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BMRB: 15152

2OT2

Hydrogenase isoenzymes formation protein HypC

Authors

Wang, L., Jin, C.

Assembly

HypC

Entity

1. HypC (polymer, Thiol state: all free), 90 monomers, 9731.897 Da Detail

MCIGVPGQIR TIDGNQAKVD VCGIQRDVDL TLVGSCDENG QPRVGQWVLV HVGFAMSVIN EAEARDTLDA LQNMFDVEPD VGALLYGEEK

Formula weight

9731.897 Da

Source organism

Escherichia coli

Exptl. method

Refine. method

distance geometry, torsion angle dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.9 %, Completeness: 95.6 %, Completeness (bb): 97.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	95.6 % (945 of 988)	95.7 % (486 of 508)	95.0 % (363 of 382)	98.0 % (96 of 98)
Backbone	97.6 % (521 of 534)	97.3 % (182 of 187)	97.7 % (254 of 260)	97.7 % (85 of 87)
Sidechain	94.2 % (503 of 534)	94.7 % (304 of 321)	93.1 % (188 of 202)	100.0 % (11 of 11)
Aromatic	59.1 % (26 of 44)	59.1 % (13 of 22)	57.1 % (12 of 21)	100.0 % (1 of 1)
Methyl	100.0 % (114 of 114)	100.0 % (57 of 57)	100.0 % (57 of 57)

1. HypC

MCIGVPGQIR TIDGNQAKVD VCGIQRDVDL TLVGSCDENG QPRVGQWVLV HVGFAMSVIN EAEARDTLDA LQNMFDVEPD VGALLYGEEK

Show sample condition

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	HypC	[U-95% 15N]		1 mM
2	potassium phosphate			30 mM
3	KCl			30 mM
4	dithiothreitol			25 uM
5	sodium azide			0.02 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
6	HypC	[U-95% 13C; U-95% 15N]		1 mM
7	potassium phosphate			30 mM
8	KCl			30 mM
9	dithiothreitol			25 uM
10	sodium azide			0.02 %

LACS Plot; CA

Referencing offset: -0.22 ppm, Outliers: 2 Detail

LACS Plot; CB

Referencing offset: -0.22 ppm, Outliers: 2 Detail

LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Referencing offset: -5.02 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2OT2, Strand ID: A Detail

Release date

2007-10-23

Citation

Solution structure of Escherichia coli HypC
Wang, L., Xia, B., Jin, C.
Biochem. Biophys. Res. Commun. (2007), 361, 665-669, PubMed 17669368 , DOI 10.1016/j.bbrc.2007.07.094 , Abstract

Related entities 1. HypC, : 1 : 2 : 15 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	HypC	[U-95% 15N]		1 mM
2	potassium phosphate			30 mM
3	KCl			30 mM
4	dithiothreitol			25 uM
5	sodium azide			0.02 %

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
6	HypC	[U-95% 13C; U-95% 15N]		1 mM
7	potassium phosphate			30 mM
8	KCl			30 mM
9	dithiothreitol			25 uM
10	sodium azide			0.02 %

3 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
6	HypC	[U-95% 13C; U-95% 15N]		1 mM
7	potassium phosphate			30 mM
8	KCl			30 mM
9	dithiothreitol			25 uM
10	sodium azide			0.02 %

4 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
6	HypC	[U-95% 13C; U-95% 15N]		1 mM
7	potassium phosphate			30 mM
8	KCl			30 mM
9	dithiothreitol			25 uM
10	sodium azide			0.02 %

5 3D HBHA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
6	HypC	[U-95% 13C; U-95% 15N]		1 mM
7	potassium phosphate			30 mM
8	KCl			30 mM
9	dithiothreitol			25 uM
10	sodium azide			0.02 %

6 3D HCCH-COSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
6	HypC	[U-95% 13C; U-95% 15N]		1 mM
7	potassium phosphate			30 mM
8	KCl			30 mM
9	dithiothreitol			25 uM
10	sodium azide			0.02 %

7 3D C(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
6	HypC	[U-95% 13C; U-95% 15N]		1 mM
7	potassium phosphate			30 mM
8	KCl			30 mM
9	dithiothreitol			25 uM
10	sodium azide			0.02 %

8 3D 1H-15N TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	HypC	[U-95% 15N]		1 mM
2	potassium phosphate			30 mM
3	KCl			30 mM
4	dithiothreitol			25 uM
5	sodium azide			0.02 %

9 3D 1H-15N NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	HypC	[U-95% 15N]		1 mM
2	potassium phosphate			30 mM
3	KCl			30 mM
4	dithiothreitol			25 uM
5	sodium azide			0.02 %

10 3D 1H-13C NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
6	HypC	[U-95% 13C; U-95% 15N]		1 mM
7	potassium phosphate			30 mM
8	KCl			30 mM
9	dithiothreitol			25 uM
10	sodium azide			0.02 %

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