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NMR structure of p1 from the infectious bursal disease virus (IBDV) in dodecylphosphocholin (DPC).
Authors
Galloux, M., Libersou, S., Morellet, N., Bouaziz, S., Ouldali, M., Da Costa, B., Lepault, J., Delmas, B.
Assembly
p1
Entity
1. p1 (polymer, Thiol state: not present), 46 monomers, 4876.609 Da Detail

FGFKDIIRAI RRIAVPVVST LFPPAAPLAH AIGEGVDYLL GDEAQA


Formula weight
4876.609 Da
Source organism
Infectious bursal disease virus
Exptl. method
solution NMR
Refine. method
distance geometry, simulated annealing, energy minimization
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 97.8 %, Completeness: 93.6 %, Completeness (bb): 97.8 % Detail

Polymer type: polypeptide(L)

Total1H
All93.6 % (248 of 265)93.6 % (248 of 265)
Backbone97.8 % (90 of 92)97.8 % (90 of 92)
Sidechain91.3 % (158 of 173)91.3 % (158 of 173)
Aromatic61.9 % (13 of 21)61.9 % (13 of 21)
Methyl100.0 % (35 of 35)100.0 % (35 of 35)

1. IBDV p1 peptide

FGFKDIIRAI RRIAVPVVST LFPPAAPLAH AIGEGVDYLL GDEAQA

Sample

Solvent system 90%H20, 10%D2O, Pressure 1 atm, Temperature 333 K, pH 3.5, Details 1mM pep46; 40mM DPC; 90%H2O, 10%D2O


#NameIsotope labelingTypeConcentration
1pep46natural abundance1 mM
2DPCnatural abundance40 mM

Protein Blocks Logo
Calculated from 13 models in PDB: 2IMU, Strand ID: A Detail


Release date
2007-10-16
Citation
Infectious bursal disease virus, a non-enveloped virus, possesses a capsid-associated peptide that deforms and perforates biological membranes
Galloux, M., Libersou, S., Morellet, N., Bouaziz, S., Da Costa, B., Ouldali, M., Lepault, J., Delmas, B.
J. Biol. Chem. (2007), 282, 20774-20784, PubMed 17488723 , DOI 10.1074/jbc.M701048200 ,
Entries sharing articles Swiss-Prot: 1 entries Detail
  Swiss-Prot: P61825 released on 2004-06-07
    Title POLS_IBDV Entity Structural polyprotein
Related entities 1. p1, : 1 : 6 : 1 : 1 : 65 entities Detail
Experiments performed 2 experiments Detail
nullKeywords BIRNAVIRUS, DPC, IBDV, p4, PORE FORMATION, VP2