BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	95.5 % (1085 of 1136)	95.4 % (559 of 586)	95.5 % (420 of 440)	96.4 % (106 of 110)
Backbone	96.6 % (564 of 584)	96.4 % (189 of 196)	96.9 % (283 of 292)	95.8 % (92 of 96)
Sidechain	94.8 % (614 of 648)	94.9 % (370 of 390)	94.3 % (230 of 244)	100.0 % (14 of 14)
Aromatic	84.6 % (66 of 78)	84.6 % (33 of 39)	83.3 % (30 of 36)	100.0 % (3 of 3)
Methyl	97.8 % (131 of 134)	98.5 % (66 of 67)	97.0 % (65 of 67)

1. PriC N-terminal domain

MKTALLLEKL EGQLATLRQR CAPVSQFATL SARFDRHLFQ TRATTLQACL DEAGDNLAAL RHAVEQQQLP QVAWLAEHLA AQLEAIAREA SAWSLREW

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	PriC N-terminal domain	[U-99% 13C; U-99% 15N]	protein	0.5 mM
2	sodium chloride	natural abundance		150 mM
3	H2O		solvent	90 %
4	D2O		solvent	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.32 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2RT6, Strand ID: A Detail

Release date

2013-08-04

Citation

Solution structure of the N-terminal domain of a replication restart primosome factor, PriC, in Escherichia coli
Aramaki, T., Abe, Y., Katayama, T., Ueda, T.
Protein Sci. (2013), 22, 1279-1286, PubMed 23868391 , DOI 10.1002/pro.2314 , Abstract

Related entities 1. PriC N-temrinal domain, : 1 : 2 : 3 entities Detail

Interaction partners 1. PriC N-temrinal domain, : 20 interactors Detail

More details for 20 interactors identified by 3 citations

Experiments performed 18 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	PriC N-terminal domain	[U-99% 13C; U-99% 15N]	protein	0.5 mM
2	sodium chloride	natural abundance		150 mM
3	H2O		solvent	90 %
4	D2O		solvent	10 %

2 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
5	PriC N-terminal domain	[U-98% 15N]	protein	0.2 mM
6	sodium chloride	natural abundance		150 mM
7	D2O		solvent	100 %

3 2D 1H-13C HSQC aliphatic

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	PriC N-terminal domain	[U-99% 13C; U-99% 15N]	protein	0.5 mM
2	sodium chloride	natural abundance		150 mM
3	H2O		solvent	90 %
4	D2O		solvent	10 %

4 2D 1H-13C HSQC aromatic

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	PriC N-terminal domain	[U-99% 13C; U-99% 15N]	protein	0.5 mM
2	sodium chloride	natural abundance		150 mM
3	H2O		solvent	90 %
4	D2O		solvent	10 %

5 3D CBCA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	PriC N-terminal domain	[U-99% 13C; U-99% 15N]	protein	0.5 mM
2	sodium chloride	natural abundance		150 mM
3	H2O		solvent	90 %
4	D2O		solvent	10 %

6 3D C(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	PriC N-terminal domain	[U-99% 13C; U-99% 15N]	protein	0.5 mM
2	sodium chloride	natural abundance		150 mM
3	H2O		solvent	90 %
4	D2O		solvent	10 %

7 3D HNCO

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	PriC N-terminal domain	[U-99% 13C; U-99% 15N]	protein	0.5 mM
2	sodium chloride	natural abundance		150 mM
3	H2O		solvent	90 %
4	D2O		solvent	10 %

8 3D HNCA

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	PriC N-terminal domain	[U-99% 13C; U-99% 15N]	protein	0.5 mM
2	sodium chloride	natural abundance		150 mM
3	H2O		solvent	90 %
4	D2O		solvent	10 %

9 3D HNCACB

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	PriC N-terminal domain	[U-99% 13C; U-99% 15N]	protein	0.5 mM
2	sodium chloride	natural abundance		150 mM
3	H2O		solvent	90 %
4	D2O		solvent	10 %

10 3D HBHA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	PriC N-terminal domain	[U-99% 13C; U-99% 15N]	protein	0.5 mM
2	sodium chloride	natural abundance		150 mM
3	H2O		solvent	90 %
4	D2O		solvent	10 %

11 3D HN(CO)CA

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	PriC N-terminal domain	[U-99% 13C; U-99% 15N]	protein	0.5 mM
2	sodium chloride	natural abundance		150 mM
3	H2O		solvent	90 %
4	D2O		solvent	10 %

