BMRB: 11541

Solution Structure of Protein-RNA Complex

Authors

Takahashi, M., Kuwasako, K., Unzai, S., Tsuda, K., Yoshikawa, S., He, F., Kobayashi, N., Guntert, P., Shirouzu, M., Ito, T., Tanaka, A., Yokoyama, S., Hagiwara, M., Kuroyanagi, H., Muto, Y.

Assembly

Protein-RNA Complex

Entity

1. Protein-RNA Complex, entity 1 (polymer, Thiol state: unknown), 103 monomers, 11410.64 Da Detail

GSTNAEPVVG SRDTMFTKIF VGGLPYHTSD KTLHEYFEQF GDIEEAVVIT DRNTQKSRGY GFVTMKDRAS AERACKDPNP IIDGRKANVN LAYLGAKPRT NVQ

2. RNA (polymer, Thiol state: not present), 6 monomers, 1971.146 Da Detail

GUGUGC

Total weight

13381.785 Da

Max. entity weight

11410.64 Da

Source organism

Caenorhabditis elegans

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.1 %, Completeness: 78.4 %, Completeness (bb): 71.8 % Detail

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Polymer type: polypeptide(L) polyribonucleotide

	Total	1H	13C	15N
All	78.4 % (993 of 1267)	83.7 % (553 of 661)	69.4 % (343 of 494)	86.6 % (97 of 112)
Backbone	71.8 % (484 of 674)	78.9 % (194 of 246)	60.3 % (199 of 330)	92.9 % (91 of 98)
Sidechain	86.9 % (597 of 687)	86.5 % (359 of 415)	89.9 % (232 of 258)	42.9 % (6 of 14)
Aromatic	72.0 % (85 of 118)	72.9 % (43 of 59)	77.8 % (42 of 54)	0.0 % (0 of 5)
Methyl	92.2 % (94 of 102)	90.2 % (46 of 51)	94.1 % (48 of 51)

1. entity 1

GSTNAEPVVG SRDTMFTKIF VGGLPYHTSD KTLHEYFEQF GDIEEAVVIT DRNTQKSRGY GFVTMKDRAS AERACKDPNP IIDGRKANVN LAYLGAKPRT NVQ

2. RNA

GUGUGC

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6 (±1), Details 20mM d-Tris-HCl(pH7.0),100mMNaCl,1mM d-DTT,0.02%NaN3

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]	protein	0.6 mM
2	RNA	natural abundance		0.72 mM
3	H2O		solvent	90 %
4	D2O		solvent	10 %
5	d-Tris-HCl		buffer	20 mM
6	NaCl	natural abundance	salt	100 mM
7	d-DTT			1 mM
8	NaN3	natural abundance		0.02 %

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LACS Plot; CA

Referencing offset: -0.02 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.02 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.02 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2RU3, Strand ID: A Detail

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Release date

2014-08-10

Citation

Solution Structure of Protein-RNA Complex
Kuwasako, K., Takahashi, M., Unzai, S., Tsuda, K., Yoshikawa, S., He, F., Kobayashi, N., Guntert, P., Shirouzu, M., Ito, T., Tanaka, A., Yokoyama, S., Hagiwara, M., Kuroyanagi, H., Muto, Y.
Not known

Related entities 1. Protein-RNA Complex, entity 1, : 1 : 1 : 1 : 117 entities Detail

Experiments performed 12 experiments Detail