BMRBj - BMREntryMaster

Found 1 Document (0.711 seconds)

BMRB: 15257

2JPP

Structural basis of RsmA/CsrA RNA recognition: Structure of RsmE bound to the Shine-Dalgarno sequence of hcnA mRNA

Authors

Schubert, M., Lapouge, K., Duss, O., Oberstrass, F.C., Jelesarov, I., Haas, D., Allain, F.H.-T.

Assembly

RsmE dimer with two RNA stem loops

Entity

1. hncA mRNA SD 20mer (polymer, Thiol state: not present), 20 monomers, 6500.866 × 2 Da Detail

GGGCUUCACG GAUGAAGCCC

2. RsmE (polymer, Thiol state: not present), 70 monomers, 7832.828 × 2 Da Detail

MLILTRKVGE SINIGDDITI TILGVSGQQV RIGINAPKDV AVHREEIYQR IQAGLTAPDK RETPHHHHHH

Total weight

28667.389 Da

Max. entity weight

7832.828 Da

Source organism

Pseudomonas fluorescens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.4 %, Completeness: 83.4 %, Completeness (bb): 83.1 % Detail

Polymer type: polyribonucleotide polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	83.4 % (937 of 1123)	87.5 % (519 of 593)	77.0 % (344 of 447)	89.2 % (74 of 83)
Backbone	83.1 % (527 of 634)	92.0 % (242 of 263)	74.0 % (225 of 304)	89.6 % (60 of 67)
Sidechain	84.4 % (467 of 553)	83.9 % (277 of 330)	85.0 % (176 of 207)	87.5 % (14 of 16)
Aromatic	71.3 % (87 of 122)	77.0 % (47 of 61)	62.7 % (32 of 51)	80.0 % (8 of 10)
Methyl	94.7 % (89 of 94)	93.6 % (44 of 47)	95.7 % (45 of 47)

1. hncA mRNA SD 20mer

GGGCUUCACG GAUGAAGCCC

2. RsmE

MLILTRKVGE SINIGDDITI TILGVSGQQV RIGINAPKDV AVHREEIYQR IQAGLTAPDK RETPHHHHHH

Show sample condition

Sample #1

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 313.15 K, pH 7.2, Details 15N RsmE - unlabeled RNA

#	Name	Isotope labeling	Concentration
1	RsmE	[U-100% 15N]	1 mM
2	hncA_mRNA_SD_20mer	natural abundance	1 mM
3	H2O	natural abundance	97 %
4	D2O		3 %
5	NaCl		30 mM
6	K2HPO4		50 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 313.15 K, pH 7.2, Details 15N/13C RsmE - unlabeled RNA

#	Name	Isotope labeling	Concentration
7	RsmE	[U-100% 13C; U-100% 15N]	1 mM
8	hncA_mRNA_SD_20mer	natural abundance	1 mM
9	D2O		100 %
10	NaCl		30 mM
11	K2HPO4		50 mM

Sample #3

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 313.15 K, pH 7.2, Details 15N/13C RsmE - unlabeled RNA

#	Name	Isotope labeling	Concentration
12	RsmE	[U-100% 13C; U-100% 15N]	1 mM
13	hncA_mRNA_SD_20mer	natural abundance	1 mM
14	H2O	natural abundance	97 %
15	D2O		3 %
16	NaCl		30 mM
17	K2HPO4		50 mM

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 313.15 K, pH 7.2, Details 15N RsmE - unlabelled RNA

#	Name	Isotope labeling	Concentration
18	RsmE	[U-100% 15N]	1 mM
19	hncA_mRNA_SD_20mer	natural abundance	1 mM
20	D2O		100 %
21	NaCl		30 mM
22	K2HPO4		50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2JPP, Strand ID: A, B Detail

Release date

2007-09-11

Citation

Molecular basis of messenger RNA recognition by the specific bacterial repressing clamp RsmA/CsrA
Schubert, M., Lapouge, K., Duss, O., Oberstrass, F.C., Jelesarov, I., Haas, D., Allain, F.H.-T.
Nat. Struct. Mol. Biol. (2007), 14, 807-813, PubMed 17704818 , DOI 10.1038/nsmb1285 , Abstract

Related entities 1. hncA mRNA SD 20mer, : 1 entities Detail

Related entities 2. RsmE, : 1 : 7 : 27 entities Detail

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 313.15 K, pH 7.2, Details 15N RsmE - unlabeled RNA

#	Name	Isotope labeling	Concentration
1	RsmE	[U-100% 15N]	1 mM
2	hncA_mRNA_SD_20mer	natural abundance	1 mM
3	H2O	natural abundance	97 %
4	D2O		3 %
5	NaCl		30 mM
6	K2HPO4		50 mM

2 3D 1H-15N NOESY

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 313.15 K, pH 7.2, Details 15N RsmE - unlabeled RNA

#	Name	Isotope labeling	Concentration
1	RsmE	[U-100% 15N]	1 mM
2	hncA_mRNA_SD_20mer	natural abundance	1 mM
3	H2O	natural abundance	97 %
4	D2O		3 %
5	NaCl		30 mM
6	K2HPO4		50 mM

3 3D CBCA(CO)NH

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 313.15 K, pH 7.2, Details 15N/13C RsmE - unlabeled RNA

#	Name	Isotope labeling	Concentration
12	RsmE	[U-100% 13C; U-100% 15N]	1 mM
13	hncA_mRNA_SD_20mer	natural abundance	1 mM
14	H2O	natural abundance	97 %
15	D2O		3 %
16	NaCl		30 mM
17	K2HPO4		50 mM

4 3D HNCA

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 313.15 K, pH 7.2, Details 15N/13C RsmE - unlabeled RNA

#	Name	Isotope labeling	Concentration
12	RsmE	[U-100% 13C; U-100% 15N]	1 mM
13	hncA_mRNA_SD_20mer	natural abundance	1 mM
14	H2O	natural abundance	97 %
15	D2O		3 %
16	NaCl		30 mM
17	K2HPO4		50 mM

5 3D HNCACB

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 313.15 K, pH 7.2, Details 15N/13C RsmE - unlabeled RNA

#	Name	Isotope labeling	Concentration
12	RsmE	[U-100% 13C; U-100% 15N]	1 mM
13	hncA_mRNA_SD_20mer	natural abundance	1 mM
14	H2O	natural abundance	97 %
15	D2O		3 %
16	NaCl		30 mM
17	K2HPO4		50 mM

6 3D HNCO

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 313.15 K, pH 7.2, Details 15N/13C RsmE - unlabeled RNA

#	Name	Isotope labeling	Concentration
12	RsmE	[U-100% 13C; U-100% 15N]	1 mM
13	hncA_mRNA_SD_20mer	natural abundance	1 mM
14	H2O	natural abundance	97 %
15	D2O		3 %
16	NaCl		30 mM
17	K2HPO4		50 mM

7 2D 1H-1H TOCSY

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 313.15 K, pH 7.2, Details 15N RsmE - unlabeled RNA

#	Name	Isotope labeling	Concentration
1	RsmE	[U-100% 15N]	1 mM
2	hncA_mRNA_SD_20mer	natural abundance	1 mM
3	H2O	natural abundance	97 %
4	D2O		3 %
5	NaCl		30 mM
6	K2HPO4		50 mM

8 3D H(CCO)NH

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 313.15 K, pH 7.2, Details 15N/13C RsmE - unlabeled RNA

#	Name	Isotope labeling	Concentration
12	RsmE	[U-100% 13C; U-100% 15N]	1 mM
13	hncA_mRNA_SD_20mer	natural abundance	1 mM
14	H2O	natural abundance	97 %
15	D2O		3 %
16	NaCl		30 mM
17	K2HPO4		50 mM

9 2D 1H-1H NOESY

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 313.15 K, pH 7.2, Details 15N RsmE - unlabeled RNA

#	Name	Isotope labeling	Concentration
1	RsmE	[U-100% 15N]	1 mM
2	hncA_mRNA_SD_20mer	natural abundance	1 mM
3	H2O	natural abundance	97 %
4	D2O		3 %
5	NaCl		30 mM
6	K2HPO4		50 mM

10 2D 1H-13C HSQC

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313.15 K, pH 7.2, Details 15N/13C RsmE - unlabeled RNA

#	Name	Isotope labeling	Concentration
7	RsmE	[U-100% 13C; U-100% 15N]	1 mM
8	hncA_mRNA_SD_20mer	natural abundance	1 mM
9	D2O		100 %
10	NaCl		30 mM
11	K2HPO4		50 mM

11 3D 1H-13C NOESY

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 313.15 K, pH 7.2, Details 15N/13C RsmE - unlabeled RNA

#	Name	Isotope labeling	Concentration
12	RsmE	[U-100% 13C; U-100% 15N]	1 mM
13	hncA_mRNA_SD_20mer	natural abundance	1 mM
14	H2O	natural abundance	97 %
15	D2O		3 %
16	NaCl		30 mM
17	K2HPO4		50 mM

12 3D HN(CO)CA

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 313.15 K, pH 7.2, Details 15N/13C RsmE - unlabeled RNA

#	Name	Isotope labeling	Concentration
12	RsmE	[U-100% 13C; U-100% 15N]	1 mM
13	hncA_mRNA_SD_20mer	natural abundance	1 mM
14	H2O	natural abundance	97 %
15	D2O		3 %
16	NaCl		30 mM
17	K2HPO4		50 mM

nullKeywords CsrA, PROTEIN/RNA, RNA recognition, RsmA, Shine-Dalgarno

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Found 1 Document (0.711 seconds)

BMRB: 15257

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. hncA mRNA SD 20mer, : 1 entities Detail

Related entities 2. RsmE, : 1 : 7 : 27 entities Detail

Experiments performed 12 experiments Detail

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