BMRB: 15195

Solution Structure of an M-1 Conotoxin with a novel disulfide linkage

Authors

Fang, W., Du, W., Han, Y., Huang, F.

Assembly

M conotoxin Mr3.4

Entity

1. M conotoxin Mr3.4 (polymer, Thiol state: all disulfide bound), 16 monomers, 1752.066 Da Detail

VCCPFGGCHE LCYCCD

Formula weight

1752.066 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS2:SG	1:CYS14:SG
2	disulfide	sing	1:CYS3:SG	1:CYS12:SG
3	disulfide	sing	1:CYS8:SG	1:CYS15:SG

Source organism

Conus marmoreus

Exptl. method

solution NMR

Refine. method

distance geometry, simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 90.2 %, Completeness (bb): 100.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	90.2 % (74 of 82)	90.2 % (74 of 82)
Backbone	100.0 % (33 of 33)	100.0 % (33 of 33)
Sidechain	83.7 % (41 of 49)	83.7 % (41 of 49)
Aromatic	63.6 % (7 of 11)	63.6 % (7 of 11)
Methyl	100.0 % (4 of 4)	100.0 % (4 of 4)

1. M conotoxin Mr3.4

VCCPFGGCHE LCYCCD

Show sample condition

Sample #1

Solvent system 90% H2O, 10% D2O, Pressure 1 atm, Temperature 294 K, pH 3.0, Details 2.0mM; 0.01% TFA; 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Mr3e	natural abundance		2.0 mM
2	TFA	natural abundance		0.01 %

Sample #2

Solvent system 99.99% D2O, Pressure 1 atm, Temperature 294 K, pH 3.0, Details 2.0mM; 0.01% TFA; 99.99% D2O

#	Name	Isotope labeling	Type	Concentration
3	Mr3e	natural abundance		2.0 mM
4	TFA	natural abundance		0.01 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2EFZ, Strand ID: A Detail

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Release date

2007-06-05

Citation

Solution structure of an M-1 conotoxin with a novel disulfide linkage
Du, W., Han, Y., Huang, F., Li, J., Chi, C.W., Fang, W.
FEBS J. (2007), 274, 2596-2602, PubMed 17437523 , DOI 10.1111/j.1742-4658.2007.05795.x , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. M conotoxin Mr3.4, : 1 : 1 : 1 : 2 entities Detail

Experiments performed 6 experiments Detail