BMRB: 15197

1H, 15N, and 13C chemical shift assignments for CaBP1

Authors

Ames, J.

Assembly

CaBP1

Entity

1. CaBP1 (polymer, Thiol state: all free), 167 monomers, 19429.80 Da Detail

MGNCVKYPLR NLSRKDRSLR PEEIEELREA FREFDKDKDG YINCRDLGNC MRTMGYMPTE MELIELSQQI NMNLGGHVDF DDFVELMGPK LLAETADMIG VKELRDAFRE FDTNGDGEIS TSELREAMRK LLGHQVGHRD IEEIIRDVDL NGDGRVDFEE FVRMMSR

Formula weight

19429.8 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.0 %, Completeness: 77.2 %, Completeness (bb): 91.6 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	77.2 % (1507 of 1953)	72.4 % (746 of 1030)	81.3 % (609 of 749)	87.4 % (152 of 174)
Backbone	91.6 % (911 of 994)	92.4 % (318 of 344)	91.2 % (444 of 487)	91.4 % (149 of 163)
Sidechain	66.5 % (739 of 1112)	62.4 % (428 of 686)	74.2 % (308 of 415)	27.3 % (3 of 11)
Aromatic	31.0 % (36 of 116)	46.6 % (27 of 58)	15.5 % (9 of 58)
Methyl	92.1 % (140 of 152)	92.1 % (70 of 76)	92.1 % (70 of 76)

1. CaBP1

MGNCVKYPLR NLSRKDRSLR PEEIEELREA FREFDKDKDG YINCRDLGNC MRTMGYMPTE MELIELSQQI NMNLGGHVDF DDFVELMGPK LLAETADMIG VKELRDAFRE FDTNGDGEIS TSELREAMRK LLGHQVGHRD IEEIIRDVDL NGDGRVDFEE FVRMMSR

Show sample condition

Sample

Pressure 1 atm, Temperature 305 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	CaBP1	[U-98% 13C; U-98% 15N]		0.5 mM

Hide sample codition

LACS Plot; CA

Referencing offset: -0.29 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.29 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: -0.08 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: -0.34 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 15 models in PDB: 2K7B, Strand ID: A Detail

---

Release date

2007-04-10

Citation

1H, 15N, and 13C chemical shift assignments of calcium-binding protein 1 (CaBP1)
Li, C., Ames, J.B.
Biomol. NMR Assign. (2007), 1, 77-79, PubMed 19636832 , DOI 10.1007/s12104-007-9019-y , Abstract

Related entities 1. CaBP1, : 2 : 2 : 296 entities Detail

Interaction partners 1. CaBP1, : 4 interactors Detail

Experiments performed 6 experiments Detail