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BMRB: 15208

2JOX

Embryonic Neural Inducing Factor Churchill is not a DNA-Binding Zinc Finger Protein: Solution Structure Reveals a Solvent-Exposed Beta-Sheet and Zinc Binuclear Cluster

Authors

Lee, B.M., Buck-Koehntop, B.A., Martinez-Yamout, M.A., Gottesfeld, J.M., Dyson, H., Wright, P.E.

Assembly

Churchill

Entity

1. Churchill (polymer, Thiol state: free and disulfide bound), 106 monomers, 12149.65 Da Detail

CGDCVEKEYP NRGNTCLENG SFLLNFTGCA VCSKRDFMLI TNKSLKEEDG EEIVTYDHLC KNCHHVIARH EYTFSIMDEF QEYTMLCLLC GKAEDTISIL PDDPRQ

2. ZN (non-polymer), 65.409 × 3 Da

Total weight

12345.877 Da

Max. entity weight

12149.65 Da

Entity Connection

na 12 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	2:ZN1:ZN	1:CYS1:SG
2	na	sing	2:ZN1:ZN	1:CYS4:SG
3	na	sing	2:ZN1:ZN	1:CYS29:SG
4	na	sing	2:ZN1:ZN	1:HIS65:NE2
5	na	sing	2:ZN1:ZN	1:CYS29:SG
6	na	sing	2:ZN1:ZN	1:CYS32:SG
7	na	sing	2:ZN1:ZN	1:CYS60:SG
8	na	sing	2:ZN1:ZN	1:CYS63:SG
9	na	sing	2:ZN1:ZN	1:HIS58:NE2
10	na	sing	2:ZN1:ZN	1:HIS70:ND1
11	na	sing	2:ZN1:ZN	1:CYS87:SG
12	na	sing	2:ZN1:ZN	1:CYS90:SG

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.4 %, Completeness (bb): 98.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	97.4 % (1186 of 1218)	98.7 % (624 of 632)	95.4 % (453 of 475)	98.2 % (109 of 111)
Backbone	98.4 % (620 of 630)	98.1 % (211 of 215)	98.7 % (308 of 312)	98.1 % (101 of 103)
Sidechain	96.7 % (665 of 688)	99.0 % (413 of 417)	92.8 % (244 of 263)	100.0 % (8 of 8)
Aromatic	79.6 % (78 of 98)	95.9 % (47 of 49)	63.3 % (31 of 49)
Methyl	100.0 % (100 of 100)	100.0 % (50 of 50)	100.0 % (50 of 50)

1. Churchill

CGDCVEKEYP NRGNTCLENG SFLLNFTGCA VCSKRDFMLI TNKSLKEEDG EEIVTYDHLC KNCHHVIARH EYTFSIMDEF QEYTMLCLLC GKAEDTISIL PDDPRQ

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	Churchill	[U-13C; U-15N]	1 mM
2	Tris	natural abundance	25 mM
3	NaCl	natural abundance	100 mM
4	DTT	natural abundance	2 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.86 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2JOX, Strand ID: A Detail

Release date

2008-05-05

Citation

Embryonic neural inducing factor churchill is not a DNA-binding zinc finger protein: solution structure reveals a solvent-exposed beta-sheet and zinc binuclear cluster
Lee, B.M., Buck-Koehntop, B.A., Martinez-Yamout, M.A., Dyson, H., Wright, P.E.
J. Mol. Biol. (2007), 371, 1274-1289, PubMed 17610897 , DOI 10.1016/j.jmb.2007.06.021 , Abstract

Related entities 1. Churchill, : 1 : 2 : 9 entities Detail

Interaction partners 1. Churchill, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 17 experiments Detail

1 2D 1H-15N HSQC