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BMRB: 15086

2JN6

Solution NMR structure of Hypothetical Protein Cgl2762 from Corynebacterium Glutamicum: Northeast Structural Genomics Consortium Target CgR3

Authors

Singarapu, K., sukumaran, D., Parish, D., Chen, C.X., Kellie, C., Rong, X., G V T, S., Montelione, G.T., Szyperski, T.

Assembly

hypothetical protein Cgl2762

Entity

1. hypothetical protein Cgl2762 (polymer, Thiol state: not present), 97 monomers, 11025.09 Da Detail

MPTKTYSEEF KRDAVALYEN SDGASLQQIA NDLGINRVTL KNWIIKYGSN HNVQGTTPSA AVSEAEQIRQ LKKENALQRA RTRHPAESCL EHHHHHH

Formula weight

11025.09 Da

Source organism

Corynebacterium glutamicum

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.0 %, Completeness: 97.4 %, Completeness (bb): 98.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	97.4 % (1091 of 1120)	98.3 % (575 of 585)	96.3 % (411 of 427)	97.2 % (105 of 108)
Backbone	98.4 % (567 of 576)	99.0 % (193 of 195)	97.9 % (281 of 287)	98.9 % (93 of 94)
Sidechain	96.7 % (616 of 637)	97.9 % (382 of 390)	95.3 % (222 of 233)	85.7 % (12 of 14)
Aromatic	89.7 % (70 of 78)	100.0 % (39 of 39)	78.9 % (30 of 38)	100.0 % (1 of 1)
Methyl	100.0 % (96 of 96)	100.0 % (48 of 48)	100.0 % (48 of 48)

1. hypothetical protein Cgl2762

MPTKTYSEEF KRDAVALYEN SDGASLQQIA NDLGINRVTL KNWIIKYGSN HNVQGTTPSA AVSEAEQIRQ LKKENALQRA RTRHPAESCL EHHHHHH

Show sample condition

Sample

Pressure 1 atm, Temperature 298 (±1) K, pH 4.4 (±.1)

#	Name	Isotope labeling	Type	Concentration
1	hypothetical protein Cgl2762	[U-100% 13C; U-100% 15N]		1.2 (±0.2) mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.82 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.82 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -1.23 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2JN6, Strand ID: A Detail

Release date

2007-02-22

Citation

NMR structure of protein Cgl2762 from Corynebacterium glutamicum implicated in DNA transposition reveals a helix-turn-helix motif attached to a flexibly disordered leucine zipper
Singarapu, K., Xiao, R., Sukumaran, D.K., Acton, T., Montelione, G.T., Szyperski, T.
Proteins (2008), 70, 1650-1654, PubMed 18175328 , DOI 10.1002/prot.21840 , Abstract

Related entities 1. hypothetical protein Cgl2762, : 1 : 9 entities Detail

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC