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BMRB: 15055

2JMP

Structure for the N-terminus of chromosomal replication initiation protein dnaA from M. genitalium

Authors

Lowery, T.J., Pelton, J.G., Wemmer, D.E.

Assembly

N-term of dnaA

Entity

1. N-term of dnaA (polymer, Thiol state: not present), 100 monomers, 11855.20 Da Detail

MEQFNAFKSL LKKHYEKTIG FHDKYIKDIN RFVFKNNVLL ILLENEFARN SLNDNSEIIH LAESLYEGIK SVNFVNEQDF FFNLAKLEEN SRDTLYQNSG

Formula weight

11855.2 Da

Source organism

Mycoplasma genitalium

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.0 %, Completeness: 81.6 %, Completeness (bb): 95.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	81.6 % (1025 of 1256)	78.4 % (514 of 656)	84.9 % (411 of 484)	86.2 % (100 of 116)
Backbone	95.8 % (575 of 600)	96.6 % (196 of 203)	94.3 % (280 of 297)	99.0 % (99 of 100)
Sidechain	72.4 % (545 of 753)	70.2 % (318 of 453)	79.6 % (226 of 284)	6.3 % (1 of 16)
Aromatic	36.8 % (53 of 144)	40.3 % (29 of 72)	33.3 % (24 of 72)
Methyl	95.2 % (99 of 104)	94.2 % (49 of 52)	96.2 % (50 of 52)

1. N dnaA

MEQFNAFKSL LKKHYEKTIG FHDKYIKDIN RFVFKNNVLL ILLENEFARN SLNDNSEIIH LAESLYEGIK SVNFVNEQDF FFNLAKLEEN SRDTLYQNSG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±2) K, pH 6.8 (±0.2), Details uniformly 15N-labeled

#	Name	Isotope labeling	Concentration
1	N_dnaA	[U-100% 15N]	0.75 (±0.2) mM
2	sodium phosphate		50 (±10.0) mM
3	sodium chloride		100 (±10.0) mM
4	EDTA		2 (±1.0) mM
5	sodium azide		0.05 (±0.01) %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±2) K, pH 6.8 (±0.2), Details uniformly 13C/15N-labeled

#	Name	Isotope labeling	Concentration
6	N_dnaA	[U-98% 13C; U-98% 15N]	0.75 (±0.2) mM
7	sodium phosphate		50 (±10.0) mM
8	sodium chloride		100 (±10.0) mM
9	EDTA		2 (±1.0) mM
10	sodium azide		0.05 (±0.01) %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±2) K, pH 6.8 (±0.2), Details unlabeled

#	Name	Isotope labeling	Concentration
11	N_dnaA	natural abundance	0.75 (±0.2) mM
12	sodium phosphate		50 (±10.0) mM
13	sodium chloride		100 (±10.0) mM
14	EDTA		2 (±1.0) mM
15	sodium azide		0.05 (±0.01) %

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±2) K, pH 6.8 (±0.2), Details unlabeled

#	Name	Isotope labeling	Concentration
16	N_dnaA	[U-98% 13C; U-98% 15N]	0.75 (±0.2) mM
17	sodium phosphate		50 (±10.0) mM
18	sodium chloride		100 (±10.0) mM
19	EDTA		2 (±1.0) mM
20	sodium azide		0.05 (±0.01) %

Sample #5

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±2) K, pH 6.8 (±0.2), Details 10% 13C labeled

#	Name	Isotope labeling	Concentration
21	N_dnaA	[10% 13C]	0.75 (±0.2) mM
22	sodium phosphate	natural abundance	50 (±10.0) mM
23	sodium chloride	natural abundance	100 (±10.0) mM
24	EDTA	natural abundance	2 (±1.0) mM
25	sodium azide	natural abundance	0.05 (±0.01) %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.16 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2JMP, Strand ID: A Detail

Release date

2007-10-15

Citation

NMR structure of the N-terminal domain of the replication initiator protein DnaA
Lowery, T.J., Pelton, J.G., Chandonia, J., Kim, R., Yokota, H., Wemmer, D.E.
J. Struct. Funct. Genomics (2007), 8, 11-17, PubMed 17680349 , DOI 10.1007/s10969-007-9022-7 , Abstract

Related entities 1. N-term of dnaA, : 1 : 1 : 1 entities Detail

Experiments performed 23 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±2) K, pH 6.8 (±0.2), Details uniformly 15N-labeled

#	Name	Isotope labeling	Concentration
1	N_dnaA	[U-100% 15N]	0.75 (±0.2) mM
2	sodium phosphate		50 (±10.0) mM
3	sodium chloride		100 (±10.0) mM
4	EDTA		2 (±1.0) mM
5	sodium azide		0.05 (±0.01) %

