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BMRB: 15242

2JPE

FHA domain of NIPP1

Authors

Kumeta, H., Ogura, K., Fujioka, Y., Tanuma, N., Kikuchi, K., Inagaki, F.

Assembly

a single polypeptide chain

Entity

1. a single polypeptide chain (polymer, Thiol state: all free), 140 monomers, 15498.60 Da Detail

GPLGSPNSMA AAVNSGSSLP LFDCPTWAGK PPPGLHLDVV KGDKLIEKLI IDEKKYYLFG RNPDLCDFTI DHQSCSRVHA ALVYHKHLKR VFLIDLNSTH GTFLGHIRLE PHKPQQIPID STVSFGASTR AYTLREKPQT

Formula weight

15498.6 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing, torsion angle dynamics

Data set

assigned_chemical_shifts, heteronucl_NOEs

Chem. Shift Complete

Sequence coverage: 95.7 %, Completeness: 94.2 %, Completeness (bb): 93.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.2 % (1540 of 1634)	94.7 % (805 of 850)	93.8 % (607 of 647)	93.4 % (128 of 137)
Backbone	93.0 % (759 of 816)	93.2 % (259 of 278)	92.7 % (380 of 410)	93.8 % (120 of 128)
Sidechain	95.7 % (907 of 948)	95.5 % (546 of 572)	96.2 % (353 of 367)	88.9 % (8 of 9)
Aromatic	96.3 % (131 of 136)	100.0 % (68 of 68)	92.5 % (62 of 67)	100.0 % (1 of 1)
Methyl	97.4 % (152 of 156)	97.4 % (76 of 78)	97.4 % (76 of 78)

1. FHA domain

GPLGSPNSMA AAVNSGSSLP LFDCPTWAGK PPPGLHLDVV KGDKLIEKLI IDEKKYYLFG RNPDLCDFTI DHQSCSRVHA ALVYHKHLKR VFLIDLNSTH GTFLGHIRLE PHKPQQIPID STVSFGASTR AYTLREKPQT

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	FHA domain	[U-100% 13C; U-100% 15N]	0.5 mM
2	H2O		90 %
3	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.31 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.31 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 4 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.75 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2JPE, Strand ID: A Detail

Release date

2007-06-12

Citation

The NMR structure of the NIPP1 FHA domain
Kumeta, H., Ogura, K., Adachi, S., Fujioka, Y., Tanuma, K., Kikuchi, K., Inagaki, F.
J. Biomol. NMR (2008), 40, 219-224, PubMed 18253837 , DOI 10.1007/s10858-008-9222-x , Abstract

Related entities 1. a single polypeptide chain, : 1 : 6 : 39 entities Detail

Interaction partners 1. a single polypeptide chain, : 7 interactors Detail

More details for 7 interactors identified by 2 citations

Experiments performed 16 experiments Detail

1 2D 1H-15N HSQC