BMRBj - BMREntryMaster

Found 1 Document (1.011 seconds)

BMRB: 15038

2OFN

1H, 13C, and 15N resonance assignments of FK506-binding domain of Plasmodium falciparum FKBP35

Authors

Kang, C., Ye, H., Yoon, H., Yoon, H.

Assembly

pfFKBP35FKBD

Entity

1. pfFKBP35FKBD (polymer, Thiol state: all free), 135 monomers, 15336.09 Da Detail

MTTEQEFEKV ELTADGGVIK TILKKGDEGE ENIPKKGNEV TVHYVGKLES TGKVFDSSFD RNVPFKFHLE QGEVIKGWDI CVSSMRKNEK CLVRIESMYG YGDEGCGESI PGNSVLLFEI ELLSFRELEH HHHHH

Formula weight

15336.09 Da

Source organism

Plasmodium falciparum

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.6 %, Completeness: 89.1 %, Completeness (bb): 94.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.1 % (1411 of 1583)	92.2 % (761 of 825)	84.0 % (519 of 618)	93.6 % (131 of 140)
Backbone	94.2 % (757 of 804)	94.3 % (265 of 281)	94.1 % (368 of 391)	93.9 % (124 of 132)
Sidechain	85.1 % (766 of 900)	91.2 % (496 of 544)	75.6 % (263 of 348)	87.5 % (7 of 8)
Aromatic	37.7 % (52 of 138)	75.4 % (52 of 69)	0.0 % (0 of 68)	0.0 % (0 of 1)
Methyl	96.9 % (126 of 130)	96.9 % (63 of 65)	96.9 % (63 of 65)

1. pfFKBP35FKBD

MTTEQEFEKV ELTADGGVIK TILKKGDEGE ENIPKKGNEV TVHYVGKLES TGKVFDSSFD RNVPFKFHLE QGEVIKGWDI CVSSMRKNEK CLVRIESMYG YGDEGCGESI PGNSVLLFEI ELLSFRELEH HHHHH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 0.5 mM in a buffer containing 20 mM Na-PO4, 50 mM NaCl, 1 mM DTT, 0.1mM EDTA, 10% D2O

#	Name	Isotope labeling	Concentration
1	pfFKBP35FKBD	[U-13C; U-15N]	0.5 mM
2	Na-PO4		20 mM
3	NaCl		50 mM
4	DTT		1 mM
5	EDTA		0.1 mM
6	D2O		10 %
7	H2O		90 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.3 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.3 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.44 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2OFN, Strand ID: A Detail

Release date

2007-06-26

Citation

1H, 13C, and 15N resonance assignments of FK506-binding domain of Plasmodium falciparum FKBP35
Kang, C., Ye, H., Yoon, H., Yoon, H.
Biomol. NMR Assign. (2007), 1, 27-28, PubMed 19636818 , DOI 10.1007/s12104-007-9005-4 , Abstract

Related entities 1. pfFKBP35FKBD, : 1 : 2 : 242 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 0.5 mM in a buffer containing 20 mM Na-PO4, 50 mM NaCl, 1 mM DTT, 0.1mM EDTA, 10% D2O

#	Name	Isotope labeling	Concentration
1	pfFKBP35FKBD	[U-13C; U-15N]	0.5 mM
2	Na-PO4		20 mM
3	NaCl		50 mM
4	DTT		1 mM
5	EDTA		0.1 mM
6	D2O		10 %
7	H2O		90 %

2 3D HNCA

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	pfFKBP35FKBD	[U-13C; U-15N]	0.5 mM
2	Na-PO4		20 mM
3	NaCl		50 mM
4	DTT		1 mM
5	EDTA		0.1 mM
6	D2O		10 %
7	H2O		90 %

3 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	pfFKBP35FKBD	[U-13C; U-15N]	0.5 mM
2	Na-PO4		20 mM
3	NaCl		50 mM
4	DTT		1 mM
5	EDTA		0.1 mM
6	D2O		10 %
7	H2O		90 %

4 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	pfFKBP35FKBD	[U-13C; U-15N]	0.5 mM
2	Na-PO4		20 mM
3	NaCl		50 mM
4	DTT		1 mM
5	EDTA		0.1 mM
6	D2O		10 %
7	H2O		90 %

5 3D HN(CO)CA

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	pfFKBP35FKBD	[U-13C; U-15N]	0.5 mM
2	Na-PO4		20 mM
3	NaCl		50 mM
4	DTT		1 mM
5	EDTA		0.1 mM
6	D2O		10 %
7	H2O		90 %

6 3D HCCH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	pfFKBP35FKBD	[U-13C; U-15N]	0.5 mM
2	Na-PO4		20 mM
3	NaCl		50 mM
4	DTT		1 mM
5	EDTA		0.1 mM
6	D2O		10 %
7	H2O		90 %

7 3D 1H-15N NOESY

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	pfFKBP35FKBD	[U-13C; U-15N]	0.5 mM
2	Na-PO4		20 mM
3	NaCl		50 mM
4	DTT		1 mM
5	EDTA		0.1 mM
6	D2O		10 %
7	H2O		90 %

8 3D HCC(CO)NH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	pfFKBP35FKBD	[U-13C; U-15N]	0.5 mM
2	Na-PO4		20 mM
3	NaCl		50 mM
4	DTT		1 mM
5	EDTA		0.1 mM
6	D2O		10 %
7	H2O		90 %

9 3D (H)CC(CO)NH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	pfFKBP35FKBD	[U-13C; U-15N]	0.5 mM
2	Na-PO4		20 mM
3	NaCl		50 mM
4	DTT		1 mM
5	EDTA		0.1 mM
6	D2O		10 %
7	H2O		90 %

10 (H)CCH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	pfFKBP35FKBD	[U-13C; U-15N]	0.5 mM
2	Na-PO4		20 mM
3	NaCl		50 mM
4	DTT		1 mM
5	EDTA		0.1 mM
6	D2O		10 %
7	H2O		90 %

nullKeywords NMR

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Found 1 Document (1.011 seconds)

BMRB: 15038

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. pfFKBP35FKBD, : 1 : 2 : 242 entities Detail

Experiments performed 10 experiments Detail

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