2 3D HNCA

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±2) K, pH 6.8 (±0.2), Details uniformly 13C/15N-labeled

#	Name	Isotope labeling	Concentration
6	N_dnaA	[U-98% 13C; U-98% 15N]	0.75 (±0.2) mM
7	sodium phosphate		50 (±10.0) mM
8	sodium chloride		100 (±10.0) mM
9	EDTA		2 (±1.0) mM
10	sodium azide		0.05 (±0.01) %

3 3D CBCA(CO)NH

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±2) K, pH 6.8 (±0.2), Details uniformly 13C/15N-labeled

#	Name	Isotope labeling	Concentration
6	N_dnaA	[U-98% 13C; U-98% 15N]	0.75 (±0.2) mM
7	sodium phosphate		50 (±10.0) mM
8	sodium chloride		100 (±10.0) mM
9	EDTA		2 (±1.0) mM
10	sodium azide		0.05 (±0.01) %

4 3D HNCACB

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±2) K, pH 6.8 (±0.2), Details uniformly 13C/15N-labeled

#	Name	Isotope labeling	Concentration
6	N_dnaA	[U-98% 13C; U-98% 15N]	0.75 (±0.2) mM
7	sodium phosphate		50 (±10.0) mM
8	sodium chloride		100 (±10.0) mM
9	EDTA		2 (±1.0) mM
10	sodium azide		0.05 (±0.01) %

5 3D HNCO

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±2) K, pH 6.8 (±0.2), Details uniformly 13C/15N-labeled

#	Name	Isotope labeling	Concentration
6	N_dnaA	[U-98% 13C; U-98% 15N]	0.75 (±0.2) mM
7	sodium phosphate		50 (±10.0) mM
8	sodium chloride		100 (±10.0) mM
9	EDTA		2 (±1.0) mM
10	sodium azide		0.05 (±0.01) %

6 3D C(CO)NH

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±2) K, pH 6.8 (±0.2), Details uniformly 13C/15N-labeled

#	Name	Isotope labeling	Concentration
6	N_dnaA	[U-98% 13C; U-98% 15N]	0.75 (±0.2) mM
7	sodium phosphate		50 (±10.0) mM
8	sodium chloride		100 (±10.0) mM
9	EDTA		2 (±1.0) mM
10	sodium azide		0.05 (±0.01) %

7 3d HN(CA)CO

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±2) K, pH 6.8 (±0.2), Details uniformly 13C/15N-labeled

#	Name	Isotope labeling	Concentration
6	N_dnaA	[U-98% 13C; U-98% 15N]	0.75 (±0.2) mM
7	sodium phosphate		50 (±10.0) mM
8	sodium chloride		100 (±10.0) mM
9	EDTA		2 (±1.0) mM
10	sodium azide		0.05 (±0.01) %

8 3D HNHA

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±2) K, pH 6.8 (±0.2), Details uniformly 15N-labeled

#	Name	Isotope labeling	Concentration
1	N_dnaA	[U-100% 15N]	0.75 (±0.2) mM
2	sodium phosphate		50 (±10.0) mM
3	sodium chloride		100 (±10.0) mM
4	EDTA		2 (±1.0) mM
5	sodium azide		0.05 (±0.01) %

9 3D HN(CA)CO

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±2) K, pH 6.8 (±0.2), Details uniformly 13C/15N-labeled

#	Name	Isotope labeling	Concentration
6	N_dnaA	[U-98% 13C; U-98% 15N]	0.75 (±0.2) mM
7	sodium phosphate		50 (±10.0) mM
8	sodium chloride		100 (±10.0) mM
9	EDTA		2 (±1.0) mM
10	sodium azide		0.05 (±0.01) %

10 3D H(CCO)NH

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±2) K, pH 6.8 (±0.2), Details uniformly 13C/15N-labeled

#	Name	Isotope labeling	Concentration
6	N_dnaA	[U-98% 13C; U-98% 15N]	0.75 (±0.2) mM
7	sodium phosphate		50 (±10.0) mM
8	sodium chloride		100 (±10.0) mM
9	EDTA		2 (±1.0) mM
10	sodium azide		0.05 (±0.01) %

11 3D 1H-15N TOCSY

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±2) K, pH 6.8 (±0.2), Details uniformly 15N-labeled

#	Name	Isotope labeling	Concentration
1	N_dnaA	[U-100% 15N]	0.75 (±0.2) mM
2	sodium phosphate		50 (±10.0) mM
3	sodium chloride		100 (±10.0) mM
4	EDTA		2 (±1.0) mM
5	sodium azide		0.05 (±0.01) %

12 3D HCCH-TOCSY

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±2) K, pH 6.8 (±0.2), Details uniformly 13C/15N-labeled

