BMRBj - BMREntryMaster

Found 1 Document (0.762 seconds)

BMRB: 15270

2JQ5

Solution structure of RPA3114, a SEC-C motif containing protein from Rhodopseudomonas palustris; Northeast Structural Genomics Consortium target RpT5 / Ontario Center for Structural Proteomics target RP3097

Authors

Lukin, J., Gutmanas, A., Karra, M., Semesi, A.

Assembly

RP3097

Entity

1. RP3097 (polymer, Thiol state: all other bound), 128 monomers, 14638.08 Da Detail

MNCVCGSGKT YDDCCGPLLA RTRSAASPEA LMRSRYAAYA LKDFDYIVET TDPERRDLFD HDVNRAWMEE SDFLELRVLG SSEKGSRGTV EFIARFRRGG GPEQSHHERS QFRKARGRWY FSEGEAVD

2. ZN (non-polymer), 65.409 Da

Total weight

14703.489 Da

Max. entity weight

14638.08 Da

Source organism

Rhodopseudomonas palustris

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 92.2 %, Completeness (bb): 95.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.2 % (1340 of 1453)	92.0 % (699 of 760)	91.8 % (517 of 563)	95.4 % (124 of 130)
Backbone	95.8 % (728 of 760)	95.8 % (252 of 263)	95.7 % (357 of 373)	96.0 % (119 of 124)
Sidechain	89.8 % (727 of 810)	89.9 % (447 of 497)	89.6 % (275 of 307)	83.3 % (5 of 6)
Aromatic	71.9 % (105 of 146)	76.7 % (56 of 73)	67.6 % (48 of 71)	50.0 % (1 of 2)
Methyl	99.0 % (95 of 96)	97.9 % (47 of 48)	100.0 % (48 of 48)

1. RP3097

MNCVCGSGKT YDDCCGPLLA RTRSAASPEA LMRSRYAAYA LKDFDYIVET TDPERRDLFD HDVNRAWMEE SDFLELRVLG SSEKGSRGTV EFIARFRRGG GPEQSHHERS QFRKARGRWY FSEGEAVD

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	entity_1	[U-13C; U-15N]	0.5 mM
2	TRIS	[U-99% 2H]	10 mM
3	DTT	[U-99% 2H]	10 mM
4	sodium chloride	natural abundance	300 mM
5	sodium azide	natural abundance	0.1 %
6	benzamidine	natural abundance	1 mM
7	ZnS04	natural abundance	10 uM
8	H2O		95 %
9	D2O		5 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
10	entity_1	[U-13C; U-15N]	0.5 mM
11	TRIS	[U-99% 2H]	10 mM
12	DTT	[U-99% 2H]	10 mM
13	sodium chloride	natural abundance	300 mM
14	sodium azide	natural abundance	0.1 %
15	benzamidine	natural abundance	1 mM
16	ZnS04	natural abundance	10 uM
17	D2O		100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.19 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.19 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.29 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2JQ5, Strand ID: A Detail

Release date

2007-07-16

Citation

Solution structure of a Se-C motif containing protein from Rhodopseudomonas palustris
Lemak, A., Lukin, J., Yee, A., Gutmanas, A., Karra, M., Semesi, A., Arrowsmith, C.H.
Not known

Related entities 1. RP3097, : 1 : 72 entities Detail

Experiments performed 10 experiments Detail

1 3D HNCA