BMRB: 11524

NMR Structure of designed protein, AF.2A1, (Ensembles)

Authors

Watanabe, H., Yamasaki, K., Honda, S.

Assembly

AF.2A1

Entity

1. AF.2A1 (polymer), 25 monomers, 2713.918 Da Detail

GVVRQWSGYD PRTGTWRSSI AYGGG

Formula weight

2713.918 Da

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.9 %, Completeness (bb): 98.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	97.9 % (137 of 140)	97.9 % (137 of 140)
Backbone	98.2 % (54 of 55)	98.2 % (54 of 55)
Sidechain	97.6 % (83 of 85)	97.6 % (83 of 85)
Aromatic	100.0 % (20 of 20)	100.0 % (20 of 20)
Methyl	100.0 % (9 of 9)	100.0 % (9 of 9)

1. entity

GVVRQWSGYD PRTGTWRSSI AYGGG

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 283 K, pH 4.5

#	Name	Isotope labeling	Type	Concentration
1	entity	natural abundance	protein	1.8 mM
2	sodium acetate-d4	[U-100% 2H]		10 mM
3	D2O		solvent	5 %
4	H2O		solvent	95 %

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Calculated from 20 models in PDB: 2RT4, Strand ID: A Detail

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Release date

2014-01-01

Citation

Tracing primordial protein evolution through structurally guided stepwise segment elongation
Watanabe, H., Yamasaki, K., Honda, S.
J. Biol. Chem. (2014), 289, 3394-3404, PubMed 24356963 , DOI 10.1074/jbc.M113.530592 , Abstract

Related entities 1. AF.2A1, : 1 entities Detail

Experiments performed 3 experiments Detail