BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	88.8 % (1010 of 1138)	86.0 % (511 of 594)	92.5 % (406 of 439)	88.6 % (93 of 105)
Backbone	97.7 % (559 of 572)	95.4 % (186 of 195)	98.6 % (281 of 285)	100.0 % (92 of 92)
Sidechain	82.5 % (542 of 657)	81.5 % (325 of 399)	88.2 % (216 of 245)	7.7 % (1 of 13)
Aromatic	34.7 % (25 of 72)	38.9 % (14 of 36)	28.6 % (10 of 35)	100.0 % (1 of 1)
Methyl	97.5 % (119 of 122)	95.1 % (58 of 61)	100.0 % (61 of 61)

1. Nrd1 RRM2

NSASNSSVLL AVQQSGACRN VFLGNLPNGI TEDEIREDLE PFGPIDQIKI VTERNIAFVH FLNIAAAIKA VQELPLNPKW SKRRIYYGRD RCAVGLK

Show sample condition

Sample #1

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	potassium phosphate	natural abundance		50 mM
2	sodium chloride	natural abundance		100 mM
3	TCEP	natural abundance		1 mM
4	EDTA	natural abundance		0.1 mM
5	Nrd1 RRM2	[U-99% 13C; U-99% 15N]	protein	1.0 mM
6	H2O		solvent	93 %
7	D2O		solvent	7 %

Sample #2

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
8	potassium phosphate	natural abundance		50 mM
9	sodium chloride	natural abundance		100 mM
10	TCEP	natural abundance		1 mM
11	EDTA	natural abundance		0.1 mM
12	Nrd1 RRM2	[U-99% 15N]	protein	1.2 mM
13	H2O		solvent	93 %
14	D2O		solvent	7 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
15	potassium phosphate	natural abundance		50 mM
16	sodium chloride	natural abundance		100 mM
17	TCEP	natural abundance		1 mM
18	EDTA	natural abundance		0.1 mM
19	Nrd1 RRM2	[U-99% 13C; U-99% 15N]	protein	1.0 mM
20	D2O		solvent	100 %

Sample #4

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
21	potassium phosphate	natural abundance		50 mM
22	sodium chloride	natural abundance		100 mM
23	TCEP	natural abundance		1 mM
24	EDTA	natural abundance		0.1 mM
25	Nrd1 RRM2	[U-10% 13C; U-99% 15N]	protein	0.35 mM
26	H2O		solvent	93 %
27	D2O		solvent	7 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.23 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.23 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.39 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2RT3, Strand ID: A Detail

Release date

2014-04-13

Citation

Structure of the second RRM domain of Nrd1, a fission yeast MAPK target RNA binding protein, and implication for its RNA recognition and regulation
Kobayashi, A., Kanaba, T., Satoh, R., Fujiwara, T., Ito, Y., Sugiura, R., Mishima, M.
Biochem. Biophys. Res. Commun. (2013), 437, 12-17, PubMed 23770370 , DOI 10.1016/j.bbrc.2013.06.008 , Abstract

Related entities 1. the second RRM domain of Nrd1, : 1 : 2 : 37 entities Detail

Interaction partners 1. the second RRM domain of Nrd1, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
8	potassium phosphate	natural abundance		50 mM
9	sodium chloride	natural abundance		100 mM
10	TCEP	natural abundance		1 mM
11	EDTA	natural abundance		0.1 mM
12	Nrd1 RRM2	[U-99% 15N]	protein	1.2 mM
13	H2O		solvent	93 %
14	D2O		solvent	7 %

2 3D HNCACB

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	potassium phosphate	natural abundance		50 mM
2	sodium chloride	natural abundance		100 mM
3	TCEP	natural abundance		1 mM
4	EDTA	natural abundance		0.1 mM
5	Nrd1 RRM2	[U-99% 13C; U-99% 15N]	protein	1.0 mM
6	H2O		solvent	93 %
7	D2O		solvent	7 %

3 3D CBCA(CO)NH

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	potassium phosphate	natural abundance		50 mM
2	sodium chloride	natural abundance		100 mM
3	TCEP	natural abundance		1 mM
4	EDTA	natural abundance		0.1 mM
5	Nrd1 RRM2	[U-99% 13C; U-99% 15N]	protein	1.0 mM
6	H2O		solvent	93 %
7	D2O		solvent	7 %

