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BMRB: 15210

2JOY

NMR Structure of 50S Ribosomal Protein L14e from Sulfolobus Solfataricus; Northeast Structural Genomics Consortium Target SSR105.

Authors

Singarapu, K., Wu, Y., Yee, A., semesi, A., Arrowsmith, C., Szyperski, T.

Assembly

50s ribosomal protein l14e

Entity

1. 50s ribosomal protein l14e (polymer, Thiol state: all free), 96 monomers, 10860.71 Da Detail

MPAIEVGRIC VKVKGREAGS KCVIVDIIDD NFVLVTGPKD ITGVKRRRVN ILHLEPTDKK IDIQKGASDE EVKKKLEESN LTEYMKEKIK IRMPTL

Formula weight

10860.71 Da

Source organism

Saccharolobus solfataricus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.0 %, Completeness: 97.7 %, Completeness (bb): 98.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	97.7 % (1126 of 1153)	97.9 % (597 of 610)	97.1 % (434 of 447)	99.0 % (95 of 96)
Backbone	98.4 % (559 of 568)	99.0 % (192 of 194)	97.9 % (276 of 282)	98.9 % (91 of 92)
Sidechain	97.2 % (656 of 675)	97.4 % (405 of 416)	96.9 % (247 of 255)	100.0 % (4 of 4)
Aromatic	77.3 % (17 of 22)	100.0 % (11 of 11)	54.5 % (6 of 11)
Methyl	100.0 % (124 of 124)	100.0 % (62 of 62)	100.0 % (62 of 62)

1. 50s ribosomal protein l14e

MPAIEVGRIC VKVKGREAGS KCVIVDIIDD NFVLVTGPKD ITGVKRRRVN ILHLEPTDKK IDIQKGASDE EVKKKLEESN LTEYMKEKIK IRMPTL

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	0.5 mM
2	H2O		90 %
3	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	entity	[U-10% 13C; U-99% 15N]		0.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.29 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.29 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.28 ppm, Outliers: 4 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2JOY, Strand ID: A Detail

Release date

2007-06-04

Citation

NMR Structure of 50S Ribosomal Protein L14e from Sulfolobus Solfataricus; Northeast Structural Genomics Consortium Target SSR105
Singarapu, K., Wu, Y., Yee, A., semesi, A., Arrowsmith, C., Szyperski, T.

Related entities 1. 50s ribosomal protein l14e, : 1 : 1 : 1 : 191 entities Detail

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC