BMRB: 11581

NMR Structures and Localization of Potential Fusion Peptides and Pre-transmembrane Region of SARS-CoV: Implications in Membrane Fusion

Authors

Mahajan, M., Bhattacharjya, S.

Assembly

Pre transmembrane domain in DPC micelles

Entity

1. Pre transmembrane domain in DPC micelles (polymer, Thiol state: not present), 18 monomers, 2376.747 Da Detail

LGKYEQYIKW PWYVWLGF

Formula weight

2376.747 Da

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.4 %, Completeness: 44.4 %, Completeness (bb): 57.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C
All	44.4 % (107 of 241)	66.9 % (91 of 136)	15.2 % (16 of 105)
Backbone	57.3 % (51 of 89)	94.6 % (35 of 37)	30.8 % (16 of 52)
Sidechain	33.3 % (56 of 168)	56.6 % (56 of 99)	0.0 % (0 of 69)
Aromatic	25.4 % (17 of 67)	48.6 % (17 of 35)	0.0 % (0 of 32)
Methyl	31.3 % (5 of 16)	62.5 % (5 of 8)	0.0 % (0 of 8)

1. Pre transmembrane domain

LGKYEQYIKW PWYVWLGF

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Temperature 315 K, pH 4.7, Details Pre transmembrane domain in DPC micelles

#	Name	Isotope labeling	Type	Concentration
1	Pre transmembrane domain	natural abundance	peptide	0.5 mM
2	DPC	[U-99% 2H]		125 mM
3	H2O	natural abundance	solvent	90 %
4	D2O	[U-100% 2H]	solvent	10 %

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Calculated from 20 models in PDB: 2RUN, Strand ID: A Detail

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Release date

2016-07-12

Citation

NMR structures and localization of the potential fusion peptides and the pre-transmembrane region of SARS-CoV: Implications in membrane fusion
Mahajan, M., Bhattacharjya, S.
Biochim. Biophys. Acta (2015), 1848, 721-730, PubMed 25475644 , DOI 10.1016/j.bbamem.2014.11.025 , Abstract

Related entities 1. Pre transmembrane domain in DPC micelles, : 4 : 100 : 40 entities Detail

Experiments performed 3 experiments Detail