BMRB: 15229

ATOMIC STRUCTURE OF TRANSLATION INITIATION FACTOR aIF2 beta-SUBUNIT FROM ARCHAEBACTERIA SULFOLOBUS SOLFATARICUS: HIGH RESOLUTION NMR IN SOLUTION

Authors

Vasile, F., Pechkova, E., Stolboushkina, E., Garber, M., Nicolini, C.

Assembly

Translation initiation factor

Entity

1. Translation initiation factor (polymer, Thiol state: all free), 146 monomers, 16799.42 Da Detail

HHHHHHMGSS EKEYVEMLDR LYSKLPEKGR KEGTQSLPNM IILNIGNTTI IRNFAEYCDR IRREDKICMK YLLKELAAPG NVDDKGELVI QGKFSSQVIN TLMERFLKAY VECSTCKSLD TILKKEKKSW YIVCLACGAQ TPVKPL

Formula weight

16799.42 Da

Source organism

Saccharolobus solfataricus

Exptl. method

solution NMR

Refine. method

the structures were obtained by torsion angle dynamic calculation and energy minimization.

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 87.7 %, Completeness: 77.3 %, Completeness (bb): 87.1 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H
All	77.3 % (715 of 925)	77.3 % (715 of 925)
Backbone	87.1 % (257 of 295)	87.1 % (257 of 295)
Sidechain	72.7 % (458 of 630)	72.7 % (458 of 630)
Aromatic	59.6 % (34 of 57)	59.6 % (34 of 57)
Methyl	82.3 % (65 of 79)	82.3 % (65 of 79)

1. Translation initiation factor 2 beta subunit

HHHHHHMGSS EKEYVEMLDR LYSKLPEKGR KEGTQSLPNM IILNIGNTTI IRNFAEYCDR IRREDKICMK YLLKELAAPG NVDDKGELVI QGKFSSQVIN TLMERFLKAY VECSTCKSLD TILKKEKKSW YIVCLACGAQ TPVKPL

Show sample condition

Sample

Solvent system 10% D2O in H2O, Pressure 1 atm, Temperature 298 K, pH 8, Details concentration 10 mg/ml, 20 mM NaCl, 10% D2O in H2O

#	Name	Isotope labeling	Type	Concentration
1	Translation initiation factor 2 beta subunit	natural abundance		0.6 mM
2	sodium phosphate	natural abundance		50 mM
3	NaCl	natural abundance		10 mM
4	D2O			10 %
5	H2O			90 %

Hide sample codition

Protein Blocks Logo

Calculated from 10 models in PDB: 2NXU, Strand ID: A Detail

---

Release date

2008-02-19

Citation

Solution structure of the beta-subunit of the translation initiation factor aIF2 from archaebacteria Sulfolobus solfataricus
Vasile, F., Pechkova, E., Nicolini, C.
Proteins (2008), 70, 1112-1115, PubMed 18004773 , DOI 10.1002/prot.21797 , Abstract

Related entities 1. Translation initiation factor, : 1 : 8 : 102 entities Detail

Interaction partners 1. Translation initiation factor, : 1 interactors Detail

Experiments performed 2 experiments Detail