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Solution structure of the ERCC1 central domain
Authors
Tripsianes, K., Folkers, G., Zheng, C., Das, D., Grinstead, J.S., Kaptein, R., Boelens, R.
Assembly
ERCC1 central
Entity
1. ERCC1 central (polymer, Thiol state: all free), 135 monomers, 15437.63 Da Detail

MAKSNSIIVS PRQRGNPVLK FVRNVPWEFG DVIPDYVLGQ STCALFLSLR YHNLHPDYIH GRLQSLGKNF ALRVLLVQVD VKDPQQALKE LAKMCILADC TLILAWSPEE AGRYLETYKA YEQKPGGLEH HHHHH


Formula weight
15437.63 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 94.1 %, Completeness: 84.0 %, Completeness (bb): 78.7 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All84.0 % (1362 of 1621)90.4 % (763 of 844)73.7 % (469 of 636)92.2 % (130 of 141)
Backbone78.7 % (625 of 794)90.7 % (245 of 270)66.2 % (263 of 397)92.1 % (117 of 127)
Sidechain89.7 % (856 of 954)90.2 % (518 of 574)88.8 % (325 of 366)92.9 % (13 of 14)
Aromatic76.4 % (113 of 148)82.4 % (61 of 74)69.4 % (50 of 72)100.0 % (2 of 2)
Methyl95.0 % (152 of 160)95.0 % (76 of 80)95.0 % (76 of 80)

1. ERCC1 central

MAKSNSIIVS PRQRGNPVLK FVRNVPWEFG DVIPDYVLGQ STCALFLSLR YHNLHPDYIH GRLQSLGKNF ALRVLLVQVD VKDPQQALKE LAKMCILADC TLILAWSPEE AGRYLETYKA YEQKPGGLEH HHHHH

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 290 K, pH 5.5


#NameIsotope labelingTypeConcentration
1ERCC1 central[U-13C; U-15N]0.8 mM
2sodium phosphatenatural abundance50 mM
3sodium chloridenatural abundance100 mM
Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 290 K, pH 5.5


#NameIsotope labelingTypeConcentration
4ERCC1 central[U-15N]0.8 mM
5sodium phosphatenatural abundance50 mM
6sodium chloridenatural abundance100 mM

LACS Plot; CA
Referencing offset: -0.08 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: -0.08 ppm, Outliers: 2 Detail
LACS Plot; HA
Referencing offset: 0.0 ppm, Outliers: 2 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2JPD, Strand ID: A Detail


Release date
2007-08-27
Citation
Analysis of the XPA and ssDNA-binding surfaces on the central domain of human ERCC1 reveals evidence for subfunctionalization
Tripsianes, K., Folkers, G.E., Zheng, C., Das, D., Grinstead, J.S., Kaptein, R., Boelens, R.
Nucleic Acids Res. (2007), 35, 5789-5798, PubMed 17720715 , DOI 10.1093/nar/gkm503 ,
Related entities 1. ERCC1 central, : 1 : 4 : 22 entities Detail
Interaction partners 1. ERCC1 central, : 22 interactors Detail
Experiments performed 5 experiments Detail
NMR combined restraints 5 contents Detail
Keywords PROTEIN