BMRB: 15245

NMR SOLUTION STRUCTURE OF THE VILLIN HEADPIECE MUTANT G34L

Authors

Gronwald, W., Hohm, T., Hoffmann, D.

Assembly

villin G34L monomer

Entity

1. villin G34L monomer (polymer, Thiol state: not present), 36 monomers, 4245.958 Da Detail

MLSDEDFKAV FGMTRSAFAN LPLWKQQNLK KEKLLF

Formula weight

4245.958 Da

Source organism

Gallus gallus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.2 %, Completeness: 76.3 %, Completeness (bb): 97.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	76.3 % (187 of 245)	76.3 % (187 of 245)
Backbone	97.2 % (70 of 72)	97.2 % (70 of 72)
Sidechain	67.6 % (117 of 173)	67.6 % (117 of 173)
Aromatic	57.7 % (15 of 26)	57.7 % (15 of 26)
Methyl	88.9 % (16 of 18)	88.9 % (16 of 18)

1. villin G34L

MLSDEDFKAV FGMTRSAFAN LPLWKQQNLK KEKLLF

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.0, Details pH 5.0

#	Name	Isotope labeling	Type	Concentration
1	villin_G34L	natural abundance		4.3 mg
2	H2O	natural abundance		90 % v/v
3	D2O	natural abundance		10 % v/v
4	DSS	natural abundance		0.1 mM

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Protein Blocks Logo

Calculated from 10 models in PDB: 2PPZ, Strand ID: A Detail

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Release date

2007-05-13

Citation

Evolutionary Pareto-optimization of stably folding peptides
Gronwald, W., Hohm, T., Hoffmann, D.
BMC Bioinformatics (2008), 9, 109-109, PubMed 18284690 , DOI 10.1186/1471-2105-9-109 , Abstract

Related entities 1. villin G34L monomer, : 1 : 12 : 131 entities Detail

Interaction partners 1. villin G34L monomer, : 1 interactors Detail

Experiments performed 2 experiments Detail