4 3D HN(CA)CO

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	potassium phosphate	natural abundance		50 mM
2	sodium chloride	natural abundance		100 mM
3	TCEP	natural abundance		1 mM
4	EDTA	natural abundance		0.1 mM
5	Nrd1 RRM2	[U-99% 13C; U-99% 15N]	protein	1.0 mM
6	H2O		solvent	93 %
7	D2O		solvent	7 %

5 3D HNCO

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	potassium phosphate	natural abundance		50 mM
2	sodium chloride	natural abundance		100 mM
3	TCEP	natural abundance		1 mM
4	EDTA	natural abundance		0.1 mM
5	Nrd1 RRM2	[U-99% 13C; U-99% 15N]	protein	1.0 mM
6	H2O		solvent	93 %
7	D2O		solvent	7 %

6 3D H(CCO)NH

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	potassium phosphate	natural abundance		50 mM
2	sodium chloride	natural abundance		100 mM
3	TCEP	natural abundance		1 mM
4	EDTA	natural abundance		0.1 mM
5	Nrd1 RRM2	[U-99% 13C; U-99% 15N]	protein	1.0 mM
6	H2O		solvent	93 %
7	D2O		solvent	7 %

7 3D C(CO)NH

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	potassium phosphate	natural abundance		50 mM
2	sodium chloride	natural abundance		100 mM
3	TCEP	natural abundance		1 mM
4	EDTA	natural abundance		0.1 mM
5	Nrd1 RRM2	[U-99% 13C; U-99% 15N]	protein	1.0 mM
6	H2O		solvent	93 %
7	D2O		solvent	7 %

8 4D H(CCO)NH

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	potassium phosphate	natural abundance		50 mM
2	sodium chloride	natural abundance		100 mM
3	TCEP	natural abundance		1 mM
4	EDTA	natural abundance		0.1 mM
5	Nrd1 RRM2	[U-99% 13C; U-99% 15N]	protein	1.0 mM
6	H2O		solvent	93 %
7	D2O		solvent	7 %

9 3D 1H-13C NOESY

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
15	potassium phosphate	natural abundance		50 mM
16	sodium chloride	natural abundance		100 mM
17	TCEP	natural abundance		1 mM
18	EDTA	natural abundance		0.1 mM
19	Nrd1 RRM2	[U-99% 13C; U-99% 15N]	protein	1.0 mM
20	D2O		solvent	100 %

10 3D 1H-15N NOESY

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
8	potassium phosphate	natural abundance		50 mM
9	sodium chloride	natural abundance		100 mM
10	TCEP	natural abundance		1 mM
11	EDTA	natural abundance		0.1 mM
12	Nrd1 RRM2	[U-99% 15N]	protein	1.2 mM
13	H2O		solvent	93 %
14	D2O		solvent	7 %

11 3D HNHB

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
8	potassium phosphate	natural abundance		50 mM
9	sodium chloride	natural abundance		100 mM
10	TCEP	natural abundance		1 mM
11	EDTA	natural abundance		0.1 mM
12	Nrd1 RRM2	[U-99% 15N]	protein	1.2 mM
13	H2O		solvent	93 %
14	D2O		solvent	7 %

12 3D HA(CA)HB

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
15	potassium phosphate	natural abundance		50 mM
16	sodium chloride	natural abundance		100 mM
17	TCEP	natural abundance		1 mM
18	EDTA	natural abundance		0.1 mM
19	Nrd1 RRM2	[U-99% 13C; U-99% 15N]	protein	1.0 mM
20	D2O		solvent	100 %

13 2D 1H-13C CT-HSQC

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
21	potassium phosphate	natural abundance		50 mM
22	sodium chloride	natural abundance		100 mM
23	TCEP	natural abundance		1 mM
24	EDTA	natural abundance		0.1 mM
25	Nrd1 RRM2	[U-10% 13C; U-99% 15N]	protein	0.35 mM
26	H2O		solvent	93 %
27	D2O		solvent	7 %

nullKeywords Nrd1, RNA-binding, Solution structure

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Found 1 Document (0.905 seconds)

BMRB: 11523

Sample #1

Sample #2

Sample #3

Sample #4

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. the second RRM domain of Nrd1, : 1 : 2 : 37 entities Detail

Interaction partners 1. the second RRM domain of Nrd1, : 1 interactors Detail

Experiments performed 13 experiments Detail

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