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BMRB: 15023

2JML

1H, and 15N Chemical Shift Assignments for the N-terminal domain of Myxococcus xantus CarA protein.

Authors

Jimenez, M., Padmanabhan, S., Gonzalez, C., Perez-Marin, M.C., Elias-Arnanz, M., Murillo, F.J., Rico, M.

Assembly

CarA(Nter)

Entity

1. CarA(Nter) (polymer, Thiol state: not present), 81 monomers, 9454.653 Da Detail

GSHMTLRIRT IARMTGIREA TLRAWERRYG FPRPLRSEGN NYRVYSREEV EAVRRVARLI QEEGLSVSEA IAQVKTEPPR E

Formula weight

9454.653 Da

Source organism

Myxococcus xanthus

Exptl. method

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.8 %, Completeness: 92.7 %, Completeness (bb): 93.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	92.7 % (545 of 588)	92.9 % (470 of 506)	91.5 % (75 of 82)
Backbone	93.8 % (225 of 240)	95.1 % (155 of 163)	90.9 % (70 of 77)
Sidechain	92.0 % (320 of 348)	91.8 % (315 of 343)	100.0 % (5 of 5)
Aromatic	100.0 % (26 of 26)	100.0 % (25 of 25)	100.0 % (1 of 1)
Methyl	100.0 % (44 of 44)	100.0 % (44 of 44)

1. CarA(Nter)

GSHMTLRIRT IARMTGIREA TLRAWERRYG FPRPLRSEGN NYRVYSREEV EAVRRVARLI QEEGLSVSEA IAQVKTEPPR E

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 100 mM NaCl 50 mM phosphate buffer DSS

#	Name	Isotope labeling	Type	Concentration
1	CarA(Nter)	natural abundance		0.5 ~ 1.0 mM
2	H2O			90 %
3	D2O			10 %
4	DSS			0.1 ~ 0.5 mM
5	sodium chloride			100 mM
6	sodium phosphate			50 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 100 mM NaCl 50 mM phosphate buffer DSS

#	Name	Isotope labeling	Type	Concentration
7	CarA(Nter)	natural abundance		0.5 ~ 1.0 mM
8	D2O			100 %
9	DSS			0.1 ~ 0.5 mM
10	sodium chloride			100 mM
11	sodium phosphate			50 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 100 mM NaCl 50 mM phosphate buffer DSS

#	Name	Isotope labeling	Type	Concentration
12	CarA(Nter)	[U-100% 15N]		0.5 ~ 1.0 mM
13	H2O			90 %
14	D2O			10 %
15	DSS			0.1 ~ 0.5 mM
16	sodium chloride			100 mM
17	sodium phosphate			50 mM

Protein Blocks Logo

Calculated from 20 models in PDB: 2JML, Strand ID: A Detail

Release date

2007-02-07

Citation

Structural basis for operator and antirepressor recognition by Myxococcus xanthus CarA repressor
Navarro-Aviles, G., Jimenez, M., Perez-Marin, M.C., Gonzalez, C., Rico, M., Murillo, F.J., Elias-Arnanz, M., Padmanabhan, S.
Mol. Microbiol. (2007), 63, 980-994, PubMed 17233828 , DOI 10.1111/j.1365-2958.2006.05567.x , Abstract

Related entities 1. CarA(Nter), : 1 : 1 : 20 entities Detail

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AV - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 100 mM NaCl 50 mM phosphate buffer DSS

#	Name	Isotope labeling	Type	Concentration
12	CarA(Nter)	[U-100% 15N]		0.5 ~ 1.0 mM
13	H2O			90 %
14	D2O			10 %
15	DSS			0.1 ~ 0.5 mM
16	sodium chloride			100 mM
17	sodium phosphate			50 mM

2 3D 1H-15N NOESY

Instrument

Bruker AV - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 100 mM NaCl 50 mM phosphate buffer DSS

#	Name	Isotope labeling	Type	Concentration
12	CarA(Nter)	[U-100% 15N]		0.5 ~ 1.0 mM
13	H2O			90 %
14	D2O			10 %
15	DSS			0.1 ~ 0.5 mM
16	sodium chloride			100 mM
17	sodium phosphate			50 mM

