BMRB: 15103

Structure of NOWA cysteine rich domain 6

Authors

Meier, S., Jensen, P.R., Adamczyk, P., Bachinger, H.P., Holstein, T.W., Engel, J., Ozbek, S., Grzesiek, S.

Assembly

NW domain 6

Entity

1. NW domain 6 (polymer, Thiol state: all disulfide bound), 27 monomers, 2776.065 Da Detail

GSSSCPQFPS CSPSCAPQCS QQCCQQP

Formula weight

2776.065 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS5:SG	1:CYS19:SG
2	disulfide	sing	1:CYS11:SG	1:CYS24:SG
3	disulfide	sing	1:CYS15:SG	1:CYS23:SG

Source organism

Hydra vulgaris

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 77.8 %, Completeness: 23.6 %, Completeness (bb): 58.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	23.6 % (42 of 178)	14.0 % (21 of 150)	75.0 % (21 of 28)
Backbone	58.3 % (42 of 72)	42.0 % (21 of 50)	95.5 % (21 of 22)
Sidechain	0.0 % (0 of 106)	0.0 % (0 of 100)	0.0 % (0 of 6)
Aromatic	0.0 % (0 of 5)	0.0 % (0 of 5)
Methyl	0.0 % (0 of 1)	0.0 % (0 of 1)

1. Nematocyst outer wall antigen

GSSSCPQFPS CSPSCAPQCS QQCCQQP

Show sample condition

Sample

Solvent system 95% H2O, 5% D2O, Pressure 1 atm, Temperature 288 K, pH 5.5, Details 0.5 mM peptide, 15N, 5 mM phosphate buffer, 95% H2O, 5% D2O

#	Name	Isotope labeling	Type	Concentration
1	Nematocyst outer wall antigen	[U-100% 15N]		0.5 mM
2	D2O	[U-2H]		5 %
3	potassium phosphate	natural abundance		5 mM

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Protein Blocks Logo

Calculated from 10 models in PDB: 2NX6, Strand ID: A Detail

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Release date

2007-10-16

Citation

Sequence-structure and structure-function analysis in cysteine-rich domains forming the ultrastable nematocyst wall
Meier, S., Jensen, P.R., Adamczyk, P., Bachinger, H.P., Holstein, T.W., Engel, J., Oezbek, S., Grzesiek, S.
J. Mol. Biol. (2007), 368, 718-728, PubMed 17362991 , DOI 10.1016/j.jmb.2007.02.026 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. NW domain 6, : 1 entities Detail

Experiments performed 4 experiments Detail