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BMRB: 15247

2V9H

1H, 13C and 15N resonance assignments of the Escherichia coli YaeT POTRA domain

Authors

Knowles, T.J., Bobat, S., Jeeves, M., Henderson, I.R., Overduin, M.

Assembly

POTRA

Entity

1. POTRA (polymer, Thiol state: not present), 164 monomers, 17967.05 Da Detail

AEGFVVKDIH FEGLQRVAVG AALLSMPVRT GDTVNDEDIS NTIRALFATG NFEDVRVLRD GDTLLVQVKE RPTIASITFS GNKSVKDDML KQNLEASGVR VGESLDRTTI ADIEKGLEDF YYSVGKYSAS VKAVVTPLPR NRVDLKLVFQ EGVSLQHHHH QTAG

Formula weight

17967.05 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Refine. method

DISTANCE GEOMETRY

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.7 %, Completeness: 93.4 %, Completeness (bb): 94.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.4 % (1729 of 1852)	92.9 % (884 of 952)	93.5 % (681 of 728)	95.3 % (164 of 172)
Backbone	94.6 % (923 of 976)	94.7 % (319 of 337)	94.4 % (452 of 479)	95.0 % (152 of 160)
Sidechain	92.5 % (950 of 1027)	91.9 % (565 of 615)	93.3 % (373 of 400)	100.0 % (12 of 12)
Aromatic	73.7 % (84 of 114)	73.7 % (42 of 57)	73.7 % (42 of 57)
Methyl	95.3 % (204 of 214)	96.3 % (103 of 107)	94.4 % (101 of 107)

1. POTRA

AEGFVVKDIH FEGLQRVAVG AALLSMPVRT GDTVNDEDIS NTIRALFATG NFEDVRVLRD GDTLLVQVKE RPTIASITFS GNKSVKDDML KQNLEASGVR VGESLDRTTI ADIEKGLEDF YYSVGKYSAS VKAVVTPLPR NRVDLKLVFQ EGVSLQHHHH QTAG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	POTRA	[U-99% 13C; U-99% 15N]	0.8 mM
2	MES	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	sodium azide	natural abundance	3 mM
5	Complete protease inhibitor cocktail	natural abundance

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.28 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.28 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2V9H, Strand ID: A Detail

Release date

2007-08-21

Citation

Secondary structure and 1H, 13C and 15N resonance assignments of the Escherichia coli YaeT POTRA domain
Knowles, T.J., Bobat, S., Jeeves, M., Henderson, I.R., Overduin, M.
Biomol. NMR Assign. (2007), 1, 113-115, PubMed 19636842 , DOI 10.1007/s12104-007-9032-1 , Abstract

Related entities 1. POTRA, : 1 : 2 : 93 entities Detail

Interaction partners 1. POTRA, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 9 experiments Detail

1 3D HNCA