Solution structure of Human Pin1 PPIase mutant C113A
GSHMEPARVR CSHLLVKHSQ SRRPSSWRQE KITRTKEEAL ELINGYIQKI KSGEEDFESL ASQFSDASSA KARGDLGAFS RGQMQKPFED ASFALRTGEM SGPVFTDSGI HIILRTE
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 97.8 % (1315 of 1345) | 98.3 % (695 of 707) | 97.5 % (504 of 517) | 95.9 % (116 of 121) |
Backbone | 96.5 % (670 of 694) | 97.1 % (232 of 239) | 96.5 % (330 of 342) | 95.6 % (108 of 113) |
Sidechain | 98.9 % (751 of 759) | 98.9 % (463 of 468) | 98.9 % (280 of 283) | 100.0 % (8 of 8) |
Aromatic | 99.0 % (95 of 96) | 100.0 % (48 of 48) | 97.9 % (46 of 47) | 100.0 % (1 of 1) |
Methyl | 99.0 % (99 of 100) | 98.0 % (49 of 50) | 100.0 % (50 of 50) |
1. entity
GSHMEPARVR CSHLLVKHSQ SRRPSSWRQE KITRTKEEAL ELINGYIQKI KSGEEDFESL ASQFSDASSA KARGDLGAFS RGQMQKPFED ASFALRTGEM SGPVFTDSGI HIILRTESolvent system 94% H2O/6% D2O, Pressure 1 atm, Temperature 299 K, pH 6.6
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | sodium sulfate | natural abundance | salt | 100 mM |
2 | sodium phosphate | natural abundance | buffer | 50 mM |
3 | EDTA | natural abundance | 5 mM | |
4 | D2O | [U-2H] | solvent | 6 % |
5 | H2O | natural abundance | solvent | 94 % |
6 | DTT | natural abundance | 1 mM | |
7 | NaN3 | natural abundance | 0.03 % |