12 3D H(CCO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	PriC N-terminal domain	[U-99% 13C; U-99% 15N]	protein	0.5 mM
2	sodium chloride	natural abundance		150 mM
3	H2O		solvent	90 %
4	D2O		solvent	10 %

13 3D HCCH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	PriC N-terminal domain	[U-99% 13C; U-99% 15N]	protein	0.5 mM
2	sodium chloride	natural abundance		150 mM
3	H2O		solvent	90 %
4	D2O		solvent	10 %

14 3D 1H-15N NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	PriC N-terminal domain	[U-99% 13C; U-99% 15N]	protein	0.5 mM
2	sodium chloride	natural abundance		150 mM
3	H2O		solvent	90 %
4	D2O		solvent	10 %

15 3D 1H-15N TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	PriC N-terminal domain	[U-99% 13C; U-99% 15N]	protein	0.5 mM
2	sodium chloride	natural abundance		150 mM
3	H2O		solvent	90 %
4	D2O		solvent	10 %

16 3D 1H-13C NOESY aliphatic

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	PriC N-terminal domain	[U-99% 13C; U-99% 15N]	protein	0.5 mM
2	sodium chloride	natural abundance		150 mM
3	H2O		solvent	90 %
4	D2O		solvent	10 %

17 3D 1H-13C NOESY aromatic

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	PriC N-terminal domain	[U-99% 13C; U-99% 15N]	protein	0.5 mM
2	sodium chloride	natural abundance		150 mM
3	H2O		solvent	90 %
4	D2O		solvent	10 %

18 3D HCCH-COSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	PriC N-terminal domain	[U-99% 13C; U-99% 15N]	protein	0.5 mM
2	sodium chloride	natural abundance		150 mM
3	H2O		solvent	90 %
4	D2O		solvent	10 %

NMR combined restraints 5 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_11525_2rt6.nef
Input source #2: Coordindates	2rt6.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10--------20--------30--------40--------50--------60--------70--------80--------90--------
MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRATTLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREW
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRATTLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREW

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	98	0	0	100.0

Content subtype: combined_11525_2rt6.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	1097		99.0 (chain: A, length: 98)
1	Distance restraints	CNS/XPLOR_distance_constraints_4	OK	1310 (1)	noe	99.0 (chain: A, length: 98)
2	Distance restraints	CNS/XPLOR_distance_constraints_3	OK	92 (1)	hbond	60.2 (chain: A, length: 98)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_2	OK	174 (174)	.	94.9 (chain: A, length: 98)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 98, Sequence coverage: 99.0%
- Total number of assignments
  - ¹H chemical shifts: 562
  - ¹³C chemical shifts: 423
  - ¹⁵N chemical shifts: 112
- Completeness of assignments
  - Entity_assemble_ID: A
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	586	562	95.9
¹³C chemical shifts	440	423	96.1
¹⁵N chemical shifts	118	112	94.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	196	191	97.4
¹³C chemical shifts	196	192	98.0
¹⁵N chemical shifts	96	93	96.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	390	371	95.1
¹³C chemical shifts	244	231	94.7
¹⁵N chemical shifts	22	19	86.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	68	67	98.5
¹³C chemical shifts	68	66	97.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	39	33	84.6
¹³C chemical shifts	36	30	83.3
¹⁵N chemical shifts	3	3	100.0

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_4
- Status: OK
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 98, Sequence coverage: 99.0%
- Total number of restraints: 1298
- Weight of restraints: 1.0 (1298)
- Potential type of restraints: square-well-parabolic (1298)
- Range of distance target values: 2.45, 4.15 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map
- Saveframe: CNS/XPLOR_distance_constraints_3
  - Status: OK
  - Experiment type: hbond
  - Sequence coverage of experimental data
    - Entity_assembly_ID: A, Chain length: 98, Sequence coverage: 60.2%
  - Total number of restraints: 92
  - Weight of restraints: 1.0 (92)
  - Potential type of restraints: square-well-parabolic (92)
  - Range of distance target values: 2.0, 2.95 (Å)
  - Histogram of distance target values
  - Distance restraints per residue
    - Chain_ID: A
  - Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_2
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 98, Sequence coverage: 94.9%
- Total number of restraints: 174
- Total number of restraints per polymer type: 174
- Weight of restraints: 1.0 (174)
- Potential type of restraints: square-well-parabolic (174)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A

Keywords PriC, Primosome, Protein structure, Replication restart

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (0.774 seconds)

BMRB: 11525

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. PriC N-temrinal domain, : 1 : 2 : 3 entities Detail

Interaction partners 1. PriC N-temrinal domain, : 20 interactors Detail

Experiments performed 18 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

NMR combined restraints 5 contents Detail