#	Name	Isotope labeling	Concentration
6	N_dnaA	[U-98% 13C; U-98% 15N]	0.75 (±0.2) mM
7	sodium phosphate		50 (±10.0) mM
8	sodium chloride		100 (±10.0) mM
9	EDTA		2 (±1.0) mM
10	sodium azide		0.05 (±0.01) %

13 3D H(CCO)NH

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±2) K, pH 6.8 (±0.2), Details uniformly 13C/15N-labeled

#	Name	Isotope labeling	Concentration
6	N_dnaA	[U-98% 13C; U-98% 15N]	0.75 (±0.2) mM
7	sodium phosphate		50 (±10.0) mM
8	sodium chloride		100 (±10.0) mM
9	EDTA		2 (±1.0) mM
10	sodium azide		0.05 (±0.01) %

14 3D HBHA(CO)NH

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±2) K, pH 6.8 (±0.2), Details uniformly 13C/15N-labeled

#	Name	Isotope labeling	Concentration
6	N_dnaA	[U-98% 13C; U-98% 15N]	0.75 (±0.2) mM
7	sodium phosphate		50 (±10.0) mM
8	sodium chloride		100 (±10.0) mM
9	EDTA		2 (±1.0) mM
10	sodium azide		0.05 (±0.01) %

15 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±2) K, pH 6.8 (±0.2), Details uniformly 15N-labeled

#	Name	Isotope labeling	Concentration
1	N_dnaA	[U-100% 15N]	0.75 (±0.2) mM
2	sodium phosphate		50 (±10.0) mM
3	sodium chloride		100 (±10.0) mM
4	EDTA		2 (±1.0) mM
5	sodium azide		0.05 (±0.01) %

16 3D HNHA

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±2) K, pH 6.8 (±0.2), Details uniformly 15N-labeled

#	Name	Isotope labeling	Concentration
1	N_dnaA	[U-100% 15N]	0.75 (±0.2) mM
2	sodium phosphate		50 (±10.0) mM
3	sodium chloride		100 (±10.0) mM
4	EDTA		2 (±1.0) mM
5	sodium azide		0.05 (±0.01) %

17 3D 1H-13C NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±2) K, pH 6.8 (±0.2), Details unlabeled

#	Name	Isotope labeling	Concentration
16	N_dnaA	[U-98% 13C; U-98% 15N]	0.75 (±0.2) mM
17	sodium phosphate		50 (±10.0) mM
18	sodium chloride		100 (±10.0) mM
19	EDTA		2 (±1.0) mM
20	sodium azide		0.05 (±0.01) %

18 3D 1H-13C NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±2) K, pH 6.8 (±0.2), Details unlabeled

#	Name	Isotope labeling	Concentration
16	N_dnaA	[U-98% 13C; U-98% 15N]	0.75 (±0.2) mM
17	sodium phosphate		50 (±10.0) mM
18	sodium chloride		100 (±10.0) mM
19	EDTA		2 (±1.0) mM
20	sodium azide		0.05 (±0.01) %

19 4D HCCH NOESY

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±2) K, pH 6.8 (±0.2), Details unlabeled

#	Name	Isotope labeling	Concentration
16	N_dnaA	[U-98% 13C; U-98% 15N]	0.75 (±0.2) mM
17	sodium phosphate		50 (±10.0) mM
18	sodium chloride		100 (±10.0) mM
19	EDTA		2 (±1.0) mM
20	sodium azide		0.05 (±0.01) %

20 2D 1H-13C HSQC

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±2) K, pH 6.8 (±0.2), Details 10% 13C labeled

#	Name	Isotope labeling	Concentration
21	N_dnaA	[10% 13C]	0.75 (±0.2) mM
22	sodium phosphate	natural abundance	50 (±10.0) mM
23	sodium chloride	natural abundance	100 (±10.0) mM
24	EDTA	natural abundance	2 (±1.0) mM
25	sodium azide	natural abundance	0.05 (±0.01) %

21 2D 1H-1H NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±2) K, pH 6.8 (±0.2), Details unlabeled

#	Name	Isotope labeling	Concentration
11	N_dnaA	natural abundance	0.75 (±0.2) mM
12	sodium phosphate		50 (±10.0) mM
13	sodium chloride		100 (±10.0) mM
14	EDTA		2 (±1.0) mM
15	sodium azide		0.05 (±0.01) %

22 2D DQF-COSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±2) K, pH 6.8 (±0.2), Details unlabeled

#	Name	Isotope labeling	Concentration
11	N_dnaA	natural abundance	0.75 (±0.2) mM
12	sodium phosphate		50 (±10.0) mM
13	sodium chloride		100 (±10.0) mM
14	EDTA		2 (±1.0) mM
15	sodium azide		0.05 (±0.01) %

