BMRB: 11534

Solution structure of the GGQ domain of YaeJ protein from Escherichia coli

Authors

Nameki, N., Enomoto, M., Kogure, H., Tochio, N., Guntert, P.

Assembly

the GGQ domain of YaeJ protein

Entity

1. the GGQ domain of YaeJ protein (polymer), 121 monomers, 13451.21 Da Detail

MIVISRHVAI PDGELEITAI RAQGAGGQHV NKTSTAIHLR FDIRASSLPE YYKERLLAAS HHLISSDGVI VIKAQEYRSQ ELNREAALAR LVAMIKELTT EKKARRPTRS GPSSGENLYF Q

Formula weight

13451.21 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Refine. method

restrained energy minimization

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.8 %, Completeness (bb): 98.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	97.8 % (1363 of 1394)	97.1 % (705 of 726)	98.9 % (537 of 543)	96.8 % (121 of 125)
Backbone	98.5 % (707 of 718)	98.4 % (241 of 245)	99.2 % (353 of 356)	96.6 % (113 of 117)
Sidechain	97.6 % (771 of 790)	96.7 % (465 of 481)	99.0 % (298 of 301)	100.0 % (8 of 8)
Aromatic	97.2 % (70 of 72)	97.2 % (35 of 36)	97.2 % (35 of 36)
Methyl	100.0 % (152 of 152)	100.0 % (76 of 76)	100.0 % (76 of 76)

1. entity

MIVISRHVAI PDGELEITAI RAQGAGGQHV NKTSTAIHLR FDIRASSLPE YYKERLLAAS HHLISSDGVI VIKAQEYRSQ ELNREAALAR LVAMIKELTT EKKARRPTRS GPSSGENLYF Q

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	GGQ domain	[U-13C; U-15N]	protein	1 mM
2	TRIS	[U-2H]	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	DTT	[U-2H]		1 mM
5	NaN3	natural abundance		0.02 %
6	H2O	[U-2H]	solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	4.78 ppm	internal	indirect	0.2514495
1H	water	protons	4.78 ppm	internal	direct	1.0
15N	water	protons	4.78 ppm	internal	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	4.78 ppm	internal	indirect	0.2514495
1H	water	protons	4.78 ppm	internal	direct	1.0
15N	water	protons	4.78 ppm	internal	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.01 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	4.78 ppm	internal	indirect	0.2514495
1H	water	protons	4.78 ppm	internal	direct	1.0
15N	water	protons	4.78 ppm	internal	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.17 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	4.78 ppm	internal	indirect	0.2514495
1H	water	protons	4.78 ppm	internal	direct	1.0
15N	water	protons	4.78 ppm	internal	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2RTX, Strand ID: A Detail

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Release date

2013-12-22

Citation

Identification of residues required for stalled-ribosome rescue in the codon-independent release factor YaeJ
Kogure, H., Nameki, N.
Nucleic Acids Res. (2014), 42, 3152-3163, PubMed 24322300 , DOI 10.1093/nar/gkt1280 , Abstract

Related entities 1. the GGQ domain of YaeJ protein, : 1 : 160 entities Detail

Interaction partners 1. the GGQ domain of YaeJ protein, : 3 interactors Detail

Experiments performed 2 experiments Detail