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BMRB: 15098

2CKA

SOLUTION STRUCTURES OF THE BRK DOMAINS OF THE HUMAN CHROMO HELICASE DOMAIN 7 AND 8, REVEALS STRUCTURAL SIMILARITY WITH GYF DOMAIN SUGGESTING A ROLE IN PROTEIN INTERACTION

Authors

AB, E., De Jong, R.N., Diercks, T., Xiaoyun, J., Daniels, M., Kaptein, R., Folkers, G.E.

Assembly

CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 8

Entity

1. CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 8 (polymer, Thiol state: all free), 95 monomers, 11038.47 Da Detail

MGSSHHHHHH SSHMLVPRGS LKKLVELEVE CMEEPNHLDV DLETRIPVIN KVDGTLLVGE DAPRRAELEM WLQGHPEFAV DPRFLAYMED RRKQK

Formula weight

11038.47 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

CANDID IN CYANA

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 61.1 %, Completeness: 55.8 %, Completeness (bb): 59.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	55.8 % (625 of 1120)	54.1 % (318 of 588)	57.1 % (250 of 438)	60.6 % (57 of 94)
Backbone	59.0 % (329 of 558)	58.7 % (111 of 189)	58.9 % (165 of 280)	59.6 % (53 of 89)
Sidechain	53.7 % (350 of 652)	51.9 % (207 of 399)	56.0 % (139 of 248)	80.0 % (4 of 5)
Aromatic	51.3 % (39 of 76)	52.6 % (20 of 38)	48.6 % (18 of 37)	100.0 % (1 of 1)
Methyl	70.8 % (68 of 96)	70.8 % (34 of 48)	70.8 % (34 of 48)

1. CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 8

MGSSHHHHHH SSHMLVPRGS LKKLVELEVE CMEEPNHLDV DLETRIPVIN KVDGTLLVGE DAPRRAELEM WLQGHPEFAV DPRFLAYMED RRKQK

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1.0 atm, Temperature 298.0 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 8	[U-13C; U-15N]	0.8 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	150 mM
4	sodium azide	natural abundance	0.02 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2CKA, Strand ID: A Detail

Release date

2012-08-02

Citation

Solution Structures of the Brk Domains of the Human Chromo Helicase Domain 7 and 8, Reveals Structural Similarity with Gyf Domain Suggesting a Role in Protein Interaction
AB, E., De Jong, R.N., Diercks, T., Xiaoyun, J., Daniels, M., Kaptein, R., Folkers, G.E.

Related entities 1. CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 8, : 1 : 12 entities Detail

Interaction partners 1. CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 8, : 32 interactors Detail

More details for 32 interactors identified by 13 citations

Experiments performed 13 experiments Detail

1 HNCO