BMRB: 15059

1H, 13C, and 15N Chemical Shift Assignments for the muscular LIM protein MLP/CRP3.

Authors

Thomas, S., Claudia, M.

Assembly

MLP

Entity

1. MLP (polymer, Thiol state: free and other bound), 194 monomers, 20942.66 Da Detail

MANWGGGAKC GACEKTVYHA EEIQCNGRSF HKTCFHCMAC RKALDSTTVA AHESEIYCKV CYGRRYGPKG IGYGQGAGCL STDTGEHLGL QFQQSPKPAR SVTTSNPSKF TAKFGESEKC PRCGKSVYAA EKVMGGGKPW HKTCFRCAIC GKSLESTNVT DKDGELYCKV CYAKNFGPTG IGFGGLTQQV EKKE

2. ZN (non-polymer), 65.409 × 4 Da

Total weight

21204.297 Da

Max. entity weight

20942.66 Da

Entity Connection

na 16 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	2:ZN1:ZN	1:CYS10:SG
2	na	sing	2:ZN1:ZN	1:CYS13:SG
3	na	sing	2:ZN1:ZN	1:CYS34:SG
4	na	sing	2:ZN1:ZN	1:HIS31:ND1
5	na	sing	2:ZN1:ZN	1:CYS37:SG
6	na	sing	2:ZN1:ZN	1:CYS40:SG
7	na	sing	2:ZN1:ZN	1:CYS58:SG
8	na	sing	2:ZN1:ZN	1:CYS61:SG
9	na	sing	2:ZN1:ZN	1:CYS120:SG
10	na	sing	2:ZN1:ZN	1:CYS123:SG
11	na	sing	2:ZN1:ZN	1:CYS144:SG
12	na	sing	2:ZN1:ZN	1:HIS141:ND1
13	na	sing	2:ZN1:ZN	1:CYS147:SG
14	na	sing	2:ZN1:ZN	1:CYS150:SG
15	na	sing	2:ZN1:ZN	1:CYS168:SG
16	na	sing	2:ZN1:ZN	1:CYS171:SG

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 85.6 %, Completeness: 66.8 %, Completeness (bb): 71.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	66.8 % (1441 of 2158)	66.5 % (748 of 1125)	63.2 % (526 of 832)	83.1 % (167 of 201)
Backbone	71.4 % (821 of 1150)	79.1 % (322 of 407)	61.5 % (342 of 556)	84.0 % (157 of 187)
Sidechain	64.7 % (761 of 1176)	59.3 % (426 of 718)	73.2 % (325 of 444)	71.4 % (10 of 14)
Aromatic	51.0 % (98 of 192)	51.0 % (49 of 96)	50.0 % (47 of 94)	100.0 % (2 of 2)
Methyl	83.8 % (119 of 142)	83.1 % (59 of 71)	84.5 % (60 of 71)

1. MLP

MANWGGGAKC GACEKTVYHA EEIQCNGRSF HKTCFHCMAC RKALDSTTVA AHESEIYCKV CYGRRYGPKG IGYGQGAGCL STDTGEHLGL QFQQSPKPAR SVTTSNPSKF TAKFGESEKC PRCGKSVYAA EKVMGGGKPW HKTCFRCAIC GKSLESTNVT DKDGELYCKV CYAKNFGPTG IGFGGLTQQV EKKE

Show sample condition

Sample

Pressure 1 atm, Temperature 295 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	MLP	[U-100% 13C; U-100% 15N]		1.0 mM
2	ZINC (II) ION	none		1.0 mM
3	KHPO4	none		20 mM
4	KCl	none		150 mM
5	DTT	none		2 mM

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LACS Plot; CA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	4.79 ppm	external	indirect	0.25145
1H	water	protons	4.79 ppm	internal	direct	1.0
15N	water	protons	4.79 ppm	external	indirect	0.10132

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LACS Plot; CB

Referencing offset: -0.04 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	4.79 ppm	external	indirect	0.25145
1H	water	protons	4.79 ppm	internal	direct	1.0
15N	water	protons	4.79 ppm	external	indirect	0.10132

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LACS Plot; HA

Referencing offset: 0.08 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	4.79 ppm	external	indirect	0.25145
1H	water	protons	4.79 ppm	internal	direct	1.0
15N	water	protons	4.79 ppm	external	indirect	0.10132

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Protein Blocks Logo

Calculated from 19 models in PDB: 2O10, Strand ID: A Detail

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Release date

2007-06-25

Citation

1H, 13C, and 15N assignment of the muscular LIM protein MLP/CRP3
Schallus, T., Edlich, C., Stier, G., Muhle-Goll, C.
Biomol. NMR Assign. (2007), 1, 41-43, PubMed 19636821 , DOI 10.1007/s12104-007-9010-7 , Abstract

Related entities 1. MLP, : 2 : 154 entities Detail

Interaction partners 1. MLP, : 9 interactors Detail

Experiments performed 6 experiments Detail