BMRB: 11582

NMR Structures and Localization of Potential Fusion Peptides and Pre-transmembrane Region of SARS-CoV: Implications in Membrane Fusion

Authors

Mahajan, M., Bhattacharjya, S.

Assembly

Internal fusion peptide in DPC micelles

Entity

1. Internal fusion peptide in DPC micelles (polymer, Thiol state: not present), 16 monomers, 1815.164 Da Detail

GAALQIPFAM QMAYRF

Formula weight

1815.164 Da

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 61.2 %, Completeness (bb): 59.5 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C
All	61.2 % (109 of 178)	93.1 % (94 of 101)	19.5 % (15 of 77)
Backbone	59.5 % (47 of 79)	100.0 % (32 of 32)	31.9 % (15 of 47)
Sidechain	54.4 % (62 of 114)	89.9 % (62 of 69)	0.0 % (0 of 45)
Aromatic	46.4 % (13 of 28)	92.9 % (13 of 14)	0.0 % (0 of 14)
Methyl	50.0 % (8 of 16)	100.0 % (8 of 8)	0.0 % (0 of 8)

1. Internal fusion peptide

GAALQIPFAM QMAYRF

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Temperature 315 K, pH 4.7, Details Internal fusion peptide in DPC micelles

#	Name	Isotope labeling	Type	Concentration
1	Internal fusion peptide	natural abundance	peptide	0.5 mM
2	DPC	[U-99% 2H]		125 mM
3	H2O	natural abundance	solvent	90 %
4	D2O	[U-100% 2H]	solvent	10 %

Hide sample codition

Protein Blocks Logo

Calculated from 20 models in PDB: 2RUO, Strand ID: A Detail

---

Release date

2016-07-12

Citation

NMR structures and localization of the potential fusion peptides and the pre-transmembrane region of SARS-CoV: Implications in membrane fusion
Mahajan, M., Bhattacharjya, S.
Biochim. Biophys. Acta (2015), 1848, 721-730, PubMed 25475644 , DOI 10.1016/j.bbamem.2014.11.025 , Abstract

Related entities 1. Internal fusion peptide in DPC micelles, : 102 : 2 entities Detail

Experiments performed 3 experiments Detail