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BMRB: 15109

2JNG

Solution structure of the CUL7-CPH domain from Homo Sapiens; Northeast Structural Genomics Consortium target HT1.

Authors

Lemak, A., Kaustov, L., Lukin, J., Duan, S., Arrowsmith, C.H.

Assembly

CPH domain

Entity

1. CPH domain (polymer, Thiol state: not present), 105 monomers, 12002.12 Da Detail

GSHMRSEFAS GNTYALYVRD TLQPGMRVRM LDDYEEISAG DEGEFRQSNN GVPPVQVFWE STGRTYWVHW HMLEILGFEE DIEDMVEADE YQGAVASRVL GRALP

Formula weight

12002.12 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 91.4 %, Completeness (bb): 96.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	91.4 % (1095 of 1198)	93.7 % (580 of 619)	87.4 % (409 of 468)	95.5 % (106 of 111)
Backbone	96.0 % (597 of 622)	97.7 % (211 of 216)	95.1 % (290 of 305)	95.0 % (96 of 101)
Sidechain	87.9 % (590 of 671)	91.6 % (369 of 403)	81.8 % (211 of 258)	100.0 % (10 of 10)
Aromatic	67.2 % (86 of 128)	79.7 % (51 of 64)	52.5 % (32 of 61)	100.0 % (3 of 3)
Methyl	88.8 % (87 of 98)	93.9 % (46 of 49)	83.7 % (41 of 49)

1. CPH domain

GSHMRSEFAS GNTYALYVRD TLQPGMRVRM LDDYEEISAG DEGEFRQSNN GVPPVQVFWE STGRTYWVHW HMLEILGFEE DIEDMVEADE YQGAVASRVL GRALP

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	CPH domain	[U-15N; U-13C]	0.7 mM
2	TRIS	natural abundance	25 mM
3	sodium chloride	natural abundance	250 mM
4	H2O	natural abundance	55 M
5	PMSF	natural abundance	0.5 mM
6	TCEP	natural abundance	0.5 mM
7	Benzamidine	natural abundance	1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.16 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2JNG, Strand ID: A Detail

Release date

2007-05-20

Citation

The conserved CPH domains of Cul7 and PARC are protein-protein interaction modules that bind the tetramerization domain of p53
Kaustov, L., Lukin, J., Lemak, A., Duan, S., Ho, M., Doherty, R., Penn, L.Z., Arrowsmith, C.H.
J. Biol. Chem. (2007), 282, 11300-11307, PubMed 17298945 , DOI 10.1074/jbc.M611297200 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: Q14999 released on 1997-11-01
Title CUL7_HUMAN Entity Cullin-7

Related entities 1. CPH domain, : 1 : 2 : 9 entities Detail

Interaction partners 1. CPH domain, : 14 interactors Detail

More details for 14 interactors identified by 13 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC