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BMRB: 11609

2RVQ

Solution structure of the isolated histone H2A-H2B heterodimer

Authors

Moriwaki, Y., Yamane, T., Ohtomo, H., Ikeguchi, M., Kurita, J., Sato, M., Nagadoi, A., Shimojo, H., Nishimura, Y.

Assembly

histone H2A-H2B heterodimer

Entity

1. histone H2A-H2B heterodimer, entity 1 (polymer, Thiol state: all free), 133 monomers, 14346.52 Da Detail

GPGMSGRGKQ GGKARAKAKT RSSRAGLQFP VGRVHRLLRK GNYSERVGAG APVYLAAVLE YLTAEILELA GNAARDNKKT RIIPRHLQLA IRNDEELNKL LGRVTIAQGG VLPNIQAVLL PKKTESHHKA KGK

2. histone H2A-H2B heterodimer, entity 2 (polymer, Thiol state: all free), 129 monomers, 14115.20 Da Detail

GPGMPEPAKS APAPKKGSKK AVTKAQKKDG KKRKRSRKES YSIYVYKVLK QVHPDTGISS KAMGIMNSFV NDIFERIAGE ASRLAHYNKR STITSREIQT AVRLLLPGEL AKHAVSEGTK AVTKYTSAK

Total weight

28461.719 Da

Max. entity weight

14346.52 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

CS-Rosetta-AbinitioRelax, CS-Rosetta-FloppyTail

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 58.4 %, Completeness: 28.6 %, Completeness (bb): 46.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	28.6 % (869 of 3042)	19.0 % (306 of 1607)	36.7 % (429 of 1169)	50.4 % (134 of 266)
Backbone	46.1 % (712 of 1546)	33.6 % (180 of 536)	52.2 % (397 of 761)	54.2 % (135 of 249)
Sidechain	16.2 % (281 of 1733)	12.0 % (128 of 1071)	23.7 % (153 of 645)	0.0 % (0 of 17)
Aromatic	4.9 % (6 of 122)	9.8 % (6 of 61)	0.0 % (0 of 61)
Methyl	16.9 % (49 of 290)	13.8 % (20 of 145)	20.0 % (29 of 145)

1. entity 1

GPGMSGRGKQ GGKARAKAKT RSSRAGLQFP VGRVHRLLRK GNYSERVGAG APVYLAAVLE YLTAEILELA GNAARDNKKT RIIPRHLQLA IRNDEELNKL LGRVTIAQGG VLPNIQAVLL PKKTESHHKA KGK

2. entity 2

GPGMPEPAKS APAPKKGSKK AVTKAQKKDG KKRKRSRKES YSIYVYKVLK QVHPDTGISS KAMGIMNSFV NDIFERIAGE ASRLAHYNKR STITSREIQT AVRLLLPGEL AKHAVSEGTK AVTKYTSAK

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-13C; U-15N; U-2H]	protein	0.1 ~ 0.3 mM
2	entity_2	[U-13C; U-15N; U-2H]	protein	0.1 ~ 0.3 mM
3	MES	natural abundance	buffer	25 mM
4	KCl	natural abundance	salt	400 mM
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-99% 2H]	solvent	10 %

Chem. Shift Complete2

Sequence coverage: 56.5 %, Completeness: 29.1 %, Completeness (bb): 46.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	29.1 % (1773 of 6084)	18.8 % (605 of 3214)	38.1 % (890 of 2338)	52.3 % (278 of 532)
Backbone	46.8 % (1448 of 3092)	32.9 % (353 of 1072)	54.0 % (822 of 1522)	54.8 % (273 of 498)
Sidechain	16.9 % (586 of 3466)	11.9 % (254 of 2142)	25.3 % (326 of 1290)	17.6 % (6 of 34)
Aromatic	4.9 % (12 of 244)	9.8 % (12 of 122)	0.0 % (0 of 122)
Methyl	14.7 % (85 of 580)	11.0 % (32 of 290)	18.3 % (53 of 290)

1. entity 1

GPGMSGRGKQ GGKARAKAKT RSSRAGLQFP VGRVHRLLRK GNYSERVGAG APVYLAAVLE YLTAEILELA GNAARDNKKT RIIPRHLQLA IRNDEELNKL LGRVTIAQGG VLPNIQAVLL PKKTESHHKA KGK

2. entity 2

GPGMPEPAKS APAPKKGSKK AVTKAQKKDG KKRKRSRKES YSIYVYKVLK QVHPDTGISS KAMGIMNSFV NDIFERIAGE ASRLAHYNKR STITSREIQT AVRLLLPGEL AKHAVSEGTK AVTKYTSAK

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-13C; U-15N; U-2H]	protein	0.1 ~ 0.3 mM
2	entity_2	[U-13C; U-15N; U-2H]	protein	0.1 ~ 0.3 mM
3	MES	natural abundance	buffer	25 mM
4	KCl	natural abundance	salt	400 mM
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-99% 2H]	solvent	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.42 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.42 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2RVQ, Strand ID: C, D Detail

Release date

2016-05-22

Citation

Solution structure of the isolated histone H2A-H2B heterodimer
Moriwaki, Y., Yamane, T., Ohtomo, H., Ikeguchi, M., Kurita, J., Sato, M., Nagadoi, A., Shimojo, H., Nishimura, Y.
Sci. Rep. (2016), 6, 24999-24999, PubMed 27181506 , DOI 10.1038/srep24999 , Abstract

Related entities 1. histone H2A-H2B heterodimer, entity 1, : 19 : 186 entities Detail

Related entities 2. histone H2A-H2B heterodimer, entity 2, : 3 : 149 entities Detail

Interaction partners 1. histone H2A-H2B heterodimer, entity 1, : 76 : 1 interactors Detail

More details for 77 interactors identified by 16 citations

Interaction partners 2. histone H2A-H2B heterodimer, entity 2, : 12 : 1 interactors Detail

More details for 13 interactors identified by 7 citations

Experiments performed 7 experiments Detail

1 TROSY-HN(CO)CACB