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BMRB: 11601

2RVJ

pET22-hESRP1-RRM3

Authors

Yang, Y., Allemand, F., Guichou, J., Labesse, G.

Assembly

hESRP1-RRM3

Entity

1. hESRP1-RRM3 (polymer, Thiol state: all free), 104 monomers, 11817.54 Da Detail

MPPTNVRDCI RLRGLPYAAT IEDILDFLGE FATDIRTHGV HMVLNHQGRP SGDAFIQMKS ADRAFMAAQK CHKKNMKDRY VEVFQCSAEE MNFVLMGGTL NRLE

Formula weight

11817.54 Da

Source organism

Escherichia coli

Exptl. method

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.1 %, Completeness: 51.2 %, Completeness (bb): 96.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	51.2 % (620 of 1211)	35.8 % (226 of 632)	63.0 % (296 of 470)	89.9 % (98 of 109)
Backbone	96.1 % (592 of 616)	97.2 % (205 of 211)	95.1 % (290 of 305)	97.0 % (97 of 100)
Sidechain	17.2 % (119 of 692)	5.0 % (21 of 421)	37.0 % (97 of 262)	11.1 % (1 of 9)
Aromatic	0.0 % (0 of 92)	0.0 % (0 of 46)	0.0 % (0 of 46)
Methyl	13.5 % (14 of 104)	5.8 % (3 of 52)	21.2 % (11 of 52)

1. hESRP1-RRM3

MPPTNVRDCI RLRGLPYAAT IEDILDFLGE FATDIRTHGV HMVLNHQGRP SGDAFIQMKS ADRAFMAAQK CHKKNMKDRY VEVFQCSAEE MNFVLMGGTL NRLE

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 5.4

#	Name	Isotope labeling	Type	Concentration
1	hESRP1-RRM3	[U-99% 13C; U-99% 15N]	protein	0.6 mM
2	D2O	[U-2H]	solvent	10 %
3	H2O	natural abundance	solvent	90 %
4	Na2HPO4/KH2PO4	natural abundance	buffer	20 mM
5	NaCl	natural abundance	salt	150 mM

LACS Plot; CA

Referencing offset: -0.23 ppm, Outliers: 2 Detail

LACS Plot; CB

Referencing offset: -0.23 ppm, Outliers: 2 Detail

LACS Plot; HA

Referencing offset: -0.1 ppm, Outliers: 1 Detail

LACS Plot; CO

Referencing offset: -0.15 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 2RVJ, Strand ID: A Detail

Release date

2016-01-28

Citation

NMR structure of hESRP1-RRM3
Yang, Y., Allemand, F., Guichou, J., Labesse, G.
Not known

Related entities 1. hESRP1-RRM3, : 1 : 213 entities Detail

Interaction partners 1. hESRP1-RRM3, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DRX - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 5.4

#	Name	Isotope labeling	Type	Concentration
1	hESRP1-RRM3	[U-99% 13C; U-99% 15N]	protein	0.6 mM
2	D2O	[U-2H]	solvent	10 %
3	H2O	natural abundance	solvent	90 %
4	Na2HPO4/KH2PO4	natural abundance	buffer	20 mM
5	NaCl	natural abundance	salt	150 mM

2 3D 1H-13C NOESY aliphatic

Instrument

Bruker DRX - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 5.4

#	Name	Isotope labeling	Type	Concentration
1	hESRP1-RRM3	[U-99% 13C; U-99% 15N]	protein	0.6 mM
2	D2O	[U-2H]	solvent	10 %
3	H2O	natural abundance	solvent	90 %
4	Na2HPO4/KH2PO4	natural abundance	buffer	20 mM
5	NaCl	natural abundance	salt	150 mM

3 3D HNCA

Instrument

Bruker DRX - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 5.4

#	Name	Isotope labeling	Type	Concentration
1	hESRP1-RRM3	[U-99% 13C; U-99% 15N]	protein	0.6 mM
2	D2O	[U-2H]	solvent	10 %
3	H2O	natural abundance	solvent	90 %
4	Na2HPO4/KH2PO4	natural abundance	buffer	20 mM
5	NaCl	natural abundance	salt	150 mM

4 3D CBCA(CO)NH

Instrument

Bruker DRX - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 5.4

#	Name	Isotope labeling	Type	Concentration
1	hESRP1-RRM3	[U-99% 13C; U-99% 15N]	protein	0.6 mM
2	D2O	[U-2H]	solvent	10 %
3	H2O	natural abundance	solvent	90 %
4	Na2HPO4/KH2PO4	natural abundance	buffer	20 mM
5	NaCl	natural abundance	salt	150 mM

5 3D 1H-15N NOESY

Instrument

Bruker DRX - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 5.4

#	Name	Isotope labeling	Type	Concentration
1	hESRP1-RRM3	[U-99% 13C; U-99% 15N]	protein	0.6 mM
2	D2O	[U-2H]	solvent	10 %
3	H2O	natural abundance	solvent	90 %
4	Na2HPO4/KH2PO4	natural abundance	buffer	20 mM
5	NaCl	natural abundance	salt	150 mM

6 3D HNCO

Instrument

Bruker DRX - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 5.4

#	Name	Isotope labeling	Type	Concentration
1	hESRP1-RRM3	[U-99% 13C; U-99% 15N]	protein	0.6 mM
2	D2O	[U-2H]	solvent	10 %
3	H2O	natural abundance	solvent	90 %
4	Na2HPO4/KH2PO4	natural abundance	buffer	20 mM
5	NaCl	natural abundance	salt	150 mM

7 3D HBHA(CO)NH

Instrument

Bruker DRX - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 5.4

#	Name	Isotope labeling	Type	Concentration
1	hESRP1-RRM3	[U-99% 13C; U-99% 15N]	protein	0.6 mM
2	D2O	[U-2H]	solvent	10 %
3	H2O	natural abundance	solvent	90 %
4	Na2HPO4/KH2PO4	natural abundance	buffer	20 mM
5	NaCl	natural abundance	salt	150 mM

nullKeywords hESRP1-RRM3