3 3D 1H-15N TOCSY

Instrument

Bruker AV - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 100 mM NaCl 50 mM phosphate buffer DSS

#	Name	Isotope labeling	Type	Concentration
12	CarA(Nter)	[U-100% 15N]		0.5 ~ 1.0 mM
13	H2O			90 %
14	D2O			10 %
15	DSS			0.1 ~ 0.5 mM
16	sodium chloride			100 mM
17	sodium phosphate			50 mM

4 2D 1H-1H NOESY

Instrument

Bruker AV - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 100 mM NaCl 50 mM phosphate buffer DSS

#	Name	Isotope labeling	Type	Concentration
12	CarA(Nter)	[U-100% 15N]		0.5 ~ 1.0 mM
13	H2O			90 %
14	D2O			10 %
15	DSS			0.1 ~ 0.5 mM
16	sodium chloride			100 mM
17	sodium phosphate			50 mM

5 2D 1H-1H TOCSY

Instrument

Bruker AV - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 100 mM NaCl 50 mM phosphate buffer DSS

#	Name	Isotope labeling	Type	Concentration
12	CarA(Nter)	[U-100% 15N]		0.5 ~ 1.0 mM
13	H2O			90 %
14	D2O			10 %
15	DSS			0.1 ~ 0.5 mM
16	sodium chloride			100 mM
17	sodium phosphate			50 mM

6 2D 1H-1H NOESY

Instrument

Bruker AV - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 100 mM NaCl 50 mM phosphate buffer DSS

#	Name	Isotope labeling	Type	Concentration
1	CarA(Nter)	natural abundance		0.5 ~ 1.0 mM
2	H2O			90 %
3	D2O			10 %
4	DSS			0.1 ~ 0.5 mM
5	sodium chloride			100 mM
6	sodium phosphate			50 mM

7 2D 1H-1H TOCSY

Instrument

Bruker AV - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 100 mM NaCl 50 mM phosphate buffer DSS

#	Name	Isotope labeling	Type	Concentration
1	CarA(Nter)	natural abundance		0.5 ~ 1.0 mM
2	H2O			90 %
3	D2O			10 %
4	DSS			0.1 ~ 0.5 mM
5	sodium chloride			100 mM
6	sodium phosphate			50 mM

8 2D 1H-1H COSY

Instrument

Bruker AV - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 100 mM NaCl 50 mM phosphate buffer DSS

#	Name	Isotope labeling	Type	Concentration
1	CarA(Nter)	natural abundance		0.5 ~ 1.0 mM
2	H2O			90 %
3	D2O			10 %
4	DSS			0.1 ~ 0.5 mM
5	sodium chloride			100 mM
6	sodium phosphate			50 mM

9 2D 1H-1H NOESY

Instrument

Bruker AV - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 100 mM NaCl 50 mM phosphate buffer DSS

#	Name	Isotope labeling	Type	Concentration
7	CarA(Nter)	natural abundance		0.5 ~ 1.0 mM
8	D2O			100 %
9	DSS			0.1 ~ 0.5 mM
10	sodium chloride			100 mM
11	sodium phosphate			50 mM

10 2D 1H-1H TOCSY

Instrument

Bruker AV - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 100 mM NaCl 50 mM phosphate buffer DSS

#	Name	Isotope labeling	Type	Concentration
7	CarA(Nter)	natural abundance		0.5 ~ 1.0 mM
8	D2O			100 %
9	DSS			0.1 ~ 0.5 mM
10	sodium chloride			100 mM
11	sodium phosphate			50 mM

11 2D 1H-1H COSY

Instrument

Bruker AV - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 100 mM NaCl 50 mM phosphate buffer DSS

#	Name	Isotope labeling	Type	Concentration
7	CarA(Nter)	natural abundance		0.5 ~ 1.0 mM
8	D2O			100 %
9	DSS			0.1 ~ 0.5 mM
10	sodium chloride			100 mM
11	sodium phosphate			50 mM

nullKeywords DNA binding, light response, Myxococcus xanthus, NMR, repressor-antirepressor