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BMRB: 11549

2RU8

DnaT C-terminal domain

Authors

Abe, Y., Tani, J., Fujiyama, S., Urabe, M., Sato, K., Aramaki, T., Katayama, T., Ueda, T.

Assembly

DnaT

Entity

1. DnaT (polymer, Thiol state: not present), 98 monomers, 11220.48 Da Detail

MFAMYPDWQP DADFIRLAAL WGVALREPVT TEELASFIAY WQAEGKVFHH VQWQQKLARS LQIGRASNGG LPKRDVNTVS EPDSQIPPGF RGHHHHHH

Formula weight

11220.48 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.9 %, Completeness: 88.7 %, Completeness (bb): 91.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.7 % (1031 of 1163)	89.2 % (539 of 604)	87.3 % (397 of 455)	91.3 % (95 of 104)
Backbone	91.3 % (524 of 574)	92.9 % (182 of 196)	89.9 % (258 of 287)	92.3 % (84 of 91)
Sidechain	86.9 % (591 of 680)	87.5 % (357 of 408)	86.1 % (223 of 259)	84.6 % (11 of 13)
Aromatic	74.0 % (108 of 146)	74.0 % (54 of 73)	72.5 % (50 of 69)	100.0 % (4 of 4)
Methyl	94.7 % (89 of 94)	100.0 % (47 of 47)	89.4 % (42 of 47)

1. DnaT

MFAMYPDWQP DADFIRLAAL WGVALREPVT TEELASFIAY WQAEGKVFHH VQWQQKLARS LQIGRASNGG LPKRDVNTVS EPDSQIPPGF RGHHHHHH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	DnaT	[U-99% 13C; U-99% 15N]	protein	0.5 mM
2	HEPES	natural abundance		20 mM
3	H2O		solvent	90 %
4	D2O		solvent	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.24 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.24 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.39 ppm, Outliers: 7 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2RU8, Strand ID: A Detail

Release date

2014-10-05

Citation

Structure and a novel mechanism of the primosome protein DnaT: formation of DnaT-ssDNA complex and dissociation of ssDNA from PriB-ssDNA complex
Fujiyama, S., Abe, Y., Tani, J., Urabe, M., Sato, K., Aramaki, T., Katayama, T., Ueda, T.
J. Biol. Chem.

Related entities 1. DnaT, : 1 : 11 : 10 entities Detail

Interaction partners 1. DnaT, : 9 interactors Detail

More details for 9 interactors identified by 4 citations

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC