BMRB: 11537

Solution structure of actinomycin

Authors

Umetsu, Y., Ohki, S.

Assembly

actinomycin

Entity

1. actinomycin (polymer, Thiol state: all disulfide bound), 36 monomers, 4071.567 Da Detail

GFGCPWNAYE CDRHCVSKGY TGGNCRGKIR QTCHCY

Formula weight

4071.567 Da

Entity Connection

disulfide 3 Detail

---

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS4:SG	1:CYS25:SG
2	disulfide	sing	1:CYS11:SG	1:CYS33:SG
3	disulfide	sing	1:CYS15:SG	1:CYS35:SG

Source organism

Actinomyces sp.

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.4 %, Completeness: 84.1 %, Completeness (bb): 90.9 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H
All	84.1 % (175 of 208)	84.1 % (175 of 208)
Backbone	90.9 % (70 of 77)	90.9 % (70 of 77)
Sidechain	80.2 % (105 of 131)	80.2 % (105 of 131)
Aromatic	66.7 % (18 of 27)	66.7 % (18 of 27)
Methyl	100.0 % (7 of 7)	100.0 % (7 of 7)

1. entity

GFGCPWNAYE CDRHCVSKGY TGGNCRGKIR QTCHCY

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 3.0

#	Name	Isotope labeling	Type	Concentration
1	entity	natural abundance	protein	2 mM
2	D2O		solvent	10 %
3	H2O		solvent	90 %

Hide sample codition

Protein Blocks Logo

Calculated from 20 models in PDB: 2RU0, Strand ID: A Detail

---

Release date

2014-10-19

Citation

Solution structure of actinomycin
Umetsu, Y., Ohki, S.
Not known

Related entities 1. actinomycin, : 1 : 39 entities Detail

Experiments performed 2 experiments Detail