BMRB: 15104

Structure of NOWA cysteine rich domain 8

Authors

Meier, S., Jensen, P.R., Adamczyk, P., Bachinger, H.P., Holstein, T.W., Engel, J., Ozbek, S., Grzesiek, S.

Assembly

NW domain 8

Entity

1. NW domain 8 (polymer, Thiol state: all disulfide bound), 30 monomers, 2900.296 Da Detail

GSAQNPCSLQ QPGCSSACAP ACRLSCCSLG

Formula weight

2900.296 Da

Entity Connection

disulfide 3 Detail

---

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS7:SG	1:CYS22:SG
2	disulfide	sing	1:CYS14:SG	1:CYS27:SG
3	disulfide	sing	1:CYS18:SG	1:CYS26:SG

Source organism

Hydra vulgaris

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 86.7 %, Completeness: 30.4 %, Completeness (bb): 60.9 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	15N
All	30.4 % (55 of 181)	19.3 % (29 of 150)	83.9 % (26 of 31)
Backbone	60.9 % (53 of 87)	45.0 % (27 of 60)	96.3 % (26 of 27)
Sidechain	2.1 % (2 of 94)	2.2 % (2 of 90)	0.0 % (0 of 4)
Methyl	0.0 % (0 of 10)	0.0 % (0 of 10)

1. Nematocyst outer wall antigen

GSAQNPCSLQ QPGCSSACAP ACRLSCCSLG

Show sample condition

Sample

Solvent system 95 % H2O, 5% D2O, Pressure 1 atm, Temperature 288 K, pH 5.5, Details 0.5 mM peptide, pH 5.5, 95% H2O, 5% D2O

#	Name	Isotope labeling	Type	Concentration
1	Nematocyst outer wall antigen NW8	[U-100% 15N]		0.5 mM
2	D2O	[U-2H]		5 %
3	potassium phosphate	natural abundance		5 mM

Hide sample codition

Protein Blocks Logo

Calculated from 10 models in PDB: 2NX7, Strand ID: A Detail

---

Release date

2007-10-16

Citation

Sequence-structure and structure-function analysis in cysteine-rich domains forming the ultrastable nematocyst wall
Meier, S., Jensen, P.R., Adamczyk, P., Bachinger, H.P., Holstein, T.W., Engel, J., Oezbek, S., Grzesiek, S.
J. Mol. Biol. (2007), 368, 718-728, PubMed 17362991 , DOI 10.1016/j.jmb.2007.02.026 , Abstract

Related entities 1. NW domain 8, : 1 : 1 entities Detail

Experiments performed 4 experiments Detail