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BMRB: 11529


11529
2RT9
Solution structure of a regulatory domain of meiosis inhibitor
Authors
Shoji, S., Muto, Y., Ikeda, M., He, F., Tsuda, K., Ohsawa, N., Akasaka, R., Terada, T., Wakiyama, M., Shirouzu, M., Yokoyama, S.
Assembly
a regulatory domain of meiosis inhibitor
Entity
1. a regulatory domain of meiosis inhibitor, entity 1 (polymer), 59 monomers, 6339.192 Da Detail

GSSGSSGTDE ALKPCPRCQS PAKYQPHKKR GLCSRLACGF DFCVLCLCAY HGSEDCRRG


2. ZINC ION (non-polymer), 65.409 × 2 Da
Total weight
6470.01 Da
Max. entity weight
6339.192 Da
Source organism
Mus musculus
Exptl. method
solution NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 93.2 %, Completeness: 93.6 %, Completeness (bb): 90.8 % Detail
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Polymer type: polypeptide(L)

Total1H13C15N
All93.6 % (596 of 637)94.3 % (317 of 336)93.0 % (227 of 244)91.2 % (52 of 57)
Backbone90.8 % (314 of 346)91.7 % (111 of 121)90.0 % (153 of 170)90.9 % (50 of 55)
Sidechain96.2 % (330 of 343)95.8 % (206 of 215)96.8 % (122 of 126)100.0 % (2 of 2)
Aromatic100.0 % (44 of 44)100.0 % (22 of 22)100.0 % (22 of 22)
Methyl100.0 % (34 of 34)100.0 % (17 of 17)100.0 % (17 of 17)

1. entity 1

GSSGSSGTDE ALKPCPRCQS PAKYQPHKKR GLCSRLACGF DFCVLCLCAY HGSEDCRRG

Show sample condition
Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 20mM dTris-HCl(pH 6.5), 100mM NaCl, 1mM d-DTT, 50uM ZnCl2, 0.02% NaN3


#NameIsotope labelingTypeConcentration
1entity_1[U-100% 13C; U-100% 15N]protein0.47 mM
2H2Osolvent90 %
3D2Osolvent10 %
4dTris-HCl[U-2H]buffer20 mM
5NaClnatural abundancesalt100 mM
6d-DTT[U-2H]1 mM
7ZnCl2natural abundance50 uM
8NaN3natural abundance0.02 %

LACS Plot; CA
Referencing offset: -0.18 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.18 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: 0.02 ppm, Outliers: 2 Detail
LACS Plot; CO
Referencing offset: 3.15 ppm, Outliers: 2 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2RT9, Strand ID: A Detail

Release date
2014-07-13
Citation
The zinc-binding region (ZBR) fragment of Emi2 can inhibit APC/C by targeting its association with the coactivator Cdc20 and UBE2C-mediated ubiquitylation
Shoji, S., Muto, Y., Ikeda, M., He, F., Tsuda, K., Ohsawa, N., Akasaka, R., Terada, T., Wakiyama, M., Shirouzu, M., Yokoyama, S.
FEBS Open Bio (2014), 4, 689-703, PubMed 25161877 , DOI 10.1016/j.fob.2014.06.010 ,
Related entities 1. a regulatory domain of meiosis inhibitor, entity 1, : 1 : 4 : 25 entities Detail
More details for 30 related entities
Interaction partners 1. a regulatory domain of meiosis inhibitor, entity 1, : 1 interactors Detail
More details for 1 interactors identified by 1 citations
Experiments performed 2 experiments Detail
nullKeywords solution structure, zinc-finger domain