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BMRB: 11599

2RVH

NMR structure of eIF1

Authors

NAGATA, T., OBAYASHI, E., ASANO, K.

Assembly

eIF1

Entity

1. eIF1 (polymer, Thiol state: all free), 108 monomers, 12312.00 Da Detail

MSIENLKSFD PFADTGDDET ATSNYIHIRI QQRNGRKTLT TVQGVPEEYD LKRILKVLKK DFACNGNIVK DPEMGEIIQL QGDQRAKVCE FMISQLGLQK KNIKIHGF

Formula weight

12312.0 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.1 %, Completeness: 92.7 %, Completeness (bb): 95.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.7 % (1206 of 1301)	91.0 % (624 of 686)	95.4 % (473 of 496)	91.6 % (109 of 119)
Backbone	95.8 % (615 of 642)	94.1 % (208 of 221)	97.5 % (308 of 316)	94.3 % (99 of 105)
Sidechain	90.8 % (689 of 759)	89.5 % (416 of 465)	93.9 % (263 of 280)	71.4 % (10 of 14)
Aromatic	78.4 % (58 of 74)	78.4 % (29 of 37)	78.4 % (29 of 37)
Methyl	98.3 % (114 of 116)	98.3 % (57 of 58)	98.3 % (57 of 58)

1. eIF1

MSIENLKSFD PFADTGDDET ATSNYIHIRI QQRNGRKTLT TVQGVPEEYD LKRILKVLKK DFACNGNIVK DPEMGEIIQL QGDQRAKVCE FMISQLGLQK KNIKIHGF

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	eIF1	[U-100% 13C; U-100% 15N]	protein	0.4 mM
2	sodium phosphate	natural abundance	buffer	20 mM
3	sodium chloride	natural abundance	salt	150 mM
4	DTT	natural abundance		1 mM
5	D2O	[U-2H]	solvent	5 %
6	H2O	natural abundance	solvent	95 %

LACS Plot; CA

Referencing offset: 0.03 ppm, Outliers: 2 Detail

LACS Plot; CB

Referencing offset: 0.03 ppm, Outliers: 2 Detail

LACS Plot; HA

Referencing offset: -0.06 ppm, Outliers: 4 Detail

LACS Plot; CO

Referencing offset: 0.1 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2RVH, Strand ID: A Detail

Release date

2016-10-19

Citation

NMR structure of eIF1
NAGATA, T., OBAYASHI, E.
Not known

Related entities 1. eIF1, : 1 : 9 : 1 : 73 entities Detail

Interaction partners 1. eIF1, : 27 interactors Detail

More details for 27 interactors identified by 7 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	eIF1	[U-100% 13C; U-100% 15N]	protein	0.4 mM
2	sodium phosphate	natural abundance	buffer	20 mM
3	sodium chloride	natural abundance	salt	150 mM
4	DTT	natural abundance		1 mM
5	D2O	[U-2H]	solvent	5 %
6	H2O	natural abundance	solvent	95 %

2 3D HNCO

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	eIF1	[U-100% 13C; U-100% 15N]	protein	0.4 mM
2	sodium phosphate	natural abundance	buffer	20 mM
3	sodium chloride	natural abundance	salt	150 mM
4	DTT	natural abundance		1 mM
5	D2O	[U-2H]	solvent	5 %
6	H2O	natural abundance	solvent	95 %

3 3D HN(CA)CO

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	eIF1	[U-100% 13C; U-100% 15N]	protein	0.4 mM
2	sodium phosphate	natural abundance	buffer	20 mM
3	sodium chloride	natural abundance	salt	150 mM
4	DTT	natural abundance		1 mM
5	D2O	[U-2H]	solvent	5 %
6	H2O	natural abundance	solvent	95 %

4 3D CBCA(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	eIF1	[U-100% 13C; U-100% 15N]	protein	0.4 mM
2	sodium phosphate	natural abundance	buffer	20 mM
3	sodium chloride	natural abundance	salt	150 mM
4	DTT	natural abundance		1 mM
5	D2O	[U-2H]	solvent	5 %
6	H2O	natural abundance	solvent	95 %

5 3D HNCACB

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	eIF1	[U-100% 13C; U-100% 15N]	protein	0.4 mM
2	sodium phosphate	natural abundance	buffer	20 mM
3	sodium chloride	natural abundance	salt	150 mM
4	DTT	natural abundance		1 mM
5	D2O	[U-2H]	solvent	5 %
6	H2O	natural abundance	solvent	95 %

6 3D C(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	eIF1	[U-100% 13C; U-100% 15N]	protein	0.4 mM
2	sodium phosphate	natural abundance	buffer	20 mM
3	sodium chloride	natural abundance	salt	150 mM
4	DTT	natural abundance		1 mM
5	D2O	[U-2H]	solvent	5 %
6	H2O	natural abundance	solvent	95 %

7 3D HBHA(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	eIF1	[U-100% 13C; U-100% 15N]	protein	0.4 mM
2	sodium phosphate	natural abundance	buffer	20 mM
3	sodium chloride	natural abundance	salt	150 mM
4	DTT	natural abundance		1 mM
5	D2O	[U-2H]	solvent	5 %
6	H2O	natural abundance	solvent	95 %

8 2D 1H-13C HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	eIF1	[U-100% 13C; U-100% 15N]	protein	0.4 mM
2	sodium phosphate	natural abundance	buffer	20 mM
3	sodium chloride	natural abundance	salt	150 mM
4	DTT	natural abundance		1 mM
5	D2O	[U-2H]	solvent	5 %
6	H2O	natural abundance	solvent	95 %

9 3D HCCH-TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	eIF1	[U-100% 13C; U-100% 15N]	protein	0.4 mM
2	sodium phosphate	natural abundance	buffer	20 mM
3	sodium chloride	natural abundance	salt	150 mM
4	DTT	natural abundance		1 mM
5	D2O	[U-2H]	solvent	5 %
6	H2O	natural abundance	solvent	95 %

10 3D HCCH-COSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	eIF1	[U-100% 13C; U-100% 15N]	protein	0.4 mM
2	sodium phosphate	natural abundance	buffer	20 mM
3	sodium chloride	natural abundance	salt	150 mM
4	DTT	natural abundance		1 mM
5	D2O	[U-2H]	solvent	5 %
6	H2O	natural abundance	solvent	95 %

11 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	eIF1	[U-100% 13C; U-100% 15N]	protein	0.4 mM
2	sodium phosphate	natural abundance	buffer	20 mM
3	sodium chloride	natural abundance	salt	150 mM
4	DTT	natural abundance		1 mM
5	D2O	[U-2H]	solvent	5 %
6	H2O	natural abundance	solvent	95 %

12 3D 1H-13C NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	eIF1	[U-100% 13C; U-100% 15N]	protein	0.4 mM
2	sodium phosphate	natural abundance	buffer	20 mM
3	sodium chloride	natural abundance	salt	150 mM
4	DTT	natural abundance		1 mM
5	D2O	[U-2H]	solvent	5 %
6	H2O	natural abundance	solvent	95 %

nullKeywords translation, translation initiation factor