BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	73.8 % (1073 of 1454)	71.3 % (554 of 777)	75.8 % (423 of 558)	80.7 % (96 of 119)
Backbone	81.8 % (556 of 680)	83.5 % (193 of 231)	80.5 % (273 of 339)	81.8 % (90 of 110)
Sidechain	69.0 % (609 of 883)	66.1 % (361 of 546)	73.8 % (242 of 328)	66.7 % (6 of 9)
Aromatic	43.8 % (28 of 64)	65.6 % (21 of 32)	21.9 % (7 of 32)
Methyl	90.5 % (105 of 116)	91.4 % (53 of 58)	89.7 % (52 of 58)

1. af54

MEKGTFTLKD IYKQIEAMNK MGPVRKIFEM LPFGLGLKVD NDVMEMTQEK MKKFRVIMDS MTEEELLNPK IIDSSRIRRI AIGSGTSPQE VKELLNYYKT MKNLMKKMKK NKLPI

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 300 (±0.2) K, pH 5.5 (±0.05), Details 15N_af54 sample in 25 mM sodium phosphate, 0.02% (w/v) sodium azide, pH 5.5, 0.5 mM

#	Name	Isotope labeling	Concentration
1	af54	[U-95% 15N]	0.5 (±0.05) mM
2	sodium phosphate	natural abundance	25 mM
3	sodium azide	natural abundance	0.02 % w/v
4	H2O	natural abundance	95 %
5	D2O		5 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 300 (±0.2) K, pH 5.5 (±0.05), Details 13C,15N_af54 sample in 25 mM sodium phosphate, 0.02% (w/v) sodium azide, pH 5.5, 0.5 mM

#	Name	Isotope labeling	Concentration
6	af54	[U-95% 13C; U-95% 15N]	0.5 (±0.05) mM
7	af54	[U-95% 15N]	0.5 (±0.05) mM
8	sodium phosphate	natural abundance	25 mM
9	sodium azide	natural abundance	0.02 % w/v
10	H2O	natural abundance	95 %
11	D2O		5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.23 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.23 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.27 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2JQE, Strand ID: A Detail

Release date

2008-07-14

Citation

A. fulgidus SRP54 M-domain
Ilangovan, U., Bhuiyan, S.H., Hinck, C.S., Hoyle, J.T., Pakhomova, O.N., Zwieb, C., Hinck, A.P.
J. Biomol. NMR (2008), 41, 241-248, PubMed 18618268 , DOI 10.1007/s10858-008-9252-4 , Abstract

Related entities 1. srp54 of archaeoglobus fulgius, : 1 : 1 : 171 entities Detail

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 300 (±0.2) K, pH 5.5 (±0.05), Details 15N_af54 sample in 25 mM sodium phosphate, 0.02% (w/v) sodium azide, pH 5.5, 0.5 mM

#	Name	Isotope labeling	Concentration
1	af54	[U-95% 15N]	0.5 (±0.05) mM
2	sodium phosphate	natural abundance	25 mM
3	sodium azide	natural abundance	0.02 % w/v
4	H2O	natural abundance	95 %
5	D2O		5 %

2 3D HNHA

Instrument

Bruker DRX - 600 MHz equipped with cryo probe

Sample

#	Name	Isotope labeling	Concentration
1	af54	[U-95% 15N]	0.5 (±0.05) mM
2	sodium phosphate	natural abundance	25 mM
3	sodium azide	natural abundance	0.02 % w/v
4	H2O	natural abundance	95 %
5	D2O		5 %

3 3D HNHB

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	af54	[U-95% 15N]	0.5 (±0.05) mM
2	sodium phosphate	natural abundance	25 mM
3	sodium azide	natural abundance	0.02 % w/v
4	H2O	natural abundance	95 %
5	D2O		5 %

4 3D 1H-15N NOESY

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	af54	[U-95% 15N]	0.5 (±0.05) mM
2	sodium phosphate	natural abundance	25 mM
3	sodium azide	natural abundance	0.02 % w/v
4	H2O	natural abundance	95 %
5	D2O		5 %

5 2D 1H-13C HSQC

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 300 (±0.2) K, pH 5.5 (±0.05), Details 13C,15N_af54 sample in 25 mM sodium phosphate, 0.02% (w/v) sodium azide, pH 5.5, 0.5 mM

#	Name	Isotope labeling	Concentration
6	af54	[U-95% 13C; U-95% 15N]	0.5 (±0.05) mM
7	af54	[U-95% 15N]	0.5 (±0.05) mM
8	sodium phosphate	natural abundance	25 mM
9	sodium azide	natural abundance	0.02 % w/v
10	H2O	natural abundance	95 %
11	D2O		5 %

6 3D HNCACB

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
6	af54	[U-95% 13C; U-95% 15N]	0.5 (±0.05) mM
7	af54	[U-95% 15N]	0.5 (±0.05) mM
8	sodium phosphate	natural abundance	25 mM
9	sodium azide	natural abundance	0.02 % w/v
10	H2O	natural abundance	95 %
11	D2O		5 %

7 3D CBCA(CO)NH

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
6	af54	[U-95% 13C; U-95% 15N]	0.5 (±0.05) mM
7	af54	[U-95% 15N]	0.5 (±0.05) mM
8	sodium phosphate	natural abundance	25 mM
9	sodium azide	natural abundance	0.02 % w/v
10	H2O	natural abundance	95 %
11	D2O		5 %

8 3D C(CO)NH

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
6	af54	[U-95% 13C; U-95% 15N]	0.5 (±0.05) mM
7	af54	[U-95% 15N]	0.5 (±0.05) mM
8	sodium phosphate	natural abundance	25 mM
9	sodium azide	natural abundance	0.02 % w/v
10	H2O	natural abundance	95 %
11	D2O		5 %

9 3D HCCH-TOCSY

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
6	af54	[U-95% 13C; U-95% 15N]	0.5 (±0.05) mM
7	af54	[U-95% 15N]	0.5 (±0.05) mM
8	sodium phosphate	natural abundance	25 mM
9	sodium azide	natural abundance	0.02 % w/v
10	H2O	natural abundance	95 %
11	D2O		5 %

10 3D 1H-13C NOESY

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
6	af54	[U-95% 13C; U-95% 15N]	0.5 (±0.05) mM
7	af54	[U-95% 15N]	0.5 (±0.05) mM
8	sodium phosphate	natural abundance	25 mM
9	sodium azide	natural abundance	0.02 % w/v
10	H2O	natural abundance	95 %
11	D2O		5 %

11 3D HNCO

Instrument

Bruker DRX - 600 MHz equipped with cryo probe

Sample

#	Name	Isotope labeling	Concentration
6	af54	[U-95% 13C; U-95% 15N]	0.5 (±0.05) mM
7	af54	[U-95% 15N]	0.5 (±0.05) mM
8	sodium phosphate	natural abundance	25 mM
9	sodium azide	natural abundance	0.02 % w/v
10	H2O	natural abundance	95 %
11	D2O		5 %

12 3D HN(CA)CO

Instrument

Bruker DRX - 600 MHz equipped with cryo probe

Sample

#	Name	Isotope labeling	Concentration
6	af54	[U-95% 13C; U-95% 15N]	0.5 (±0.05) mM
7	af54	[U-95% 15N]	0.5 (±0.05) mM
8	sodium phosphate	natural abundance	25 mM
9	sodium azide	natural abundance	0.02 % w/v
10	H2O	natural abundance	95 %
11	D2O		5 %

NMR combined restraints 3 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints	combined_15275_2jqe.nef
Input source #2: Coordindates	2jqe.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

---310-----320-------330-------340-------350-------360-------370-------380-------390-------400------
GSGTMEKGTFTLKDIYKQIEAMNKMGPVRKIFEMLPFGLGLKVDNDVMEMTQEKMKKFRVIMDSMTEEELLNPKIIDSSRIRRIAIGSGTSPQEVKELLN
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
GSGTMEKGTFTLKDIYKQIEAMNKMGPVRKIFEMLPFGLGLKVDNDVMEMTQEKMKKFRVIMDSMTEEELLNPKIIDSSRIRRIAIGSGTSPQEVKELLN
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100

-410-------420-----
YYKTMKNLMKKMKKNKLPI
|||||||||||||||||||
YYKTMKNLMKKMKKNKLPI
-------110---------

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	119	0	0	100.0

Content subtype: combined_15275_2jqe.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	983		79.8 (chain: A, length: 119)
1	Distance restraints	CNS/XPLOR_distance_constraints_2	Warning	1966 (1)	noe	66.4 (chain: A, length: 119)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 119, Sequence coverage: 79.8%
- Total number of assignments
  - ¹H chemical shifts: 496
  - ¹³C chemical shifts: 397
  - ¹⁵N chemical shifts: 90
- Completeness of assignments
  - Entity_assemble_ID: A
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	791	496	62.7
¹³C chemical shifts	569	397	69.8
¹⁵N chemical shifts	128	90	70.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	241	180	74.7
¹³C chemical shifts	238	174	73.1
¹⁵N chemical shifts	114	84	73.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	550	316	57.5
¹³C chemical shifts	331	223	67.4
¹⁵N chemical shifts	14	6	42.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	71	49	69.0
¹³C chemical shifts	71	47	66.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	32	16	50.0
¹³C chemical shifts	32	6	18.8

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_2
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 119, Sequence coverage: 66.4%
- Total number of restraints: 1936
- Weight of restraints: 1.0 (1936)
- Potential type of restraints: square-well-parabolic (1936)
- Range of distance target values: 2.3, 5.05 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map

Keywords af54, dynamics, NMR, protein, solution, srp54, structure, nmr structure

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (0.688 seconds)

BMRB: 15275

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. srp54 of archaeoglobus fulgius, : 1 : 1 : 171 entities Detail

Experiments performed 12 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

NMR combined restraints 3 contents Detail