23 2D 1H-1H TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±2) K, pH 6.8 (±0.2), Details unlabeled

#	Name	Isotope labeling	Concentration
11	N_dnaA	natural abundance	0.75 (±0.2) mM
12	sodium phosphate		50 (±10.0) mM
13	sodium chloride		100 (±10.0) mM
14	EDTA		2 (±1.0) mM
15	sodium azide		0.05 (±0.01) %

NMR combined restraints 7 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints, RDC restraints	combined_15055_2jmp.nef
Input source #2: Coordindates	2jmp.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

---------------10--------20--------30--------40--------50--------60--------70--------80--------90---
MGGGGGGMEQFNAFKSLLKKHYEKTIGFHDKYIKDINRFVFKNNVLLILLENEFARNSLNDNSEIIHLAESLYEGIKSVNFVNEQDFFFNLAKLEENSRD
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
MGGGGGGMEQFNAFKSLLKKHYEKTIGFHDKYIKDINRFVFKNNVLLILLENEFARNSLNDNSEIIHLAESLYEGIKSVNFVNEQDFFFNLAKLEENSRD
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100

----100
TLYQNSG
|||||||
TLYQNSG
-------

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	107	0	0	100.0

Content subtype: combined_15055_2jmp.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	1002		92.5 (chain: A, length: 107)
1	Distance restraints	CNS/XPLOR_distance_constraints_7	Warning	991 (1)	noe	89.7 (chain: A, length: 107)
2	Distance restraints	CNS/XPLOR_distance_constraints_3	OK	50 (1)	hbond	39.3 (chain: A, length: 107)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_4	OK	46 (46)	.	66.4 (chain: A, length: 107)
1	RDC restraints	CNS/XPLOR_dipolar_coupling_5	OK	35 (35)	.	32.7 (chain: A, length: 107)
2	RDC restraints	CNS/XPLOR_dipolar_coupling_6	OK	53 (53)	.	49.5 (chain: A, length: 107)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 107, Sequence coverage: 92.5%
- Total number of assignments
  - ¹H chemical shifts: 500
  - ¹³C chemical shifts: 403
  - ¹⁵N chemical shifts: 99
- Completeness of assignments
  - Entity_assemble_ID: A
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	681	500	73.4
¹³C chemical shifts	501	403	80.4
¹⁵N chemical shifts	126	99	78.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	223	200	89.7
¹³C chemical shifts	214	182	85.0
¹⁵N chemical shifts	107	99	92.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	458	300	65.5
¹³C chemical shifts	287	221	77.0
¹⁵N chemical shifts	19	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	54	51	94.4
¹³C chemical shifts	54	51	94.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	72	20	27.8
¹³C chemical shifts	72	18	25.0

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_7
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 107, Sequence coverage: 89.7%
- Total number of restraints: 991
- Weight of restraints: 1.0 (991)
- Potential type of restraints: square-well-parabolic (991)
- Range of distance target values: 2.15, 4.75 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map
- Saveframe: CNS/XPLOR_distance_constraints_3
  - Status: OK
  - Experiment type: hbond
  - Sequence coverage of experimental data
    - Entity_assembly_ID: A, Chain length: 107, Sequence coverage: 39.3%
  - Total number of restraints: 50
  - Weight of restraints: 1.0 (50)
  - Potential type of restraints: square-well-parabolic (50)
  - Range of distance target values: 1.55, 2.55 (Å)
  - Histogram of distance target values
  - Distance restraints per residue
    - Chain_ID: A
  - Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_4
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 107, Sequence coverage: 66.4%
- Total number of restraints: 46
- Total number of restraints per polymer type: 46
- Weight of restraints: 1.0 (46)
- Potential type of restraints: square-well-parabolic (46)
- Dihedral angle restraints per residue
  - Chain_ID: A

RDC restraints

Saveframe: CNS/XPLOR_dipolar_coupling_5
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 107, Sequence coverage: 32.7%
- Total number of restraints: 35
- Potential type of restraints: undefined (35)
- Range of observed RDC values: 100.0, -100.0 (Hz)
- RDC restraints per residue
- Saveframe: CNS/XPLOR_dipolar_coupling_6
  - Status: OK
  - Experiment type: .
  - Sequence coverage of experimental data
    - Entity_assembly_ID: A, Chain length: 107, Sequence coverage: 49.5%
  - Total number of restraints: 53
  - Potential type of restraints: undefined (53)
  - Range of observed RDC values: 100.0, -100.0 (Hz)
  - RDC restraints per residue

Keywords bacteria, DNA replication, solution structure, domain, N-terminal, protein

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Found 1 Document (0.865 seconds)

BMRB: 15055

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. N-term of dnaA, : 1 : 1 : 1 entities Detail

Experiments performed 23 experiments Detail

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