BMRB: 11540

Solution structure of esf3

Authors

Umetsu, Y., Mori, M., Ohki, S.

Assembly

esf3

Entity

1. esf3 (polymer), 70 monomers, 7963.325 Da Detail

GPEKMEVKEI GRSSKIILPA CMHETCSGGF SLKNDCWCCL RLKTKQARCW KEKEFPNAKE LCFANCPPLE

Formula weight

7963.325 Da

Source organism

Arabidopsis thaliana

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.9 %, Completeness: 74.6 %, Completeness (bb): 75.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	74.6 % (624 of 836)	83.6 % (372 of 445)	57.5 % (184 of 320)	95.8 % (68 of 71)
Backbone	75.9 % (311 of 410)	92.8 % (129 of 139)	58.3 % (120 of 206)	95.4 % (62 of 65)
Sidechain	75.4 % (371 of 492)	79.4 % (243 of 306)	67.8 % (122 of 180)	100.0 % (6 of 6)
Aromatic	32.8 % (19 of 58)	58.6 % (17 of 29)	0.0 % (0 of 27)	100.0 % (2 of 2)
Methyl	96.2 % (50 of 52)	100.0 % (26 of 26)	92.3 % (24 of 26)

1. entity

GPEKMEVKEI GRSSKIILPA CMHETCSGGF SLKNDCWCCL RLKTKQARCW KEKEFPNAKE LCFANCPPLE

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.8

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-99% 13C; U-99% 15N]	protein	1 mM
2	sodium phosphate	natural abundance	buffer	50 mM
3	potassium chloride	natural abundance	salt	50 mM
4	H2O	natural abundance	solvent	90 %
5	D2O	natural abundance	solvent	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 5.8

#	Name	Isotope labeling	Type	Concentration
6	entity	[U-99% 13C; U-99% 15N]	protein	1 mM
7	sodium phosphate	natural abundance	buffer	50 mM
8	potassium chloride	natural abundance	salt	50 mM
9	D2O	natural abundance	solvent	100 %

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LACS Plot; CA

Referencing offset: 0.03 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.03 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.02 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2RU1, Strand ID: A Detail

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Release date

2014-04-21

Citation

Central cell-derived peptides regulate early embryo patterning in flowering plants
Costa, L.M., Marshall, E., Tesfaye, M., Silverstein, K.A.T., Mori, M., Umetsu, Y., Otterbach, S.L., Papareddy, R., Dickinson, H.G., Boutiller, K., VandenBosch, K.A., Ohki, S., Gutierrez-Marcos, J.F.
Science (2014), 344, 168-172, PubMed 24723605 , DOI 10.1126/science.1243005 , Abstract

Entries sharing articles Swiss-Prot: 14 entries Detail

Related entities 1. esf3, : 1 : 2 : 10 entities Detail

Experiments performed 9 experiments Detail