BMRB: 15159

Mouse Itch 3rd domain phosphorilated in T30

Authors

Macias, M.J., Shaw, A.Z., Martin-Malpartida, P., Morales, B., Ruiz, L., Ramirez-Espain, X., Yraola, F., Royo, M.

Assembly

phosphoit30

Entity

1. phosphoit30 (polymer, Thiol state: not present), 37 monomers, 4350.638 Da Detail

GAMGPLPPGW EKRTDSNGRV YFVNHNTRIX QWEDPRS

Formula weight

4350.638 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 94.6 %, Completeness: 95.1 %, Completeness (bb): 95.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	95.1 % (214 of 225)	95.1 % (214 of 225)
Backbone	95.8 % (69 of 72)	95.8 % (69 of 72)
Sidechain	94.8 % (145 of 153)	94.8 % (145 of 153)
Aromatic	100.0 % (23 of 23)	100.0 % (23 of 23)
Methyl	100.0 % (11 of 11)	100.0 % (11 of 11)

1. PhosphoI3

GAMGPLPPGW EKRTDSNGRV YFVNHNTRIX QWEDPRS

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 280 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	PhosphoI3	natural abundance		1.0 mM
2	Sodium Phosphate	natural abundance		20 mM
3	NaCl	natural abundance		100 mM
4	Sodium Azide	natural abundance		0.02 % v/v

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Protein Blocks Logo

Calculated from 7 models in PDB: 2JOC, Strand ID: A Detail

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Release date

2007-10-23

Citation

NMR structural studies of the ItchWW3 domain reveal that phosphorylation at T30 inhibits the interaction with PPxY-containing ligands
Morales, B., Ramirez-Espain, X., Shaw, A.Z., Martin-Malpartida, P., Yraola, F., Sanchez-Tillo, E., Farrera, C., Celada, A., Royo, M., Macias, M.J.
Structure (2007), 15, 473-483, PubMed 17437719 , DOI 10.1016/j.str.2007.03.005 , Abstract

Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

Related entities 1. phosphoit30, : 1 : 2 : 552 entities Detail

Interaction partners 1. phosphoit30, : 45 interactors Detail

Experiments performed 4 experiments Detail