0 mM
29	D2O	[U-99% 2H]		100 %

31 3D 1H-13C NOESY

Instrument

Bruker Avance - 800 MHz The cryo probe is equipped with to this spectrometer

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
30	Cbp phosphopeptide	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
31	sodium phosphate	natural abundance		20 mM
32	sodium chloride	natural abundance		50 mM
33	Csk SH2 domain	natural abundance	protein	0.5 ~ 1.0 mM
34	D2O	[U-2H]		100 %

32 2D CBHD

Instrument

Bruker Avance - 800 MHz The cryo probe is equipped with to this spectrometer

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
25	Csk SH2 domain	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
26	sodium phosphate	natural abundance		20 mM
27	sodium chloride	natural abundance		50 mM
28	Cbp phosphopeptide	natural abundance	protein	0.5 ~ 1.0 mM
29	D2O	[U-99% 2H]		100 %

33 2D CBHD

Instrument

Bruker Avance - 800 MHz The cryo probe is equipped with to this spectrometer

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
30	Cbp phosphopeptide	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
31	sodium phosphate	natural abundance		20 mM
32	sodium chloride	natural abundance		50 mM
33	Csk SH2 domain	natural abundance	protein	0.5 ~ 1.0 mM
34	D2O	[U-2H]		100 %

34 2D CBHE

Instrument

Bruker Avance - 800 MHz The cryo probe is equipped with to this spectrometer

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
25	Csk SH2 domain	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
26	sodium phosphate	natural abundance		20 mM
27	sodium chloride	natural abundance		50 mM
28	Cbp phosphopeptide	natural abundance	protein	0.5 ~ 1.0 mM
29	D2O	[U-99% 2H]		100 %

35 2D CBHE

Instrument

Bruker Avance - 800 MHz The cryo probe is equipped with to this spectrometer

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
30	Cbp phosphopeptide	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
31	sodium phosphate	natural abundance		20 mM
32	sodium chloride	natural abundance		50 mM
33	Csk SH2 domain	natural abundance	protein	0.5 ~ 1.0 mM
34	D2O	[U-2H]		100 %

36 3D 13C-filtered/13C-edited NOESY

Instrument

Bruker DRX - 800 MHz The cryo probe is equipped with to this spectrometer

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
25	Csk SH2 domain	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
26	sodium phosphate	natural abundance		20 mM
27	sodium chloride	natural abundance		50 mM
28	Cbp phosphopeptide	natural abundance	protein	0.5 ~ 1.0 mM
29	D2O	[U-99% 2H]		100 %

37 3D 13C-filtered/13C-edited NOESY

Instrument

Bruker DRX - 800 MHz The cryo probe is equipped with to this spectrometer

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
30	Cbp phosphopeptide	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
31	sodium phosphate	natural abundance		20 mM
32	sodium chloride	natural abundance		50 mM
33	Csk SH2 domain	natural abundance	protein	0.5 ~ 1.0 mM
34	D2O	[U-2H]		100 %

38 3D 13C,15N-filtered/15N-edited NOESY

Instrument

Bruker DRX - 800 MHz The cryo probe is equipped with to this spectrometer

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
13	Cbp phosphopeptide	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
14	sodium phosphate	natural abundance		20 mM
15	sodium chloride	natural abundance		50 mM
16	Csk SH2 domain	natural abundance	protein	0.5 ~ 1.0 mM
17	H2O	natural abundance		90 %
18	D2O	[U-99% 2H]		10 %

nullKeywords Cbp, Csk, nmr, SH2 domain, solution structure

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (1.183 seconds)

BMRB: 11508

Sample #1

Sample #2

Sample #3

Sample #4

Sample #5

Sample #6

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Csk SH2, : 2 : 93 entities Detail

Related entities 2. Cbp phosphopeptide, : 1 : 4 : 3 entities Detail

Interaction partners 2. Cbp phosphopeptide, : 8 interactors Detail

Experiments performed 38 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument