BMRB: 15259

LactococcinGa in DPC and TFE

Authors

Rogne, P., Fimland, G., Nissen-Meyer, J., Kristiansen, P.E.

Assembly

lcnGa

Entity

1. lcnGa (polymer, Thiol state: not present), 39 monomers, 4309.804 Da Detail

GTWDDIGQGI GRVAYWVGKA LGNLSDVNQA SRINRKKKH

Formula weight

4309.804 Da

Source organism

Lactococcus lactis

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 100.0 %, Completeness: 95.7 %, Completeness (bb): 98.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	95.7 % (270 of 282)	95.8 % (226 of 236)	95.7 % (44 of 46)
Backbone	98.4 % (121 of 123)	98.8 % (83 of 84)	97.4 % (38 of 39)
Sidechain	93.7 % (149 of 159)	94.1 % (143 of 152)	85.7 % (6 of 7)
Aromatic	90.0 % (18 of 20)	88.9 % (16 of 18)	100.0 % (2 of 2)
Methyl	100.0 % (20 of 20)	100.0 % (20 of 20)

1. lcnGa

GTWDDIGQGI GRVAYWVGKA LGNLSDVNQA SRINRKKKH

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 2.8, Details LcnGa:DPC 1:200 peptide to lipid ratio

#	Name	Isotope labeling	Type	Concentration
1	lcnGa	[U-15N]		1 mM
2	DPC	[U-2H]		200 mM
3	TFA	natural abundance		0.1 %
4	D2O	natural abundance		10 %
5	DSS	natural abundance		0.2 mM

Sample #2

Solvent system 90% TFE/10% H2O, Pressure 1 atm, Temperature 298 K, pH 2.8, Details LactococcinG alpha in TFE

#	Name	Isotope labeling	Type	Concentration
6	lcnG a TFE	[U-15N]		1 mM
7	TFE	[U-2H]		90 %
8	TFA	natural abundance		0.1 %
9	DSS	natural abundance		0.2 mM

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Chem. Shift Complete2

Sequence coverage: 97.4 %, Completeness: 92.0 %, Completeness (bb): 95.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	92.0 % (519 of 564)	91.3 % (431 of 472)	95.7 % (88 of 92)
Backbone	95.9 % (236 of 246)	95.2 % (160 of 168)	97.4 % (76 of 78)
Sidechain	89.0 % (283 of 318)	89.1 % (271 of 304)	85.7 % (12 of 14)
Aromatic	95.0 % (38 of 40)	94.4 % (34 of 36)	100.0 % (4 of 4)
Methyl	90.0 % (36 of 40)	90.0 % (36 of 40)

1. lcnGa

GTWDDIGQGI GRVAYWVGKA LGNLSDVNQA SRINRKKKH

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 2.8, Details LcnGa:DPC 1:200 peptide to lipid ratio

#	Name	Isotope labeling	Type	Concentration
1	lcnGa	[U-15N]		1 mM
2	DPC	[U-2H]		200 mM
3	TFA	natural abundance		0.1 %
4	D2O	natural abundance		10 %
5	DSS	natural abundance		0.2 mM

Sample #2

Solvent system 90% TFE/10% H2O, Pressure 1 atm, Temperature 298 K, pH 2.8, Details LactococcinG alpha in TFE

#	Name	Isotope labeling	Type	Concentration
6	lcnG a TFE	[U-15N]		1 mM
7	TFE	[U-2H]		90 %
8	TFA	natural abundance		0.1 %
9	DSS	natural abundance		0.2 mM

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Protein Blocks Logo

Calculated from 20 models in PDB: 2JPJ, Strand ID: A Detail

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Release date

2008-02-20

Citation

Three-dimensional structure of the two peptides that constitute the two-peptide bacteriocin lactococcin G
Rogne, P., Fimland, G., Nissen-Meyer, J., Kristiansen, P.E.
Biochim. Biophys. Acta (2008), 1784, 543-554, PubMed 18187052 , DOI 10.1016/j.bbapap.2007.12.002 , Abstract

Related entities 1. lcnGa, : 1 : 1 : 1 : 1 entities Detail

Experiments performed 10 experiments Detail